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The map label for this gene is hemL

Identifier: 220905279

GI number: 220905279

Start: 2429531

End: 2430802

Strand: Reverse

Name: hemL

Synonym: Ddes_2017

Alternate gene names: 220905279

Gene position: 2430802-2429531 (Counterclockwise)

Preceding gene: 220905280

Following gene: 220905278

Centisome position: 84.6

GC content: 60.69

Gene sequence:

>1272_bases
ATGGACACTGTTTCGCGCCAGCTTTTTGAAAAAGCCCAGGCTGTCATACCCGGTGGCGTCAACAGCCCCGTGCGTGCCTG
CCACAATGTGGACAGCCAGCCTCTGTTTATTGCCGAGGCACACGGCTGTCATCTTACGGATGTGGACGGGCGGCAGTACA
TTGACTTTGTGCTTTCCTGGGGTCCTATGATTCTTGGGCATGACGAACCTTCCGTCACGCGTGCCGTTTGCGATGCCGCC
CATCGCGGCACCAGTTACGGCGCCCCCTGCCCTGATGAGGTGCTGCTGGCCGAGGCCGTGGTGGCCGCCATGCCCAGCCT
TGAGATGGTGCGTATGGTCAACTCGGGCACCGAGGCCACCATGAGCGCCCTGCGCCTGGCTCGCGCCGCCACCCGGCGTG
ACAAGGTTCTCAAATTTGTGGGCTGCTATCACGGACATGCCGATCCTTTTCTGGCTGCCGCCGGTTCGGGACTTGCCACG
TTTTCCATCCCCGGCACGCCGGGCGTACCCGCCGCCGTGGTGGCGGATACCCTGCTTGCCCCGTATAACGATCTGGAAGC
CGTCAAGGAATGCTTTGCCCGTCACGGCGAAAGCATTGCCGCCATTATTGTGGAGCCTGTGGCGGCCAATATGGGCCTTG
TGCTGCCCAAGCCCGGCTTTCTTGAAGGTTTGCGCGCCATATGCGACCAGTATGAAAGCCTGCTTATCTTTGACGAGGTC
ATCACGGGCTTTCGCGCGGCCTTCGGCGGGGCGCAGGCGCGCTTCAAGGTCGACCCCGACCTCACCACCTTCGGCAAGAT
CATCGGTGGCGGCCTGCCTGTGGGAGCCTTTGGCGGCAAACGCCGCTACATGGAACTGATCGCTCCCCGGGGCGGCGTGT
ACCAGGCGGGGACCCTTTCGGGCAATCCGCTGGCCATGGCTGCCGGTCTTGCCACACTGGGCATTCTCAGAAAGGCAGAC
TATGACGGCCTTGAAAACCGTACCCGCGCTTTTGCCTACAGCATGCGCGACATCATTGCCGCCAAGGGCGTACCACTGCA
GATGCCCACGCTGGCTTCCATGTTCTGCCCCTATTTCAGCGAACATGAAGTGACGGACTTTGCCGATGCGCAAAAGTGCG
ACCAAAAGCTCTTTACAAGTTTTTACAAGCAGATGCGCGCTCAGGGTATCTACCTTGCACCTTCGGGTTATGAGACCGGC
ATGGTGTCTTTCGCCCACACGGATGAAGACTTCAACCGCGCCCTGGATGCGGCCCGCAAGGTGATGTTCTAG

Upstream 100 bases:

>100_bases
GCCCCTGTTCACAGCGCGACGGCATTTACGGCCCGCGTAGGCGGGCAACATTGGCCGCCACGCAGCAGCCGCAAGCACAA
AATGTTATAAAGGAGCCAGA

Downstream 100 bases:

>100_bases
GGTCTGCTTTTATATGGCCGCTTTGCACAGAGCCGCTGTATACGGATAGGAAAAATGGGGTTTTGGCAACAGGAGGGTGC
GCGTATGCATTCACCTGAAG

Product: glutamate-1-semialdehyde-2,1-aminomutase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MDTVSRQLFEKAQAVIPGGVNSPVRACHNVDSQPLFIAEAHGCHLTDVDGRQYIDFVLSWGPMILGHDEPSVTRAVCDAA
HRGTSYGAPCPDEVLLAEAVVAAMPSLEMVRMVNSGTEATMSALRLARAATRRDKVLKFVGCYHGHADPFLAAAGSGLAT
FSIPGTPGVPAAVVADTLLAPYNDLEAVKECFARHGESIAAIIVEPVAANMGLVLPKPGFLEGLRAICDQYESLLIFDEV
ITGFRAAFGGAQARFKVDPDLTTFGKIIGGGLPVGAFGGKRRYMELIAPRGGVYQAGTLSGNPLAMAAGLATLGILRKAD
YDGLENRTRAFAYSMRDIIAAKGVPLQMPTLASMFCPYFSEHEVTDFADAQKCDQKLFTSFYKQMRAQGIYLAPSGYETG
MVSFAHTDEDFNRALDAARKVMF

Sequences:

>Translated_423_residues
MDTVSRQLFEKAQAVIPGGVNSPVRACHNVDSQPLFIAEAHGCHLTDVDGRQYIDFVLSWGPMILGHDEPSVTRAVCDAA
HRGTSYGAPCPDEVLLAEAVVAAMPSLEMVRMVNSGTEATMSALRLARAATRRDKVLKFVGCYHGHADPFLAAAGSGLAT
FSIPGTPGVPAAVVADTLLAPYNDLEAVKECFARHGESIAAIIVEPVAANMGLVLPKPGFLEGLRAICDQYESLLIFDEV
ITGFRAAFGGAQARFKVDPDLTTFGKIIGGGLPVGAFGGKRRYMELIAPRGGVYQAGTLSGNPLAMAAGLATLGILRKAD
YDGLENRTRAFAYSMRDIIAAKGVPLQMPTLASMFCPYFSEHEVTDFADAQKCDQKLFTSFYKQMRAQGIYLAPSGYETG
MVSFAHTDEDFNRALDAARKVMF
>Mature_423_residues
MDTVSRQLFEKAQAVIPGGVNSPVRACHNVDSQPLFIAEAHGCHLTDVDGRQYIDFVLSWGPMILGHDEPSVTRAVCDAA
HRGTSYGAPCPDEVLLAEAVVAAMPSLEMVRMVNSGTEATMSALRLARAATRRDKVLKFVGCYHGHADPFLAAAGSGLAT
FSIPGTPGVPAAVVADTLLAPYNDLEAVKECFARHGESIAAIIVEPVAANMGLVLPKPGFLEGLRAICDQYESLLIFDEV
ITGFRAAFGGAQARFKVDPDLTTFGKIIGGGLPVGAFGGKRRYMELIAPRGGVYQAGTLSGNPLAMAAGLATLGILRKAD
YDGLENRTRAFAYSMRDIIAAKGVPLQMPTLASMFCPYFSEHEVTDFADAQKCDQKLFTSFYKQMRAQGIYLAPSGYETG
MVSFAHTDEDFNRALDAARKVMF

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=297, Percent_Identity=29.2929292929293, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI13994255, Length=340, Percent_Identity=25.8823529411765, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI37574042, Length=315, Percent_Identity=26.984126984127, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI24119277, Length=303, Percent_Identity=26.4026402640264, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI226442705, Length=319, Percent_Identity=26.6457680250784, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI284507298, Length=211, Percent_Identity=30.8056872037915, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI226442709, Length=282, Percent_Identity=26.9503546099291, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1786349, Length=419, Percent_Identity=52.9832935560859, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1789016, Length=420, Percent_Identity=29.0476190476191, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI145693181, Length=288, Percent_Identity=31.25, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789759, Length=313, Percent_Identity=29.7124600638978, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788044, Length=320, Percent_Identity=30.3125, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787560, Length=412, Percent_Identity=29.3689320388349, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1786991, Length=313, Percent_Identity=29.7124600638978, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=327, Percent_Identity=29.6636085626911, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25144274, Length=316, Percent_Identity=27.8481012658228, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71992977, Length=335, Percent_Identity=24.4776119402985, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=322, Percent_Identity=26.7080745341615, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324432, Length=325, Percent_Identity=24.9230769230769, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357415, Length=321, Percent_Identity=26.1682242990654, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21356575, Length=320, Percent_Identity=27.1875, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_DESDA (B8J3A3)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480591.1
- ProteinModelPortal:   B8J3A3
- GeneID:   7285733
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_2017
- HOGENOM:   HBG725944
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45286; Mature: 45286

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVSRQLFEKAQAVIPGGVNSPVRACHNVDSQPLFIAEAHGCHLTDVDGRQYIDFVLSW
CCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHHHHHHC
GPMILGHDEPSVTRAVCDAAHRGTSYGAPCPDEVLLAEAVVAAMPSLEMVRMVNSGTEAT
CCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
MSALRLARAATRRDKVLKFVGCYHGHADPFLAAAGSGLATFSIPGTPGVPAAVVADTLLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHC
PYNDLEAVKECFARHGESIAAIIVEPVAANMGLVLPKPGFLEGLRAICDQYESLLIFDEV
CCHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
ITGFRAAFGGAQARFKVDPDLTTFGKIIGGGLPVGAFGGKRRYMELIAPRGGVYQAGTLS
HHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCC
GNPLAMAAGLATLGILRKADYDGLENRTRAFAYSMRDIIAAKGVPLQMPTLASMFCPYFS
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCC
EHEVTDFADAQKCDQKLFTSFYKQMRAQGIYLAPSGYETGMVSFAHTDEDFNRALDAARK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHH
VMF
HCC
>Mature Secondary Structure
MDTVSRQLFEKAQAVIPGGVNSPVRACHNVDSQPLFIAEAHGCHLTDVDGRQYIDFVLSW
CCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHHHHHHC
GPMILGHDEPSVTRAVCDAAHRGTSYGAPCPDEVLLAEAVVAAMPSLEMVRMVNSGTEAT
CCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
MSALRLARAATRRDKVLKFVGCYHGHADPFLAAAGSGLATFSIPGTPGVPAAVVADTLLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHC
PYNDLEAVKECFARHGESIAAIIVEPVAANMGLVLPKPGFLEGLRAICDQYESLLIFDEV
CCHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
ITGFRAAFGGAQARFKVDPDLTTFGKIIGGGLPVGAFGGKRRYMELIAPRGGVYQAGTLS
HHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCC
GNPLAMAAGLATLGILRKADYDGLENRTRAFAYSMRDIIAAKGVPLQMPTLASMFCPYFS
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCC
EHEVTDFADAQKCDQKLFTSFYKQMRAQGIYLAPSGYETGMVSFAHTDEDFNRALDAARK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHH
VMF
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA