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The map label for this gene is cofH [H]

Identifier: 220915519

GI number: 220915519

Start: 449550

End: 451430

Strand: Reverse

Name: cofH [H]

Synonym: A2cp1_0400

Alternate gene names: 220915519

Gene position: 451430-449550 (Counterclockwise)

Preceding gene: 220915520

Following gene: 220915518

Centisome position: 8.98

GC content: 71.82

Gene sequence:

>1881_bases
ATGCCGAAGAAGATCCGCGCCGCCGCCGTCTCGTTCCTGAACGCCTGGCCGCTCACCGCCGGGCTGGAGGGCTCCGAGCG
CATCGAGCTGGTGCTGGCCGAGCCGTCCCGCTGCGCGGCCATGCTGGAGGCGGGCGAGGTGGACCTGGCGCTCGTGTCGG
TCGCCGCGCTCACCAAGGGCCAGTACGACATCGTGCCCGGCATCGCCATCGGCGCCGACGGCCCGGTGCAGACGGTGGTG
CTCGCCGGCGAGCAGTCGCCGGCCATCTGGGACGAGGTGTTCCTCGACACCGCCTCCCGCACCTCGCACGTGCTCGCGAA
GCTGGTGCTCGACGCCATGGGCGTGCACCCGAAGTTCACCCCCATGCACGCGGACGAGGGACTGGCCCGCGCCAGGGGCA
CCAAGGGCGCGCTCGTGATCGGCGACCGCGGGTTCGGCGTCCGCGCCAACCACGTGCTCGACCTCGGGCGCGAGTGGACC
CGGCTCATCGGGCTCCCCATGATCTTCGCGCTGTGGGCGGCGCGGCCGGGCGCGGTCACCCCCGAGGACGTGCAGGAGCT
GACCCGGGCTGCGCAGCACGGCCTCGGGATCCGCACCGAGCTGGCGCAGAAGTTCGCCGCGCAGAAGGGCGGCGACCCGG
AGAAGTACCGCCGCTACCTCACCCAGAAGATCCGCTACGGCCTCGGCCCCTACGAGCTGCAGGGGCTGGAGGCGTTCCTG
GAGCGCGCCGCCGCGAAGGGCTTCCTGCCCCCCATGCCGCTCCGCTTCGTGGACGACGTGGTGCGGACCCGGCGCGTGCG
CCGCGCCGTCTCGGTGGACACCGCGCTGCAGAAGGGCGCGGACGGCGAGCGGCTCGACGCCGACGAGGCGGCCACGCTCG
ACGAGAAGGCGCCGCTGCTCGAGCTCGGGCTGGCGGCCGACCAGCGCCGCCGCGCGCTCCACCCCGGCGACCTCGTCACC
TACATCGTGTCGCGGAACGTGAACTACACGAACGTGTGCACCACCGCGTGCCACTTCTGCGCGTTCTACCGCCCCCGCGG
CCACCAGGAGTCGTACGTCCTCACGCGCGAGGAGCTGACGCAGAAGATCGACGAGACCGTGGCGCTCGGCGGCATCGAGA
TCCTGCTGCAGGGCGGCCTCCACCCCGACCTCGGCGTCGAGTGGTACGAGGACCTGTTCCGCTGGGTGAAGCAGACCTGG
CCGGTGATCAACCTCCACGCGCTCTCCCCCGAGGAGATCTGGCACATCGCCCGGACGAGCGAGCTCGGGCTCGAGGCCAC
CATCGACCGGCTGGTCGCGGCCGGGATGGACTCGATCCCGGGCGGCGGCGCCGAGATCCTGGACGACGAGGTCCGCCGCC
GGATCGCGCCGCTCAAGTGCTCCACCGACGAGTGGCTCACCGTGATGAAGGCGGCCCACCTGAAGGGGCTGAAGAGCACC
GCCACCATGATGTTCGGGGTGGGCGAGGGGCCGGAGCACCGGGTGGCGCACCTCATGCGGCTGCGCGAGCTGCAGGACGA
GACGCACGGCTTCACCGCGTTCATCTGCTGGCCGTTCCAGTCGCAGAACACCCGGCTCGAGCCGGGCGACGGCAGCGCCC
ACGCCTACCTGCGCACGAACGCGCTCTCGCGCCTGGTGCTCGACAACGTCCCGAACCTGCAGGCGTCGTGGGTGACGATG
GGCGGGGGCGTGGCCCAGGCCGCGCTGCACATGGGCTGCAACGACTTCGGCCAGGTGATGATCGAGGAGAACGTGGTCTC
CGCGGCCGGCACCACCTTCAAGATGGACTCGGAGGAGGTGGAGCGGCACATCCGCGACGCCGGCTTCCGCGCCGCCCGCC
GCAACATGAAGTACCAGCTGGTGGAGGAGACCCGCGCGTGA

Upstream 100 bases:

>100_bases
AGCGCGACAGCCTCTACCGCGTGCTGAAGGTCCACGACGCGCCGCTCCCCGAGGAGCCGCCCGCGCGCCTCCAGGTCACC
GCCTGATCCCGAGAGCTCCC

Downstream 100 bases:

>100_bases
TCCGGGTGGTGGCCGCGCCCTGGGTGCTCCCCGGCCTCGCGCCCGGCGCACCGGCGGCGTCCGCGCCGGCGATCGCCGAC
GGCGCGGTGGCGCTGGAGGG

Product: Radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MPKKIRAAAVSFLNAWPLTAGLEGSERIELVLAEPSRCAAMLEAGEVDLALVSVAALTKGQYDIVPGIAIGADGPVQTVV
LAGEQSPAIWDEVFLDTASRTSHVLAKLVLDAMGVHPKFTPMHADEGLARARGTKGALVIGDRGFGVRANHVLDLGREWT
RLIGLPMIFALWAARPGAVTPEDVQELTRAAQHGLGIRTELAQKFAAQKGGDPEKYRRYLTQKIRYGLGPYELQGLEAFL
ERAAAKGFLPPMPLRFVDDVVRTRRVRRAVSVDTALQKGADGERLDADEAATLDEKAPLLELGLAADQRRRALHPGDLVT
YIVSRNVNYTNVCTTACHFCAFYRPRGHQESYVLTREELTQKIDETVALGGIEILLQGGLHPDLGVEWYEDLFRWVKQTW
PVINLHALSPEEIWHIARTSELGLEATIDRLVAAGMDSIPGGGAEILDDEVRRRIAPLKCSTDEWLTVMKAAHLKGLKST
ATMMFGVGEGPEHRVAHLMRLRELQDETHGFTAFICWPFQSQNTRLEPGDGSAHAYLRTNALSRLVLDNVPNLQASWVTM
GGGVAQAALHMGCNDFGQVMIEENVVSAAGTTFKMDSEEVERHIRDAGFRAARRNMKYQLVEETRA

Sequences:

>Translated_626_residues
MPKKIRAAAVSFLNAWPLTAGLEGSERIELVLAEPSRCAAMLEAGEVDLALVSVAALTKGQYDIVPGIAIGADGPVQTVV
LAGEQSPAIWDEVFLDTASRTSHVLAKLVLDAMGVHPKFTPMHADEGLARARGTKGALVIGDRGFGVRANHVLDLGREWT
RLIGLPMIFALWAARPGAVTPEDVQELTRAAQHGLGIRTELAQKFAAQKGGDPEKYRRYLTQKIRYGLGPYELQGLEAFL
ERAAAKGFLPPMPLRFVDDVVRTRRVRRAVSVDTALQKGADGERLDADEAATLDEKAPLLELGLAADQRRRALHPGDLVT
YIVSRNVNYTNVCTTACHFCAFYRPRGHQESYVLTREELTQKIDETVALGGIEILLQGGLHPDLGVEWYEDLFRWVKQTW
PVINLHALSPEEIWHIARTSELGLEATIDRLVAAGMDSIPGGGAEILDDEVRRRIAPLKCSTDEWLTVMKAAHLKGLKST
ATMMFGVGEGPEHRVAHLMRLRELQDETHGFTAFICWPFQSQNTRLEPGDGSAHAYLRTNALSRLVLDNVPNLQASWVTM
GGGVAQAALHMGCNDFGQVMIEENVVSAAGTTFKMDSEEVERHIRDAGFRAARRNMKYQLVEETRA
>Mature_625_residues
PKKIRAAAVSFLNAWPLTAGLEGSERIELVLAEPSRCAAMLEAGEVDLALVSVAALTKGQYDIVPGIAIGADGPVQTVVL
AGEQSPAIWDEVFLDTASRTSHVLAKLVLDAMGVHPKFTPMHADEGLARARGTKGALVIGDRGFGVRANHVLDLGREWTR
LIGLPMIFALWAARPGAVTPEDVQELTRAAQHGLGIRTELAQKFAAQKGGDPEKYRRYLTQKIRYGLGPYELQGLEAFLE
RAAAKGFLPPMPLRFVDDVVRTRRVRRAVSVDTALQKGADGERLDADEAATLDEKAPLLELGLAADQRRRALHPGDLVTY
IVSRNVNYTNVCTTACHFCAFYRPRGHQESYVLTREELTQKIDETVALGGIEILLQGGLHPDLGVEWYEDLFRWVKQTWP
VINLHALSPEEIWHIARTSELGLEATIDRLVAAGMDSIPGGGAEILDDEVRRRIAPLKCSTDEWLTVMKAAHLKGLKSTA
TMMFGVGEGPEHRVAHLMRLRELQDETHGFTAFICWPFQSQNTRLEPGDGSAHAYLRTNALSRLVLDNVPNLQASWVTMG
GGVAQAALHMGCNDFGQVMIEENVVSAAGTTFKMDSEEVERHIRDAGFRAARRNMKYQLVEETRA

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 68794; Mature: 68662

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKIRAAAVSFLNAWPLTAGLEGSERIELVLAEPSRCAAMLEAGEVDLALVSVAALTKG
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHCCC
QYDIVPGIAIGADGPVQTVVLAGEQSPAIWDEVFLDTASRTSHVLAKLVLDAMGVHPKFT
CEEECCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PMHADEGLARARGTKGALVIGDRGFGVRANHVLDLGREWTRLIGLPMIFALWAARPGAVT
CCCCCCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PEDVQELTRAAQHGLGIRTELAQKFAAQKGGDPEKYRRYLTQKIRYGLGPYELQGLEAFL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ERAAAKGFLPPMPLRFVDDVVRTRRVRRAVSVDTALQKGADGERLDADEAATLDEKAPLL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHH
ELGLAADQRRRALHPGDLVTYIVSRNVNYTNVCTTACHFCAFYRPRGHQESYVLTREELT
HHCCCCHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHH
QKIDETVALGGIEILLQGGLHPDLGVEWYEDLFRWVKQTWPVINLHALSPEEIWHIARTS
HHHHHHHHHCCHHHEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHH
ELGLEATIDRLVAAGMDSIPGGGAEILDDEVRRRIAPLKCSTDEWLTVMKAAHLKGLKST
HCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHH
ATMMFGVGEGPEHRVAHLMRLRELQDETHGFTAFICWPFQSQNTRLEPGDGSAHAYLRTN
HHEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEHHH
ALSRLVLDNVPNLQASWVTMGGGVAQAALHMGCNDFGQVMIEENVVSAAGTTFKMDSEEV
HHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCHHHH
ERHIRDAGFRAARRNMKYQLVEETRA
HHHHHHHHHHHHHHCCCEEHHHHCCC
>Mature Secondary Structure 
PKKIRAAAVSFLNAWPLTAGLEGSERIELVLAEPSRCAAMLEAGEVDLALVSVAALTKG
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHCCC
QYDIVPGIAIGADGPVQTVVLAGEQSPAIWDEVFLDTASRTSHVLAKLVLDAMGVHPKFT
CEEECCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PMHADEGLARARGTKGALVIGDRGFGVRANHVLDLGREWTRLIGLPMIFALWAARPGAVT
CCCCCCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PEDVQELTRAAQHGLGIRTELAQKFAAQKGGDPEKYRRYLTQKIRYGLGPYELQGLEAFL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ERAAAKGFLPPMPLRFVDDVVRTRRVRRAVSVDTALQKGADGERLDADEAATLDEKAPLL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHH
ELGLAADQRRRALHPGDLVTYIVSRNVNYTNVCTTACHFCAFYRPRGHQESYVLTREELT
HHCCCCHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHH
QKIDETVALGGIEILLQGGLHPDLGVEWYEDLFRWVKQTWPVINLHALSPEEIWHIARTS
HHHHHHHHHCCHHHEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHH
ELGLEATIDRLVAAGMDSIPGGGAEILDDEVRRRIAPLKCSTDEWLTVMKAAHLKGLKST
HCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHH
ATMMFGVGEGPEHRVAHLMRLRELQDETHGFTAFICWPFQSQNTRLEPGDGSAHAYLRTN
HHEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEHHH
ALSRLVLDNVPNLQASWVTMGGGVAQAALHMGCNDFGQVMIEENVVSAAGTTFKMDSEEV
HHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCHHHH
ERHIRDAGFRAARRNMKYQLVEETRA
HHHHHHHHHHHHHHCCCEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]