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The map label for this gene is exoA [H]

Identifier: 220915579

GI number: 220915579

Start: 527560

End: 528354

Strand: Reverse

Name: exoA [H]

Synonym: A2cp1_0460

Alternate gene names: 220915579

Gene position: 528354-527560 (Counterclockwise)

Preceding gene: 220915581

Following gene: 220915577

Centisome position: 10.51

GC content: 73.46

Gene sequence:

>795_bases
GTGAAGATCGGCACCTGGAACGTGAACGGCATCCGCGCGCGCGAGGCGCAGGTGGGCGAGTTCATCGCCCGCGAGGGCCC
CGACGTCGTCTGCCTGCAGGAGATCAAGGCGCCTCCGTCGAAGGTCCCGCCCTCGCTCTGCGAGCTCGAGGGCTACTGGT
GCCTGTGGCACGGCGAGACCGCCTACTCCGGCGTGGCGCTGCTGGTGCGCAAGGCGATCGCACCCGAGCGGCCGGCCTTC
GTCCACCCGCCGTTCGACCTCGAGACCCGCATCGCCACCTGCGAGCTGGGCGGCGTCACGGTGGCGTCGATCTACGTCCC
GAACGGCGGGAAGGACCTCGCCGCCAAGCTCCGCTTCCTCGAGGCGATGGAGCGCTTCGCCGCCGACGCGCAGGCCTCCG
GGCGGCGCCTGGTGCTCTGCGGGGACCTGAACGTCGCGCGCACCGACCTGGACGTTCACCCGAAGGAGCGAAAGGCGGGC
GCCATCGGCCAGCTCCCGGAGGAGCGGGCGCTGTTCGAGCGCATCCTGTCGCGCGGCCTGGTGGACGTGGGGCGCGCGCT
CGATCCCGCCAACGAGGGGCTGTTCACCTGGTGGGCGCCGTGGCGCAACCTCCGCCAGCGGAACATCGGCTGGCGCATCG
ACTACGTGCTGGCGAGCGCGTCGCTCGCGGCCAGCGCCACGCGCTGCCGGGTGCTGGCCGACTTCGGCACGAGCGATCAC
GCGCCGGTGGTGGCCGAGTTCCCGGTGGCCGCGTCCCCGGCGGGCGCGGCGAACGGCGCGAGCGCGGCCTCGTAG

Upstream 100 bases:

>100_bases
GTCAGCCCCGCCCGGCCGCCCTCGGCCTCAGGTGGAGCGCGCCCGTGCCCGGTGACATCCGCCCGCCGGGCCCTTAGACA
GGAGGCCGGGAGGGGACCGC

Downstream 100 bases:

>100_bases
GCGGCGAGGAGCGGCGGCGGCACCGCCACGCGGCGGTGCTCCACCATCGCCTCGAGCATCTTCGCGTTGGCGGGCGCCTG
GCCGCGGAAGTGGTTGTTCG

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKIGTWNVNGIRAREAQVGEFIAREGPDVVCLQEIKAPPSKVPPSLCELEGYWCLWHGETAYSGVALLVRKAIAPERPAF
VHPPFDLETRIATCELGGVTVASIYVPNGGKDLAAKLRFLEAMERFAADAQASGRRLVLCGDLNVARTDLDVHPKERKAG
AIGQLPEERALFERILSRGLVDVGRALDPANEGLFTWWAPWRNLRQRNIGWRIDYVLASASLAASATRCRVLADFGTSDH
APVVAEFPVAASPAGAANGASAAS

Sequences:

>Translated_264_residues
MKIGTWNVNGIRAREAQVGEFIAREGPDVVCLQEIKAPPSKVPPSLCELEGYWCLWHGETAYSGVALLVRKAIAPERPAF
VHPPFDLETRIATCELGGVTVASIYVPNGGKDLAAKLRFLEAMERFAADAQASGRRLVLCGDLNVARTDLDVHPKERKAG
AIGQLPEERALFERILSRGLVDVGRALDPANEGLFTWWAPWRNLRQRNIGWRIDYVLASASLAASATRCRVLADFGTSDH
APVVAEFPVAASPAGAANGASAAS
>Mature_264_residues
MKIGTWNVNGIRAREAQVGEFIAREGPDVVCLQEIKAPPSKVPPSLCELEGYWCLWHGETAYSGVALLVRKAIAPERPAF
VHPPFDLETRIATCELGGVTVASIYVPNGGKDLAAKLRFLEAMERFAADAQASGRRLVLCGDLNVARTDLDVHPKERKAG
AIGQLPEERALFERILSRGLVDVGRALDPANEGLFTWWAPWRNLRQRNIGWRIDYVLASASLAASATRCRVLADFGTSDH
APVVAEFPVAASPAGAANGASAAS

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=254, Percent_Identity=36.2204724409449, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI18375503, Length=254, Percent_Identity=36.2204724409449, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI18375501, Length=254, Percent_Identity=36.2204724409449, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=31.5789473684211, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=30.8880308880309, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI221330655, Length=261, Percent_Identity=32.5670498084291, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17136678, Length=259, Percent_Identity=32.8185328185328, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28467; Mature: 28467

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGTWNVNGIRAREAQVGEFIAREGPDVVCLQEIKAPPSKVPPSLCELEGYWCLWHGET
CCEEECCCCCEEEHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCHHHCCCCEEEEEECCC
AYSGVALLVRKAIAPERPAFVHPPFDLETRIATCELGGVTVASIYVPNGGKDLAAKLRFL
HHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCEEEEEEEECCCCHHHHHHHHHH
EAMERFAADAQASGRRLVLCGDLNVARTDLDVHPKERKAGAIGQLPEERALFERILSRGL
HHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHH
VDVGRALDPANEGLFTWWAPWRNLRQRNIGWRIDYVLASASLAASATRCRVLADFGTSDH
HHHHHHCCCCCCCEEEEEHHHHHHHHCCCCEEEEHEEHHHHHHHHHHHEEEEEECCCCCC
APVVAEFPVAASPAGAANGASAAS
CCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIGTWNVNGIRAREAQVGEFIAREGPDVVCLQEIKAPPSKVPPSLCELEGYWCLWHGET
CCEEECCCCCEEEHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCHHHCCCCEEEEEECCC
AYSGVALLVRKAIAPERPAFVHPPFDLETRIATCELGGVTVASIYVPNGGKDLAAKLRFL
HHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCEEEEEEEECCCCHHHHHHHHHH
EAMERFAADAQASGRRLVLCGDLNVARTDLDVHPKERKAGAIGQLPEERALFERILSRGL
HHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHH
VDVGRALDPANEGLFTWWAPWRNLRQRNIGWRIDYVLASASLAASATRCRVLADFGTSDH
HHHHHHCCCCCCCEEEEEHHHHHHHHCCCCEEEEHEEHHHHHHHHHHHEEEEEECCCCCC
APVVAEFPVAASPAGAANGASAAS
CCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]