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The map label for this gene is rimK [H]

Identifier: 220915134

GI number: 220915134

Start: 13994

End: 14977

Strand: Reverse

Name: rimK [H]

Synonym: A2cp1_0011

Alternate gene names: 220915134

Gene position: 14977-13994 (Counterclockwise)

Preceding gene: 220915140

Following gene: 220915133

Centisome position: 0.3

GC content: 73.27

Gene sequence:

>984_bases
ATGCCCCCAGGCAAGAAGACCGCCCGCGCGAAGCGCGCCGCCGCCCGCCCGCGTCCGGACGGCGAGCTCGGCGTCGCCAT
CCTGTCGCGCAACCCGAGGCTCTACTCCACCCGCCGCCTGGTCGAGGCGGCGACCGCCCTCGGCCACGCCCCGCGCGTGC
TCGACACCCTGCGATGCAACATGGTGCTGGCGCGCGACCGCCCGCGGATCTTCTACCTGGGCGAGGAGGTGGTCGCGAGC
GACGTCCACGTGGTCATCCCCCGGATCGGCGCCTCCATCACCGGCTACGGCGTGGCGGTGGTGAACCAGTTCGACCTGAT
GGGCGTCCCCGTCCTCGCCGCGGCCACGCCCATCGGCCGCTCGCGCGACAAGCTGCGCGCGCTGCAGCTCCTCTCGCGCT
TCGGCATCGACATCCCGCGCACCGTGATGTGCCGTTACCGCGAGGAGGTGCCGGAGGCGGTGCAGATGGTGGGGGGCCTC
CCCTGCATCATCAAGCTCATCCAGGGCAGCCAGGGCGTCGGCGTGATGATCGCGAACACCGAGGTGGAGGTGCGCGGGCT
GCTCGACACGCTCTGGACGCTCGGCCAGGAGATCCTGCTGCAGGAGCTGGTGGCCGAGTCGAAGGGCCGGGACGTCCGCG
CGCTGGTGGTCGGCGACCGCGTGGTCGCGGCCATGCGGCGGACGGCGCGCGCCGGCGAGTTCCGCTCCAACATCCACCGC
GGCGGCGTGGCCGAGGCGCTCGAGCTCGGGCGCGAGTACGCCGAGACGGCGGTGAAGGCGGCCCGGGTGATGGGGCTCGA
GGTGGCGGGCGTGGACATGCTGGAGGCCCGCACCGGGCCGAAGATCATGGAGGTCAACAGCTCGCCGGGCTTCGAGGGGC
TGGAGGCCGCCACCGGCCTCGACATCGCCGCCCTGTACGTGCGGCACGCGGTGGACTTCGCGAGCACGCGGCAGTCGGGC
TACGCGAGCGGGCACCTCGCCTGA

Upstream 100 bases:

>100_bases
AAGGGTTCTAGCAGCCGAGGGGGGCGGGATCAACGAACGGTTTTCCGAGCGCCGCGCCGCGTTGCGGGGGCGGGGGAAGG
CCGCTAACCTTCCCGCGCCC

Downstream 100 bases:

>100_bases
CCTCACGCTGCGTCGTGTGGGCGTCGCACCCCGTCGCCGGGTCGCGTGACACGGGGGAGCAGGCAGCCGCCCGTCGCTTC
TGCGCGGCGCGCGGGCGCGA

Product: RimK family alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPPGKKTARAKRAAARPRPDGELGVAILSRNPRLYSTRRLVEAATALGHAPRVLDTLRCNMVLARDRPRIFYLGEEVVAS
DVHVVIPRIGASITGYGVAVVNQFDLMGVPVLAAATPIGRSRDKLRALQLLSRFGIDIPRTVMCRYREEVPEAVQMVGGL
PCIIKLIQGSQGVGVMIANTEVEVRGLLDTLWTLGQEILLQELVAESKGRDVRALVVGDRVVAAMRRTARAGEFRSNIHR
GGVAEALELGREYAETAVKAARVMGLEVAGVDMLEARTGPKIMEVNSSPGFEGLEAATGLDIAALYVRHAVDFASTRQSG
YASGHLA

