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The map label for this gene is dnaB [H]

Identifier: 220905283

GI number: 220905283

Start: 2434210

End: 2435736

Strand: Reverse

Name: dnaB [H]

Synonym: Ddes_2021

Alternate gene names: 220905283

Gene position: 2435736-2434210 (Counterclockwise)

Preceding gene: 220905284

Following gene: 220905282

Centisome position: 84.77

GC content: 61.36

Gene sequence:

>1527_bases
GTGGTTTCCCACAGTGATCATGGCTCCGCCCCCGGCCCCGAGGCCGGGGGCGCGCCTTTTGGGCAGAACGGTGCTTCCGG
GCGCGGCGGTCGTGGCGGCCAGTCGGGTCAAGGTTCGTTCAAGGCCCGGCAGGGCAGTGCCGCAGACAGGGCCGAGGCGG
ATCTTTTGCGCCGCGTGCCGCCCCACAGTGTGGAGGCGGAGCAGGCTGTGCTCGGTGGCGTGCTCATGCGTCCCCAGCTC
ATGCACGCCATTGTGGACCAACTGGCTGCCGAAGATTTTTATGTGCCGACCCATGCCACAATTTACGGCGCCTTTCTTGA
ACTGTACCGCAAATCCGCTCCCATAGACCTGCTCTCCACAGCAGAAATGCTGAAAAGCCGCAACGCTCTGGAAGAAGCGG
GCGGGGCCGTATACCTTGGCGACCTTGCCCAGGCCGTAGTCTCCGGTGCCAACGCCGAATATTATGCTACCATCGTGCGC
GACAAGGCCCTGCAACGCGGTCTTATCGAAGCATGCTCCGGCATCATCACCAACTGCTATGACGCCTCTCTTGAGGTGGG
AACCCTGCTGGACGAATCCGAGCAGGCGGTTTTTGCCATTTCGCAGCGCACTTCCGGGCGCGATTTTACCCCCACCCGTG
AACTGCTGGACAGGGTTTTTGACAACCTTTCCCGCCTGGCCGACGCCAAGGACGTAATCACCGGCGTCACCACCGGCTAT
ACCCGGCTGGACAAGCTGACTGCCGGGCTTCAGCCTTCGGACCTCATCATTGTGGCGGCCCGCCCCAGTATGGGTAAAAC
GGCCTTCTCGATGTGCATGGCCCTGCACGCCGCCGTGCGCCAGAATGTTCCCGTGGCCGTCTTTTCGCTTGAAATGAGCA
AGGAACAGCTCATGCAGCGCATGCTGGCCGTGTGGGGCAAGGTGGACCTTTCCAAGCTGCGCCGTCCGTCGCTGCTCACC
GACGATGACTGGCAGCGCCTGTACGCCGCAGCCGACGTTGTGGCCCGCGCACCCATTTTTATTGACGATACCCCGGCTCT
GACCACGCTGGAGTTGCGCGCCCGCGCCCGGCGGCTCAAGGCGGACAAGGGGCTTGGCATGGTGGTGGTTGACTACCTGC
AGCTCATGCGCACCAGCCGCCGCACTGACTCGCGCGAACTGGAAATTTCAGATATTTCCCGTTCCCTTAAAGGGCTTGCC
AAGGAAATGCATGTGCCTGTGGTGGCCCTTTCGCAGTTGAACAGAAAGGTGGAAGAGCGCAGCGATAAACGCCCCATGCT
CTCCGACCTGCGCGAATCCGGCGCCATTGAGCAGGATGCGGACGTTATCATGTTTGTCTACCGGGACGACGTCTATAAAT
TCGTCAAACCCGCCGAGCGCCCTCCCCAGGGCGTGGCCGAAATCATTATCGGCAAGCAGCGTAACGGGCCTGTGGGGGTG
GCAGAGCTTATGTACATGTCGCCTTACACATCTTTTGAAGACATGGCTCCGGACTGGTCGCCGCCGCCTTCTGAAAGCGG
TTCATAG

Upstream 100 bases:

>100_bases
TGGTGCCGGTGAAGGTTGTGTCCGACCATCAGTCCTTTGAAGAAGAGCCTGCTCCTGAAGCTCCCGCTGAAGAAGCTGAG
GCCGCTGAATAGGGGATGTA

Downstream 100 bases:

>100_bases
TCGTTCCCGCAGCAGCGTGCCCTCCGGCACGCTGTTTTTTGTCTTTCACTGGTCTTCTCAGCCCTCGCACTGTTTCAGGC
CTTTCGTGCCAAATCATGAG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MVSHSDHGSAPGPEAGGAPFGQNGASGRGGRGGQSGQGSFKARQGSAADRAEADLLRRVPPHSVEAEQAVLGGVLMRPQL
MHAIVDQLAAEDFYVPTHATIYGAFLELYRKSAPIDLLSTAEMLKSRNALEEAGGAVYLGDLAQAVVSGANAEYYATIVR
DKALQRGLIEACSGIITNCYDASLEVGTLLDESEQAVFAISQRTSGRDFTPTRELLDRVFDNLSRLADAKDVITGVTTGY
TRLDKLTAGLQPSDLIIVAARPSMGKTAFSMCMALHAAVRQNVPVAVFSLEMSKEQLMQRMLAVWGKVDLSKLRRPSLLT
DDDWQRLYAAADVVARAPIFIDDTPALTTLELRARARRLKADKGLGMVVVDYLQLMRTSRRTDSRELEISDISRSLKGLA
KEMHVPVVALSQLNRKVEERSDKRPMLSDLRESGAIEQDADVIMFVYRDDVYKFVKPAERPPQGVAEIIIGKQRNGPVGV
AELMYMSPYTSFEDMAPDWSPPPSESGS

Sequences:

>Translated_508_residues
MVSHSDHGSAPGPEAGGAPFGQNGASGRGGRGGQSGQGSFKARQGSAADRAEADLLRRVPPHSVEAEQAVLGGVLMRPQL
MHAIVDQLAAEDFYVPTHATIYGAFLELYRKSAPIDLLSTAEMLKSRNALEEAGGAVYLGDLAQAVVSGANAEYYATIVR
DKALQRGLIEACSGIITNCYDASLEVGTLLDESEQAVFAISQRTSGRDFTPTRELLDRVFDNLSRLADAKDVITGVTTGY
TRLDKLTAGLQPSDLIIVAARPSMGKTAFSMCMALHAAVRQNVPVAVFSLEMSKEQLMQRMLAVWGKVDLSKLRRPSLLT
DDDWQRLYAAADVVARAPIFIDDTPALTTLELRARARRLKADKGLGMVVVDYLQLMRTSRRTDSRELEISDISRSLKGLA
KEMHVPVVALSQLNRKVEERSDKRPMLSDLRESGAIEQDADVIMFVYRDDVYKFVKPAERPPQGVAEIIIGKQRNGPVGV
AELMYMSPYTSFEDMAPDWSPPPSESGS
>Mature_508_residues
MVSHSDHGSAPGPEAGGAPFGQNGASGRGGRGGQSGQGSFKARQGSAADRAEADLLRRVPPHSVEAEQAVLGGVLMRPQL
MHAIVDQLAAEDFYVPTHATIYGAFLELYRKSAPIDLLSTAEMLKSRNALEEAGGAVYLGDLAQAVVSGANAEYYATIVR
DKALQRGLIEACSGIITNCYDASLEVGTLLDESEQAVFAISQRTSGRDFTPTRELLDRVFDNLSRLADAKDVITGVTTGY
TRLDKLTAGLQPSDLIIVAARPSMGKTAFSMCMALHAAVRQNVPVAVFSLEMSKEQLMQRMLAVWGKVDLSKLRRPSLLT
DDDWQRLYAAADVVARAPIFIDDTPALTTLELRARARRLKADKGLGMVVVDYLQLMRTSRRTDSRELEISDISRSLKGLA
KEMHVPVVALSQLNRKVEERSDKRPMLSDLRESGAIEQDADVIMFVYRDDVYKFVKPAERPPQGVAEIIIGKQRNGPVGV
AELMYMSPYTSFEDMAPDWSPPPSESGS

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=468, Percent_Identity=44.8717948717949, Blast_Score=384, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55261; Mature: 55261

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSHSDHGSAPGPEAGGAPFGQNGASGRGGRGGQSGQGSFKARQGSAADRAEADLLRRVP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PHSVEAEQAVLGGVLMRPQLMHAIVDQLAAEDFYVPTHATIYGAFLELYRKSAPIDLLST
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
AEMLKSRNALEEAGGAVYLGDLAQAVVSGANAEYYATIVRDKALQRGLIEACSGIITNCY
HHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DASLEVGTLLDESEQAVFAISQRTSGRDFTPTRELLDRVFDNLSRLADAKDVITGVTTGY
CCCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TRLDKLTAGLQPSDLIIVAARPSMGKTAFSMCMALHAAVRQNVPVAVFSLEMSKEQLMQR
HHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLAVWGKVDLSKLRRPSLLTDDDWQRLYAAADVVARAPIFIDDTPALTTLELRARARRLK
HHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHH
ADKGLGMVVVDYLQLMRTSRRTDSRELEISDISRSLKGLAKEMHVPVVALSQLNRKVEER
HCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SDKRPMLSDLRESGAIEQDADVIMFVYRDDVYKFVKPAERPPQGVAEIIIGKQRNGPVGV
CCCCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCH
AELMYMSPYTSFEDMAPDWSPPPSESGS
HHHHHHCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MVSHSDHGSAPGPEAGGAPFGQNGASGRGGRGGQSGQGSFKARQGSAADRAEADLLRRVP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PHSVEAEQAVLGGVLMRPQLMHAIVDQLAAEDFYVPTHATIYGAFLELYRKSAPIDLLST
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
AEMLKSRNALEEAGGAVYLGDLAQAVVSGANAEYYATIVRDKALQRGLIEACSGIITNCY
HHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DASLEVGTLLDESEQAVFAISQRTSGRDFTPTRELLDRVFDNLSRLADAKDVITGVTTGY
CCCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
TRLDKLTAGLQPSDLIIVAARPSMGKTAFSMCMALHAAVRQNVPVAVFSLEMSKEQLMQR
HHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLAVWGKVDLSKLRRPSLLTDDDWQRLYAAADVVARAPIFIDDTPALTTLELRARARRLK
HHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHH
ADKGLGMVVVDYLQLMRTSRRTDSRELEISDISRSLKGLAKEMHVPVVALSQLNRKVEER
HCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SDKRPMLSDLRESGAIEQDADVIMFVYRDDVYKFVKPAERPPQGVAEIIIGKQRNGPVGV
CCCCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCH
AELMYMSPYTSFEDMAPDWSPPPSESGS
HHHHHHCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]