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The map label for this gene is rhtB [C]

Identifier: 220914941

GI number: 220914941

Start: 318714

End: 319391

Strand: Reverse

Name: rhtB [C]

Synonym: Mnod_8095

Alternate gene names: 220914941

Gene position: 319391-318714 (Counterclockwise)

Preceding gene: 220914942

Following gene: 220914934

Centisome position: 69.73

GC content: 67.85

Gene sequence:

>678_bases
GTGAACGTCGTCGGTGTCGTCACAGCCATGCCAGACCTGAGCCAACTCGCCCTGTACTTCGCCGCGGCCTTCGTGCTCGC
CGTCACGCCCGGTCCGGGCCTCTTCTACGTCGCTGCGCGCACGCTCGCGGGCGGTCGTGCGGAAGGCGTGGCCTCCAGCT
TCGGCACCGGGTTGGGCGGTATGGTCCACGTCCTCGCGGGCAGCTTGGGCGTGTCCGCCATCGTGCTCGCAAGCGCAGAA
TTATTCACCGCGCTGAAGCTGATCGGGGCTGCCTATCTCGTCTGGATCGGCCTTCGCACCTTCCAATCGGCCCGCCGGGA
GGCGTCGACAGCGTTGAACAGCGTGCCAGCGACACCGCCGATCGGACCGCTTCGGGCGTTCCGTGAAGGCGTGCTGGTCG
AGGCGCTGAACCCGAAGACAGCGGCCTTCTTCCTGGCCTTCGTTCCGCAGTTCGTGGACCCGGCCGGAGGCCATGTCGCG
CTGCAATTCGTGGTGCTCGGCTTCGTGTCGGTTGCGCTCAACACCCTCGCCGACATCGTGGTCGCCTTTGCGGCGAGCGG
CATCCGAGAAGGCGCAGCAGCACGTCCCGCATTCATCCGCCGCCTGCGAGAGGCGTCGGGAACCGCGATGATCGCCCTCG
GCATCGGCCTCGCTCTGGCGAAGCGCCCTGCGACCTGA

Upstream 100 bases:

>100_bases
TGAGCCAGAACCGGAACGCGACCGACCGTGCCGGCGTGGCGGCGGGTCTCGCGACGAGCGAGCGCGCATCCGACCGCGAG
GTCGCCCCTCTCATCCCGTC

Downstream 100 bases:

>100_bases
TTGCTGGGCAACGCTGCTCTCACACCAAGACCGAAGCTGCCATTTTGATTTCCTGGCACTCAATTCTTGCAGGTTTAAGT
GTCTGAAAAACCGTCGGATA

Product: Lysine exporter protein (LYSE/YGGA)

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MNVVGVVTAMPDLSQLALYFAAAFVLAVTPGPGLFYVAARTLAGGRAEGVASSFGTGLGGMVHVLAGSLGVSAIVLASAE
LFTALKLIGAAYLVWIGLRTFQSARREASTALNSVPATPPIGPLRAFREGVLVEALNPKTAAFFLAFVPQFVDPAGGHVA
LQFVVLGFVSVALNTLADIVVAFAASGIREGAAARPAFIRRLREASGTAMIALGIGLALAKRPAT

Sequences:

>Translated_225_residues
MNVVGVVTAMPDLSQLALYFAAAFVLAVTPGPGLFYVAARTLAGGRAEGVASSFGTGLGGMVHVLAGSLGVSAIVLASAE
LFTALKLIGAAYLVWIGLRTFQSARREASTALNSVPATPPIGPLRAFREGVLVEALNPKTAAFFLAFVPQFVDPAGGHVA
LQFVVLGFVSVALNTLADIVVAFAASGIREGAAARPAFIRRLREASGTAMIALGIGLALAKRPAT
>Mature_225_residues
MNVVGVVTAMPDLSQLALYFAAAFVLAVTPGPGLFYVAARTLAGGRAEGVASSFGTGLGGMVHVLAGSLGVSAIVLASAE
LFTALKLIGAAYLVWIGLRTFQSARREASTALNSVPATPPIGPLRAFREGVLVEALNPKTAAFFLAFVPQFVDPAGGHVA
LQFVVLGFVSVALNTLADIVVAFAASGIREGAAARPAFIRRLREASGTAMIALGIGLALAKRPAT

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=205, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786522, Length=119, Percent_Identity=30.2521008403361, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22919; Mature: 22919

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVGVVTAMPDLSQLALYFAAAFVLAVTPGPGLFYVAARTLAGGRAEGVASSFGTGLGG
CCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHH
MVHVLAGSLGVSAIVLASAELFTALKLIGAAYLVWIGLRTFQSARREASTALNSVPATPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGPLRAFREGVLVEALNPKTAAFFLAFVPQFVDPAGGHVALQFVVLGFVSVALNTLADIV
CHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VAFAASGIREGAAARPAFIRRLREASGTAMIALGIGLALAKRPAT
HHHHHHHHHCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVVGVVTAMPDLSQLALYFAAAFVLAVTPGPGLFYVAARTLAGGRAEGVASSFGTGLGG
CCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHH
MVHVLAGSLGVSAIVLASAELFTALKLIGAAYLVWIGLRTFQSARREASTALNSVPATPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGPLRAFREGVLVEALNPKTAAFFLAFVPQFVDPAGGHVALQFVVLGFVSVALNTLADIV
CHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VAFAASGIREGAAARPAFIRRLREASGTAMIALGIGLALAKRPAT
HHHHHHHHHCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]