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The map label for this gene is nadE [H]

Identifier: 220915428

GI number: 220915428

Start: 340968

End: 342677

Strand: Reverse

Name: nadE [H]

Synonym: A2cp1_0307

Alternate gene names: 220915428

Gene position: 342677-340968 (Counterclockwise)

Preceding gene: 220915432

Following gene: 220915427

Centisome position: 6.81

GC content: 75.2

Gene sequence:

>1710_bases
ATGGCCCGTTCCGGACGCATCGCGCTCGCGCAGGTGAACACGACGGTGGGGGACTTCGCCGGCAACGCCGCCAGGGTGCG
GGCCGCCGCCGAGGTCGCCCGGGAGGCCGGCGCCGCCCTCGCCGTCTTCCCCGAGCTGACGGTGTGCGGCTACCCGCCGC
GAGACCTGCTCGACCTGCCCGACTTCCTCGAGCGCGCCCGGCAGGCGCTGGAGGAGCTCGCCCGCCCGGCCGCCTGGTCG
AAGGGCATCGCGCTGGTGGTGGGATTCCCGGAGGCGCCCGACGGCGCGCCGCCGCCGGGCGTCTACAACGCCGCCGCGCT
GATCTCGGACGGCCGGGTGGTGGCGGTGGGGCGGAAGTCGCTCCTGCCCACCTACGACGTCTTCGACGAGACCCGCTACT
TCCTCCCGGCCGGCGCCTCCACCACCGCCGCCGCGCCCGAGGGGCTGGGCGTGCCGCTCGGGCTGTCGGTCTGCGAGGAC
ATCTGGAACGACCAGCGCTTCTGGGAGCGCCCGCGCTACGCGCGCGATCCCATCGCCGACCTGGTGCGCGCCGGGGCCGG
GCTGGTGGTGAACGTCTCCGCCTCGCCCTACGCCATGGGCAAGGCGCCGCTCCGCGAGCGCATGCTGTCCGCCAGCGCGC
GGGACCACGGGGCGCCCATCGCCTACGTGAACCAGGTGGGCGGGAACGACGCCCTGCTGTTCGACGGCGGGTCGATGCTC
CTCGCGCGCGACGGCGCGGTGCTGGCACGGGCGCCGCTGTTCGAGGAGGCGGTGCTGGTCTGCGATCTCGAGGGCGGCGC
GCCGCTCGCGCTGGGCCTCGACGGCCGCCCGCTGCCGCCGCCCCCGCCCCCGCCCGCGGATGCGCAGGCGGACGAGGTGC
TCCGGGCGCTGGTGATGGGCGTCCGCGACTACGTGCGCAAGTGCGGGTTCCGGCAGGCGGTGGTGGGCCTCTCCGGCGGC
ATCGACTCGGCGCTCACCGCCTGCATCGCCGCCGAGGCGCTCGGCGCCGAGAACGTCCTCGGGGTGGCGATGCCCTCCCG
CTACAGCTCCGGCCACTCGCGCGAGGACGCGGCCGAGCTGGCCCGCAACCTCGGCGTCCGCTTCCGCGAGATCGGCATCG
AGCCGATGCACGCCGCCTTCCTCGCGGCGCTCGCCGCCGACGGGGCGCCGCCGCTCTGCGACCTCGCCGACCAGAACGTC
CAGGCCCGCGTCCGCGGCCAGATCCTGATGGCGATCTCGAACGACACCGGCGCGCTGGTGCTCACCACCGGCAACAAGAG
CGAGATCGCGGTCGGCTACTGCACGCTCTACGGCGACATGGCCGGCGGCCTCGCCGCCATCGGCGACGTGCCGAAGACGC
TGGTGTACCGGGTGGCCCGCGCCGCGAACGCGCGCGCCGGCCGGACGCTCGTGCCGGAGCGCACCTTCACCAAGCCGCCC
TCGGCCGAGCTGAAGCCGGGCCAGCTCGACCAGGACTCGCTGCCGCCGTACGACGTGCTCGACGACATCCTGCAGGCGTA
CGTGGAGGAGCGCCGGCCGCTCGAGGCCATCGTGGCGCGCGGGCACGACGAGGCCATCGTGCGGCGCGTGCTGCGCATGG
TGGTGCAGAGCGAGTACAAGCGCCGGCAGGCGGCGCCGGTGCTGAAGGTGAGCGAGAAGGCGTTCGGCGAGGGGCGCCGC
TTCCCCATCGCCCAGGGCTACCGCTACTGA

Upstream 100 bases:

>100_bases
CGCGCGGAGCGGCCGGCGCAGCGGCGCGAGGAGCGGCGCGGAGGCCTCGGCGACCAGGATGCGGCGCGGCACGATGGCTC
CTAATGTAGCATCCTCTCCC

Downstream 100 bases:

>100_bases
CGCGCGCGCACCGCGGCGGCGCCGCACCCACCTCCCCCCGCATCGTCACCGCGCGCGACGCTTCGTCGCGCGCCGGGCCC
GGCATACACTCGGCCGCCCG

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MARSGRIALAQVNTTVGDFAGNAARVRAAAEVAREAGAALAVFPELTVCGYPPRDLLDLPDFLERARQALEELARPAAWS
KGIALVVGFPEAPDGAPPPGVYNAAALISDGRVVAVGRKSLLPTYDVFDETRYFLPAGASTTAAAPEGLGVPLGLSVCED
IWNDQRFWERPRYARDPIADLVRAGAGLVVNVSASPYAMGKAPLRERMLSASARDHGAPIAYVNQVGGNDALLFDGGSML
LARDGAVLARAPLFEEAVLVCDLEGGAPLALGLDGRPLPPPPPPPADAQADEVLRALVMGVRDYVRKCGFRQAVVGLSGG
IDSALTACIAAEALGAENVLGVAMPSRYSSGHSREDAAELARNLGVRFREIGIEPMHAAFLAALAADGAPPLCDLADQNV
QARVRGQILMAISNDTGALVLTTGNKSEIAVGYCTLYGDMAGGLAAIGDVPKTLVYRVARAANARAGRTLVPERTFTKPP
SAELKPGQLDQDSLPPYDVLDDILQAYVEERRPLEAIVARGHDEAIVRRVLRMVVQSEYKRRQAAPVLKVSEKAFGEGRR
FPIAQGYRY

Sequences:

>Translated_569_residues
MARSGRIALAQVNTTVGDFAGNAARVRAAAEVAREAGAALAVFPELTVCGYPPRDLLDLPDFLERARQALEELARPAAWS
KGIALVVGFPEAPDGAPPPGVYNAAALISDGRVVAVGRKSLLPTYDVFDETRYFLPAGASTTAAAPEGLGVPLGLSVCED
IWNDQRFWERPRYARDPIADLVRAGAGLVVNVSASPYAMGKAPLRERMLSASARDHGAPIAYVNQVGGNDALLFDGGSML
LARDGAVLARAPLFEEAVLVCDLEGGAPLALGLDGRPLPPPPPPPADAQADEVLRALVMGVRDYVRKCGFRQAVVGLSGG
IDSALTACIAAEALGAENVLGVAMPSRYSSGHSREDAAELARNLGVRFREIGIEPMHAAFLAALAADGAPPLCDLADQNV
QARVRGQILMAISNDTGALVLTTGNKSEIAVGYCTLYGDMAGGLAAIGDVPKTLVYRVARAANARAGRTLVPERTFTKPP
SAELKPGQLDQDSLPPYDVLDDILQAYVEERRPLEAIVARGHDEAIVRRVLRMVVQSEYKRRQAAPVLKVSEKAFGEGRR
FPIAQGYRY
>Mature_568_residues
ARSGRIALAQVNTTVGDFAGNAARVRAAAEVAREAGAALAVFPELTVCGYPPRDLLDLPDFLERARQALEELARPAAWSK
GIALVVGFPEAPDGAPPPGVYNAAALISDGRVVAVGRKSLLPTYDVFDETRYFLPAGASTTAAAPEGLGVPLGLSVCEDI
WNDQRFWERPRYARDPIADLVRAGAGLVVNVSASPYAMGKAPLRERMLSASARDHGAPIAYVNQVGGNDALLFDGGSMLL
ARDGAVLARAPLFEEAVLVCDLEGGAPLALGLDGRPLPPPPPPPADAQADEVLRALVMGVRDYVRKCGFRQAVVGLSGGI
DSALTACIAAEALGAENVLGVAMPSRYSSGHSREDAAELARNLGVRFREIGIEPMHAAFLAALAADGAPPLCDLADQNVQ
ARVRGQILMAISNDTGALVLTTGNKSEIAVGYCTLYGDMAGGLAAIGDVPKTLVYRVARAANARAGRTLVPERTFTKPPS
AELKPGQLDQDSLPPYDVLDDILQAYVEERRPLEAIVARGHDEAIVRRVLRMVVQSEYKRRQAAPVLKVSEKAFGEGRRF
PIAQGYRY

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=602, Percent_Identity=23.5880398671096, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17538756, Length=593, Percent_Identity=23.2715008431703, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60210; Mature: 60079

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSGRIALAQVNTTVGDFAGNAARVRAAAEVAREAGAALAVFPELTVCGYPPRDLLDLP
CCCCCCEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCEECCCCCHHHCCCH
DFLERARQALEELARPAAWSKGIALVVGFPEAPDGAPPPGVYNAAALISDGRVVAVGRKS
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHEECCCEEEEECCCC
LLPTYDVFDETRYFLPAGASTTAAAPEGLGVPLGLSVCEDIWNDQRFWERPRYARDPIAD
CCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCHHHH
LVRAGAGLVVNVSASPYAMGKAPLRERMLSASARDHGAPIAYVNQVGGNDALLFDGGSML
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCEE
LARDGAVLARAPLFEEAVLVCDLEGGAPLALGLDGRPLPPPPPPPADAQADEVLRALVMG
EEECCCCEECCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VRDYVRKCGFRQAVVGLSGGIDSALTACIAAEALGAENVLGVAMPSRYSSGHSREDAAEL
HHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHH
ARNLGVRFREIGIEPMHAAFLAALAADGAPPLCDLADQNVQARVRGQILMAISNDTGALV
HHHCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHCCCCHHEEECEEEEEEEECCCCEEE
LTTGNKSEIAVGYCTLYGDMAGGLAAIGDVPKTLVYRVARAANARAGRTLVPERTFTKPP
EEECCCCCEEEEEEEEEHHHCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SAELKPGQLDQDSLPPYDVLDDILQAYVEERRPLEAIVARGHDEAIVRRVLRMVVQSEYK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
RRQAAPVLKVSEKAFGEGRRFPIAQGYRY
HHHCCCHHEECHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARSGRIALAQVNTTVGDFAGNAARVRAAAEVAREAGAALAVFPELTVCGYPPRDLLDLP
CCCCCEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCEECCCCCHHHCCCH
DFLERARQALEELARPAAWSKGIALVVGFPEAPDGAPPPGVYNAAALISDGRVVAVGRKS
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHEECCCEEEEECCCC
LLPTYDVFDETRYFLPAGASTTAAAPEGLGVPLGLSVCEDIWNDQRFWERPRYARDPIAD
CCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCHHHH
LVRAGAGLVVNVSASPYAMGKAPLRERMLSASARDHGAPIAYVNQVGGNDALLFDGGSML
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCEE
LARDGAVLARAPLFEEAVLVCDLEGGAPLALGLDGRPLPPPPPPPADAQADEVLRALVMG
EEECCCCEECCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VRDYVRKCGFRQAVVGLSGGIDSALTACIAAEALGAENVLGVAMPSRYSSGHSREDAAEL
HHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHH
ARNLGVRFREIGIEPMHAAFLAALAADGAPPLCDLADQNVQARVRGQILMAISNDTGALV
HHHCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHCCCCHHEEECEEEEEEEECCCCEEE
LTTGNKSEIAVGYCTLYGDMAGGLAAIGDVPKTLVYRVARAANARAGRTLVPERTFTKPP
EEECCCCCEEEEEEEEEHHHCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SAELKPGQLDQDSLPPYDVLDDILQAYVEERRPLEAIVARGHDEAIVRRVLRMVVQSEYK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
RRQAAPVLKVSEKAFGEGRRFPIAQGYRY
HHHCCCHHEECHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]