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The map label for this gene is cofH2 [H]

Identifier: 220915520

GI number: 220915520

Start: 451445

End: 452611

Strand: Reverse

Name: cofH2 [H]

Synonym: A2cp1_0401

Alternate gene names: 220915520

Gene position: 452611-451445 (Counterclockwise)

Preceding gene: 220915521

Following gene: 220915519

Centisome position: 9.0

GC content: 70.18

Gene sequence:

>1167_bases
ATGCTCTCGATCCTGGCGAACCGCGCGCTCGAGCGCGACGGCCTCGGGCCCATCCGCGACAAGGTGCTGGCCGGCGAGCG
GCTCACCGACGCCGACGGCCTGAGGCTGTTCGAGGCCGCCGACCTGCCCGCGCTGGGCGCGCTCGCGAACCACGTGCGCG
AGGCCCGCCACGGCGCGCTCGCGTTCTACAACCGCAACGTCCACCTCAACCCGACCAACGTCTGCGTCGCGTCCTGCAAG
TTCTGCTCGTTCGCGCGCAAGGACGACCAGCGCGCCTCCGACGGCTACACCATGTCGATCGACGAGGCGGTCGGGAAGGT
GCTGTCGCGCAAGCCGCTCGGCGTCACCGAGGTGCACATCGTCTCCGGGCTGCACCCGGACCTGCCGTTCGAGTACTACA
CCGAGCTGCTCTCGCGCATCCGCGCCGGCTGGCCCGAGGTGGCCATCAAGGCGTTCACCGCCATCGAGATCCACTTCTTC
GCCGAGAAGTTCGGCATGAGCTACGAGCAGGTGCTGCGCCGGCTGATGGACGCCGGCCTGCAGACCATGCCCGGCGGCGG
CGCCGAGATCTTCGCGCCGCGCGTGCGCCGGAAGATCTGCGACGACAAGGCCACCGCAGACCAGTGGATCGAGATCCACC
GCACCGCGCATCGCCTGGGCGTGAAGACGAACTCCACCATGCTCTACGGCCACATCGAGCGGCTGGACGAGCGGGTGGAC
CACATGCGCCGGCTGCGGGAGCTGCAGGACGAGACCCACGGCTTCCAGGTCTTCATCCCGCTCGCGTTCCACCCCGAGCA
CAACATGATCGGCAAGGCGTTCCCGAAGCCCACCGGCTACGACGCGCTGCGCACGCTCGCGGTGGCGCGGCTGTACCTCG
ACAACTTCGACCACGTGAAGGCGTACTGGGTCTCGCTCGGCGAGCGGCTGGCGCAGACCGCGCTGTCGTTCGGCGTGGAC
GACCTCGACGGCACCGTGCTGGAGGAGCGCATCTACCACATGGCCGGCTCGACGGTGCCGCAGGCGCTCAGCGAGCGTAC
GCTGCACCAGCTCATCCGCGCCGCCGGCCGCGTACCGGCCGAGCGCGACAGCCTCTACCGCGTGCTGAAGGTCCACGACG
CGCCGCTCCCCGAGGAGCCGCCCGCGCGCCTCCAGGTCACCGCCTGA

Upstream 100 bases:

>100_bases
CGGGGGGATGAAGCCCCCCCGGACCCCCCGCTCGCTCCGGGCCAGGCTCATCCGCCTGACCCTGCGCTCGCCGACCTGGA
TGACCCGGAGGAACCCTGAC

Downstream 100 bases:

>100_bases
TCCCGAGAGCTCCCATGCCGAAGAAGATCCGCGCCGCCGCCGTCTCGTTCCTGAACGCCTGGCCGCTCACCGCCGGGCTG
GAGGGCTCCGAGCGCATCGA

Product: Radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 2 [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MLSILANRALERDGLGPIRDKVLAGERLTDADGLRLFEAADLPALGALANHVREARHGALAFYNRNVHLNPTNVCVASCK
FCSFARKDDQRASDGYTMSIDEAVGKVLSRKPLGVTEVHIVSGLHPDLPFEYYTELLSRIRAGWPEVAIKAFTAIEIHFF
AEKFGMSYEQVLRRLMDAGLQTMPGGGAEIFAPRVRRKICDDKATADQWIEIHRTAHRLGVKTNSTMLYGHIERLDERVD
HMRRLRELQDETHGFQVFIPLAFHPEHNMIGKAFPKPTGYDALRTLAVARLYLDNFDHVKAYWVSLGERLAQTALSFGVD
DLDGTVLEERIYHMAGSTVPQALSERTLHQLIRAAGRVPAERDSLYRVLKVHDAPLPEEPPARLQVTA

Sequences:

>Translated_388_residues
MLSILANRALERDGLGPIRDKVLAGERLTDADGLRLFEAADLPALGALANHVREARHGALAFYNRNVHLNPTNVCVASCK
FCSFARKDDQRASDGYTMSIDEAVGKVLSRKPLGVTEVHIVSGLHPDLPFEYYTELLSRIRAGWPEVAIKAFTAIEIHFF
AEKFGMSYEQVLRRLMDAGLQTMPGGGAEIFAPRVRRKICDDKATADQWIEIHRTAHRLGVKTNSTMLYGHIERLDERVD
HMRRLRELQDETHGFQVFIPLAFHPEHNMIGKAFPKPTGYDALRTLAVARLYLDNFDHVKAYWVSLGERLAQTALSFGVD
DLDGTVLEERIYHMAGSTVPQALSERTLHQLIRAAGRVPAERDSLYRVLKVHDAPLPEEPPARLQVTA
>Mature_388_residues
MLSILANRALERDGLGPIRDKVLAGERLTDADGLRLFEAADLPALGALANHVREARHGALAFYNRNVHLNPTNVCVASCK
FCSFARKDDQRASDGYTMSIDEAVGKVLSRKPLGVTEVHIVSGLHPDLPFEYYTELLSRIRAGWPEVAIKAFTAIEIHFF
AEKFGMSYEQVLRRLMDAGLQTMPGGGAEIFAPRVRRKICDDKATADQWIEIHRTAHRLGVKTNSTMLYGHIERLDERVD
HMRRLRELQDETHGFQVFIPLAFHPEHNMIGKAFPKPTGYDALRTLAVARLYLDNFDHVKAYWVSLGERLAQTALSFGVD
DLDGTVLEERIYHMAGSTVPQALSERTLHQLIRAAGRVPAERDSLYRVLKVHDAPLPEEPPARLQVTA

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR012314
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43547; Mature: 43547

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSILANRALERDGLGPIRDKVLAGERLTDADGLRLFEAADLPALGALANHVREARHGAL
CCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEHHCCCCHHHHHHHHHHHHHCCEE
AFYNRNVHLNPTNVCVASCKFCSFARKDDQRASDGYTMSIDEAVGKVLSRKPLGVTEVHI
EEEECEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHH
VSGLHPDLPFEYYTELLSRIRAGWPEVAIKAFTAIEIHFFAEKFGMSYEQVLRRLMDAGL
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
QTMPGGGAEIFAPRVRRKICDDKATADQWIEIHRTAHRLGVKTNSTMLYGHIERLDERVD
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHH
HMRRLRELQDETHGFQVFIPLAFHPEHNMIGKAFPKPTGYDALRTLAVARLYLDNFDHVK
HHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
AYWVSLGERLAQTALSFGVDDLDGTVLEERIYHMAGSTVPQALSERTLHQLIRAAGRVPA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
ERDSLYRVLKVHDAPLPEEPPARLQVTA
CHHHHHHHHHHCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MLSILANRALERDGLGPIRDKVLAGERLTDADGLRLFEAADLPALGALANHVREARHGAL
CCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEHHCCCCHHHHHHHHHHHHHCCEE
AFYNRNVHLNPTNVCVASCKFCSFARKDDQRASDGYTMSIDEAVGKVLSRKPLGVTEVHI
EEEECEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHHH
VSGLHPDLPFEYYTELLSRIRAGWPEVAIKAFTAIEIHFFAEKFGMSYEQVLRRLMDAGL
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
QTMPGGGAEIFAPRVRRKICDDKATADQWIEIHRTAHRLGVKTNSTMLYGHIERLDERVD
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHH
HMRRLRELQDETHGFQVFIPLAFHPEHNMIGKAFPKPTGYDALRTLAVARLYLDNFDHVK
HHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
AYWVSLGERLAQTALSFGVDDLDGTVLEERIYHMAGSTVPQALSERTLHQLIRAAGRVPA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
ERDSLYRVLKVHDAPLPEEPPARLQVTA
CHHHHHHHHHHCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]