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The map label for this gene is algU [H]

Identifier: 220915705

GI number: 220915705

Start: 659128

End: 659634

Strand: Reverse

Name: algU [H]

Synonym: A2cp1_0586

Alternate gene names: 220915705

Gene position: 659634-659128 (Counterclockwise)

Preceding gene: 220915712

Following gene: 220915704

Centisome position: 13.12

GC content: 75.35

Gene sequence:

>507_bases
GTGACGGCGAGCCACGACGACGACATCGGGCGGATCGTCCGGGAGACCGCCCCGCGGCTATACCGCCTCGCGGCGCGCCT
CACCGGCGACCTCGCGGAGGCCGAGGACGTGCTGCAGGAGAGCTACGTGCGAGCGTGCGCCGGGCTGGGCGGACGCGCGT
TCCAGGGTCGCTCGTCGGTCGAGACCTGGCTGCACCGCATCGTGGTGAACGCCTCGCTCGACGCGCTCCGCGCCCGCCGC
CGCCGGAGCGTCCGCGAGACGCACGCCGCGCCGGCCGACGAGGTGCTCGACCCGGGCGCCTCGCTCGAGGCCCGCGCCGC
GCTGCGCGAGCTGTCGGAGTGGCTGGACGAGCTGCCTGCGGAGCAGCGGGTGGCGCTGGTGCTGAAGGAGCTCGAGGGGC
ACAGCACCGCCGAGGTGGCGACGCTGCTGGGCTGCTCGGAGGGCGCGGTGGAGCAGCGGCTGGTGCGCGCGCGAGCGTTT
CTGCGCGAGAGGAGGAAGCATGCGTGA

Upstream 100 bases:

>100_bases
CGCCTCCGTTAGAGGATGGGCGCCCGGAACGGCCGTAGGGTCGGCCCGGGCGGCGGCGTCCAAGCCGTCGAGGTGTTCGG
GACCGGAGCGGCGGAGCGGC

Downstream 100 bases:

>100_bases
CCATGACGAGACCGACGACGCCCGCCTCGGCCGGCTCCGGGACGCCACCGGGGCGCTCCATCCCTCCGCGGCGGTCGTCG
CGAGGCTGGAGGCGGCGATC

Product: ECF subfamily RNA polymerase sigma-24 subunit

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTASHDDDIGRIVRETAPRLYRLAARLTGDLAEAEDVLQESYVRACAGLGGRAFQGRSSVETWLHRIVVNASLDALRARR
RRSVRETHAAPADEVLDPGASLEARAALRELSEWLDELPAEQRVALVLKELEGHSTAEVATLLGCSEGAVEQRLVRARAF
LRERRKHA

Sequences:

>Translated_168_residues
MTASHDDDIGRIVRETAPRLYRLAARLTGDLAEAEDVLQESYVRACAGLGGRAFQGRSSVETWLHRIVVNASLDALRARR
RRSVRETHAAPADEVLDPGASLEARAALRELSEWLDELPAEQRVALVLKELEGHSTAEVATLLGCSEGAVEQRLVRARAF
LRERRKHA
>Mature_167_residues
TASHDDDIGRIVRETAPRLYRLAARLTGDLAEAEDVLQESYVRACAGLGGRAFQGRSSVETWLHRIVVNASLDALRARRR
RSVRETHAAPADEVLDPGASLEARAALRELSEWLDELPAEQRVALVLKELEGHSTAEVATLLGCSEGAVEQRLVRARAFL
RERRKHA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=148, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 18600; Mature: 18469

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASHDDDIGRIVRETAPRLYRLAARLTGDLAEAEDVLQESYVRACAGLGGRAFQGRSSV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
ETWLHRIVVNASLDALRARRRRSVRETHAAPADEVLDPGASLEARAALRELSEWLDELPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCH
EQRVALVLKELEGHSTAEVATLLGCSEGAVEQRLVRARAFLRERRKHA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TASHDDDIGRIVRETAPRLYRLAARLTGDLAEAEDVLQESYVRACAGLGGRAFQGRSSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
ETWLHRIVVNASLDALRARRRRSVRETHAAPADEVLDPGASLEARAALRELSEWLDELPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCH
EQRVALVLKELEGHSTAEVATLLGCSEGAVEQRLVRARAFLRERRKHA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]