Sequences:

>Translated_327_residues
MPPGKKTARAKRAAARPRPDGELGVAILSRNPRLYSTRRLVEAATALGHAPRVLDTLRCNMVLARDRPRIFYLGEEVVAS
DVHVVIPRIGASITGYGVAVVNQFDLMGVPVLAAATPIGRSRDKLRALQLLSRFGIDIPRTVMCRYREEVPEAVQMVGGL
PCIIKLIQGSQGVGVMIANTEVEVRGLLDTLWTLGQEILLQELVAESKGRDVRALVVGDRVVAAMRRTARAGEFRSNIHR
GGVAEALELGREYAETAVKAARVMGLEVAGVDMLEARTGPKIMEVNSSPGFEGLEAATGLDIAALYVRHAVDFASTRQSG
YASGHLA
>Mature_326_residues
PPGKKTARAKRAAARPRPDGELGVAILSRNPRLYSTRRLVEAATALGHAPRVLDTLRCNMVLARDRPRIFYLGEEVVASD
VHVVIPRIGASITGYGVAVVNQFDLMGVPVLAAATPIGRSRDKLRALQLLSRFGIDIPRTVMCRYREEVPEAVQMVGGLP
CIIKLIQGSQGVGVMIANTEVEVRGLLDTLWTLGQEILLQELVAESKGRDVRALVVGDRVVAAMRRTARAGEFRSNIHRG
GVAEALELGREYAETAVKAARVMGLEVAGVDMLEARTGPKIMEVNSSPGFEGLEAATGLDIAALYVRHAVDFASTRQSGY
ASGHLA

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=228, Percent_Identity=24.5614035087719, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI134254462, Length=242, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787076, Length=294, Percent_Identity=45.9183673469388, Blast_Score=256, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 35192; Mature: 35061

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPGKKTARAKRAAARPRPDGELGVAILSRNPRLYSTRRLVEAATALGHAPRVLDTLRCN
CCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHC
MVLARDRPRIFYLGEEVVASDVHVVIPRIGASITGYGVAVVNQFDLMGVPVLAAATPIGR
EEEECCCCEEEEECHHHHHCCCEEEEEHHCCCCCCCHHHEEEHHHHCCCCHHHHCCCCCC
SRDKLRALQLLSRFGIDIPRTVMCRYREEVPEAVQMVGGLPCIIKLIQGSQGVGVMIANT
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECC
EVEVRGLLDTLWTLGQEILLQELVAESKGRDVRALVVGDRVVAAMRRTARAGEFRSNIHR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHC
GGVAEALELGREYAETAVKAARVMGLEVAGVDMLEARTGPKIMEVNSSPGFEGLEAATGL
CCHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHCCCCCEEEECCCCCCCCHHHHCCC
DIAALYVRHAVDFASTRQSGYASGHLA
HHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PPGKKTARAKRAAARPRPDGELGVAILSRNPRLYSTRRLVEAATALGHAPRVLDTLRCN
CCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHC
MVLARDRPRIFYLGEEVVASDVHVVIPRIGASITGYGVAVVNQFDLMGVPVLAAATPIGR
EEEECCCCEEEEECHHHHHCCCEEEEEHHCCCCCCCHHHEEEHHHHCCCCHHHHCCCCCC
SRDKLRALQLLSRFGIDIPRTVMCRYREEVPEAVQMVGGLPCIIKLIQGSQGVGVMIANT
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECC
EVEVRGLLDTLWTLGQEILLQELVAESKGRDVRALVVGDRVVAAMRRTARAGEFRSNIHR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHC
GGVAEALELGREYAETAVKAARVMGLEVAGVDMLEARTGPKIMEVNSSPGFEGLEAATGL
CCHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHCCCCCEEEECCCCCCCCHHHHCCC
DIAALYVRHAVDFASTRQSGYASGHLA
HHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA