Click here to switch to the map view.

The map label for this gene is arnB [H]

Identifier: 220905436

GI number: 220905436

Start: 2620160

End: 2621299

Strand: Reverse

Name: arnB [H]

Synonym: Ddes_2174

Alternate gene names: 220905436

Gene position: 2621299-2620160 (Counterclockwise)

Preceding gene: 220905437

Following gene: 220905435

Centisome position: 91.23

GC content: 60.35

Gene sequence:

>1140_bases
ATGTCCATGCGTCTTTCCCGTTCCATAGTGGGTGAAGCGGAAGCCCGTGCAGTGAGCCGTGTTCTTCTGGAAGACGGCTA
TCTCGGCATGGGCAATGAGGTGCGCCTTTTTGAAGAAGAAGTGGCGCAGTATCTTGGTGTGCGGCCTGATCAGGTCATCA
CCGTCAACACGGGCACAGCCGCCCTGCATCTGGCCGTGGACGCCGCGGCGGCCCTGAGTCGTGTACCCGGCAAACCCGAG
GTGCTGGTTCCCTCGTTGACATTTGTGGCTTCTTTTCAGGCCATCACTGCGGCGGGCTGTGTACCTGTAGCCTGCGACGT
ATTGGCCGAGACAGGTACGCTTGACCTGGCCGATGCGGAAAAGCGGCTTACCCCCAATACCGTTGCGGTCATGTTTGTGG
ACTATGCCAGCAATCCCTGGCATCTGGACGAAGTGTACGATTTTGCCCGGCGCAAAGGGCTGCGCGTGATTGAAGATGCG
GCCCATGCCTTTGGTTGCAAGCATCACGGGCGCAAGATCGGCAGCTTCGGCGATATGGTCTGCTTCAGCTTTGACGGCAT
CAAGAATATAACCTGCGGCGAAGGTGGCTGTCTCGTGGCTTTTGAGGCCGAAGCGGCGCACCTTGCCTCGGATGCCCGTC
TGCTTTCGGTAGAAAATGACGCCCAGAAACGTTTTGCCGGAGCACGCTCCTGGGATCCGGACGTGAAAAGGCAGGGCTGG
CGCTACCACATGAGCAATATCATGGCCGCCATCGGGCGTGTGCAGCTCTCGCGTCTGGAGGCGGAGTTTATTCCCGCACG
GCGCGCGCTGGCGGCCCGCTACGCCGAAAAGCTGGCCTCCGTGCCGGGCGTGGCCCTGCTGCATACCGATCCGCAGGATT
TTATCGTGCCGCATATAATGCCCGTGCGCATCCTGCATGGGCGCAAGGACGAGGTAAAAGACGCCCTGGCGGCCAGGAAC
CTGCCAACGGGCGTACACTATAAACCCAATCATCTGCTGAGCTGCTTCAGCACCGGGCAAAGCCTGCCCGTTACCGAACA
GCTCTATGCCGAACTGGTGACCCTGCCCATGCATCCCGGCCTGACCACTGAAGATGTGGACGCCATATGCGAGGGCGTGG
CCCAGGCGCTTGCGCGTTGA

Upstream 100 bases:

>100_bases
GCACCGCAGCGCGTCCGGCTTACAGGCACATTGCGCCGTGCCTCATGCCTTCTGGCTGTCAGGCCCGGCCGGGCCAATGC
CCGTGCAGCAGGAGGCTGTC

Downstream 100 bases:

>100_bases
GCGGGCTGAAGCCCGCACTGTCCTGAAGGAGGCTGTCTTGTCGTCCCGTCCTGTTCCTTTGCTCGCGCTGGTGGTTCCTT
GCTATAACGAGGAAGAAACC

Product: DegT/DnrJ/EryC1/StrS aminotransferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrH; UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase; UDP-Ara4O aminotransferase; UDP-4-amino-4-deoxy-L-arabinose aminotransferase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSMRLSRSIVGEAEARAVSRVLLEDGYLGMGNEVRLFEEEVAQYLGVRPDQVITVNTGTAALHLAVDAAAALSRVPGKPE
VLVPSLTFVASFQAITAAGCVPVACDVLAETGTLDLADAEKRLTPNTVAVMFVDYASNPWHLDEVYDFARRKGLRVIEDA
AHAFGCKHHGRKIGSFGDMVCFSFDGIKNITCGEGGCLVAFEAEAAHLASDARLLSVENDAQKRFAGARSWDPDVKRQGW
RYHMSNIMAAIGRVQLSRLEAEFIPARRALAARYAEKLASVPGVALLHTDPQDFIVPHIMPVRILHGRKDEVKDALAARN
LPTGVHYKPNHLLSCFSTGQSLPVTEQLYAELVTLPMHPGLTTEDVDAICEGVAQALAR

Sequences:

>Translated_379_residues
MSMRLSRSIVGEAEARAVSRVLLEDGYLGMGNEVRLFEEEVAQYLGVRPDQVITVNTGTAALHLAVDAAAALSRVPGKPE
VLVPSLTFVASFQAITAAGCVPVACDVLAETGTLDLADAEKRLTPNTVAVMFVDYASNPWHLDEVYDFARRKGLRVIEDA
AHAFGCKHHGRKIGSFGDMVCFSFDGIKNITCGEGGCLVAFEAEAAHLASDARLLSVENDAQKRFAGARSWDPDVKRQGW
RYHMSNIMAAIGRVQLSRLEAEFIPARRALAARYAEKLASVPGVALLHTDPQDFIVPHIMPVRILHGRKDEVKDALAARN
LPTGVHYKPNHLLSCFSTGQSLPVTEQLYAELVTLPMHPGLTTEDVDAICEGVAQALAR
>Mature_378_residues
SMRLSRSIVGEAEARAVSRVLLEDGYLGMGNEVRLFEEEVAQYLGVRPDQVITVNTGTAALHLAVDAAAALSRVPGKPEV
LVPSLTFVASFQAITAAGCVPVACDVLAETGTLDLADAEKRLTPNTVAVMFVDYASNPWHLDEVYDFARRKGLRVIEDAA
HAFGCKHHGRKIGSFGDMVCFSFDGIKNITCGEGGCLVAFEAEAAHLASDARLLSVENDAQKRFAGARSWDPDVKRQGWR
YHMSNIMAAIGRVQLSRLEAEFIPARRALAARYAEKLASVPGVALLHTDPQDFIVPHIMPVRILHGRKDEVKDALAARNL
PTGVHYKPNHLLSCFSTGQSLPVTEQLYAELVTLPMHPGLTTEDVDAICEGVAQALAR

Specific function: Catalyzes the conversion of UDP-4-keto-arabinose (UDP- Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family. ArnB subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=381, Percent_Identity=32.5459317585302, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI2367285, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022850
- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: =2.6.1.87 [H]

Molecular weight: Translated: 41080; Mature: 40949

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMRLSRSIVGEAEARAVSRVLLEDGYLGMGNEVRLFEEEVAQYLGVRPDQVITVNTGTA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCE
ALHLAVDAAAALSRVPGKPEVLVPSLTFVASFQAITAAGCVPVACDVLAETGTLDLADAE
EEEHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCHH
KRLTPNTVAVMFVDYASNPWHLDEVYDFARRKGLRVIEDAAHAFGCKHHGRKIGSFGDMV
HCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHCCCCCCCCCCEE
CFSFDGIKNITCGEGGCLVAFEAEAAHLASDARLLSVENDAQKRFAGARSWDPDVKRQGW
EEECCCCCCCCCCCCCEEEEEECCHHHHHCCCEEEEECCHHHHHHCCCCCCCCHHHHCCH
RYHMSNIMAAIGRVQLSRLEAEFIPARRALAARYAEKLASVPGVALLHTDPQDFIVPHIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHH
PVRILHGRKDEVKDALAARNLPTGVHYKPNHLLSCFSTGQSLPVTEQLYAELVTLPMHPG
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
LTTEDVDAICEGVAQALAR
CCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SMRLSRSIVGEAEARAVSRVLLEDGYLGMGNEVRLFEEEVAQYLGVRPDQVITVNTGTA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCE
ALHLAVDAAAALSRVPGKPEVLVPSLTFVASFQAITAAGCVPVACDVLAETGTLDLADAE
EEEHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCHH
KRLTPNTVAVMFVDYASNPWHLDEVYDFARRKGLRVIEDAAHAFGCKHHGRKIGSFGDMV
HCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHCCCCCCCCCCEE
CFSFDGIKNITCGEGGCLVAFEAEAAHLASDARLLSVENDAQKRFAGARSWDPDVKRQGW
EEECCCCCCCCCCCCCEEEEEECCHHHHHCCCEEEEECCHHHHHHCCCCCCCCHHHHCCH
RYHMSNIMAAIGRVQLSRLEAEFIPARRALAARYAEKLASVPGVALLHTDPQDFIVPHIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHH
PVRILHGRKDEVKDALAARNLPTGVHYKPNHLLSCFSTGQSLPVTEQLYAELVTLPMHPG
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
LTTEDVDAICEGVAQALAR
CCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA