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The map label for this gene is thiH [H]

Identifier: 220905208

GI number: 220905208

Start: 2345022

End: 2346413

Strand: Reverse

Name: thiH [H]

Synonym: Ddes_1946

Alternate gene names: 220905208

Gene position: 2346413-2345022 (Counterclockwise)

Preceding gene: 220905211

Following gene: 220905207

Centisome position: 81.66

GC content: 59.7

Gene sequence:

>1392_bases
ATGTCTGTGGAATCATTGCCCCGGTGGTTTAGTGTGGCTGCCATTGAGCGCGCCCTGGATCGTCAGGATGCCCCGGACGC
CATTGAGCTTCGTGATATTCTTGATAAGTCCATGCAGATGGTTCCGCTGGACGCAGATGAAATTGTGGCTCTTATGCGGG
TTGACGACCCCGTGGAGCATGAACGCATCCTCGCTGTTGCCGATGAGGTCAAACAGCGGGTTTACGGGGACCGCATGGTT
CTTTCCGCGCCGCTGCACCTTTCCAACCACTGCGGCAGCGAGTGCCTGTATTGCGCCAACCGCAGGGGAAACGGACAGAT
AGAACGCAAATACATGACATCGCCGGAAATGCGTGAAGCTGCGCTGCGCCTCATTCGTCAGGGGCACAAGCGTATTTTTC
TGGTCAGCGGGCAACTGCCCAACGCCGATGTGGAATATCTGGCCGAGGCCATCAGCATTCTGTACACGGTATTTGACGGT
GTGGGCGAAATCCACAGCGTCAATGTCAATGTGGGACCGCTTGAAAGCGCCCAGTACGAAACGCTGCTTGACGCCTATGT
GGGAACCGTCCTCATCTATCAGGACACCTATCATGAGGCCAGCTACCGCGCGGCGCATGTGTCCGGCCCCAAAAGCGATT
ACGTCAGGCGTCTTGAGGCTCCTGACACCGCCTTTGCGGCAGGGGTGCCTGATGTAGGCATGGGGCTTCTGCTCGGCCTC
GGCCCCTGGCGCTTTGACCTGCTGGCCCTCATCCAGCATGCGGCCCATCTGGAGCGGGTGTACGGCATGGGCTGTCGTAC
TGTAAGCCTGCACCGTATGCGTCCCGCCCCCGGCAGCCTTATGGAGGCTCCCTATCCGGTGAGCGATGCGGACTATCTGC
GCTGCGTGGCCCTTGCGCGGCTGGCCCTGCCCTATGCCGGCCTTATCCTGACCACCAGGGAACCGTCCGGCCTCTGGCGC
GACGGCTGTAATGCCGGTGGTTCGCAACTGCTTACGGGCAGCGTGGCAAATCCTTACGACGGCTGGTTTACCGCTTCAGG
CCAGCAGGTTCCTTTTCCCTGTGGTGAAGATTGCCATGTGGATGAGGTCGTGCGTTTTCTGCTTGAAGAGGCCCGGCATC
TGCCGTCCTTTTGCGCTGCCTGTCCACGCCTGGGCCGCAGGGGTGAAGAGTTCATTTCCATGGTGCGTGAATGCGGCATC
AAGGGGCAGTGCGGGCCGAACTCGGCGGCGTCGTTTATGGAATTTCTGCTGCATTATGCCACCCCCTATACCCGCATGAT
GGGCGAACGCCTTTTGAAGGAAAAGCTGGACAGGATGCCCATTCACGAGCGCGGCGCTGCAGATCGCCTGCTCAAGAAGG
TGCGGGCGGGCTGTATGGACGAGTTCATCTGA

Upstream 100 bases:

>100_bases
TAATGGACAATCAAATTACCGAATTTGAATGAGTATTTTGAAAGGCAAGATAGCTCTGCATTTTTTTACATAGCTCTTAA
CTTGTGCGAGGTTGACAAGC

Downstream 100 bases:

>100_bases
CGGCATTTCGCCGCAGACTTTGCGATTACTGCGGGCTGCCGCGCGACAGCCCGGGGCCTCTGGCCGAAGCGTCCAGCCAT
TTATGGCTGGACGCTTCACA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSVESLPRWFSVAAIERALDRQDAPDAIELRDILDKSMQMVPLDADEIVALMRVDDPVEHERILAVADEVKQRVYGDRMV
LSAPLHLSNHCGSECLYCANRRGNGQIERKYMTSPEMREAALRLIRQGHKRIFLVSGQLPNADVEYLAEAISILYTVFDG
VGEIHSVNVNVGPLESAQYETLLDAYVGTVLIYQDTYHEASYRAAHVSGPKSDYVRRLEAPDTAFAAGVPDVGMGLLLGL
GPWRFDLLALIQHAAHLERVYGMGCRTVSLHRMRPAPGSLMEAPYPVSDADYLRCVALARLALPYAGLILTTREPSGLWR
DGCNAGGSQLLTGSVANPYDGWFTASGQQVPFPCGEDCHVDEVVRFLLEEARHLPSFCAACPRLGRRGEEFISMVRECGI
KGQCGPNSAASFMEFLLHYATPYTRMMGERLLKEKLDRMPIHERGAADRLLKKVRAGCMDEFI

Sequences:

>Translated_463_residues
MSVESLPRWFSVAAIERALDRQDAPDAIELRDILDKSMQMVPLDADEIVALMRVDDPVEHERILAVADEVKQRVYGDRMV
LSAPLHLSNHCGSECLYCANRRGNGQIERKYMTSPEMREAALRLIRQGHKRIFLVSGQLPNADVEYLAEAISILYTVFDG
VGEIHSVNVNVGPLESAQYETLLDAYVGTVLIYQDTYHEASYRAAHVSGPKSDYVRRLEAPDTAFAAGVPDVGMGLLLGL
GPWRFDLLALIQHAAHLERVYGMGCRTVSLHRMRPAPGSLMEAPYPVSDADYLRCVALARLALPYAGLILTTREPSGLWR
DGCNAGGSQLLTGSVANPYDGWFTASGQQVPFPCGEDCHVDEVVRFLLEEARHLPSFCAACPRLGRRGEEFISMVRECGI
KGQCGPNSAASFMEFLLHYATPYTRMMGERLLKEKLDRMPIHERGAADRLLKKVRAGCMDEFI
>Mature_462_residues
SVESLPRWFSVAAIERALDRQDAPDAIELRDILDKSMQMVPLDADEIVALMRVDDPVEHERILAVADEVKQRVYGDRMVL
SAPLHLSNHCGSECLYCANRRGNGQIERKYMTSPEMREAALRLIRQGHKRIFLVSGQLPNADVEYLAEAISILYTVFDGV
GEIHSVNVNVGPLESAQYETLLDAYVGTVLIYQDTYHEASYRAAHVSGPKSDYVRRLEAPDTAFAAGVPDVGMGLLLGLG
PWRFDLLALIQHAAHLERVYGMGCRTVSLHRMRPAPGSLMEAPYPVSDADYLRCVALARLALPYAGLILTTREPSGLWRD
GCNAGGSQLLTGSVANPYDGWFTASGQQVPFPCGEDCHVDEVVRFLLEEARHLPSFCAACPRLGRRGEEFISMVRECGIK
GQCGPNSAASFMEFLLHYATPYTRMMGERLLKEKLDRMPIHERGAADRLLKKVRAGCMDEFI

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=290, Percent_Identity=28.2758620689655, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 51444; Mature: 51313

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVESLPRWFSVAAIERALDRQDAPDAIELRDILDKSMQMVPLDADEIVALMRVDDPVEH
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCHHH
ERILAVADEVKQRVYGDRMVLSAPLHLSNHCGSECLYCANRRGNGQIERKYMTSPEMREA
HHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCHHHEECCCCCCCCEEHHHCCCCHHHHH
ALRLIRQGHKRIFLVSGQLPNADVEYLAEAISILYTVFDGVGEIHSVNVNVGPLESAQYE
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
TLLDAYVGTVLIYQDTYHEASYRAAHVSGPKSDYVRRLEAPDTAFAAGVPDVGMGLLLGL
HHHHHHHCEEEEEECCHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHCCCCCHHHHHHHCC
GPWRFDLLALIQHAAHLERVYGMGCRTVSLHRMRPAPGSLMEAPYPVSDADYLRCVALAR
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LALPYAGLILTTREPSGLWRDGCNAGGSQLLTGSVANPYDGWFTASGQQVPFPCGEDCHV
HHHCCCCEEEEECCCCCCHHCCCCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCCCCCH
DEVVRFLLEEARHLPSFCAACPRLGRRGEEFISMVRECGIKGQCGPNSAASFMEFLLHYA
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
TPYTRMMGERLLKEKLDRMPIHERGAADRLLKKVRAGCMDEFI
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVESLPRWFSVAAIERALDRQDAPDAIELRDILDKSMQMVPLDADEIVALMRVDDPVEH
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCHHH
ERILAVADEVKQRVYGDRMVLSAPLHLSNHCGSECLYCANRRGNGQIERKYMTSPEMREA
HHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCHHHEECCCCCCCCEEHHHCCCCHHHHH
ALRLIRQGHKRIFLVSGQLPNADVEYLAEAISILYTVFDGVGEIHSVNVNVGPLESAQYE
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHH
TLLDAYVGTVLIYQDTYHEASYRAAHVSGPKSDYVRRLEAPDTAFAAGVPDVGMGLLLGL
HHHHHHHCEEEEEECCHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHCCCCCHHHHHHHCC
GPWRFDLLALIQHAAHLERVYGMGCRTVSLHRMRPAPGSLMEAPYPVSDADYLRCVALAR
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LALPYAGLILTTREPSGLWRDGCNAGGSQLLTGSVANPYDGWFTASGQQVPFPCGEDCHV
HHHCCCCEEEEECCCCCCHHCCCCCCCCCEEECCCCCCCCCCEECCCCCCCCCCCCCCCH
DEVVRFLLEEARHLPSFCAACPRLGRRGEEFISMVRECGIKGQCGPNSAASFMEFLLHYA
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
TPYTRMMGERLLKEKLDRMPIHERGAADRLLKKVRAGCMDEFI
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]