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The map label for this gene is fliF [H]

Identifier: 220904839

GI number: 220904839

Start: 1893496

End: 1895139

Strand: Reverse

Name: fliF [H]

Synonym: Ddes_1573

Alternate gene names: 220904839

Gene position: 1895139-1893496 (Counterclockwise)

Preceding gene: 220904840

Following gene: 220904838

Centisome position: 65.95

GC content: 57.3

Gene sequence:

>1644_bases
ATGCCTGCATTCATCCAACAGTTTATCAATTCTTTCAAAGCAGTATGGGCAAAGATGAGCCATCTGCAACGCATGGCCGC
CGCAGGCGGGGCCGTGGCTCTGCTGGCCGGTATTATTGGGCTTTCCATCTGGATGGGCCGCCCCGAGTACCGCGTTCTTT
ATTCCAATCTTGGCCCCGAAGACGCCAGCGTGGTGGTCAAGGCCCTGCAGTCCGACAAGGTGGCCTACCGCATTACTGAT
AACGGAGCCACCATCATGGTTCCGCAGGAAGTGGTATACGACCAGCGCATCAAGATCGCGGGCGAAGGCGGTCTGGTGGG
GCAGGGAATCGGTTTTGAAATTTTTGACAAGGTCAAGGTCGGTCAGACAGACTTTGTGCAGAAAATAAACTATACCCGCG
CCCTTCAGGGCGAACTGGCCCGCACGATCAGCGAATTTCCCAGTGTGGAAAGTGCGCGAGTGCATCTGGTCATCCCCCGC
CGCAGCCTTTTTGTCGAAGAGCGCCAGTCGCCTTCGGCTTCGGTGGTGCTCAAGCTTATTCGCCCCAACATCAAGGTTGA
CCAGAAAGAAATCAACGCCATCCTCAATATGATGCTCATGTCTGTGGAAGGCCTGGACAGGGGGCATGTTTCCATTACCG
ATACCAGCGGCAAGGTGCTCTACCAGCCGGAGGAAGACAGCCTGGCGGGCATTACCACCACGCAGATGGAATACCGTCAG
ACCGTGCAACGCAATCTTGAACGCCGTATCGAAGAAATGCTCCAGCCCCTTTTTGGCCCTGGCCGCGTCATTGCCAAGGT
CAACGCTGATATGGACTTCAGCCAGAAAACCATACGCCGCGAACTGTTCGACCCCGAAAGAACGGCTGTGCGCAGCGAAT
ACCGCAGCGAAGAAAGCCAGCAGGGGCGCGCCAACCTTGAGGCAGGCTCGCCAGATGTGAATTTTCGCGGCGACGGCATC
ACCGGTTCTGTCTCTGACCAGAGCGGAACCCGTGAAGCCCGCACCACCAACTACGAAATCAACAAGGAAGAACAGCAGAT
TGTGTCCAACGTGGGAGAATTGCGCCGCCTGACGGTTGCAGTCCTCATTGATGGCACGTATGTAAAGGCCGATGGCGCGT
GGACGTTTATGCCGCGCAAAAGCGAAGAGCTTGAACAGGTGCGTCAGCTTGTTCATAATGCCGTGGGGCTGGATACGGGC
CGGGGCGACGCGCTGGAGGTCAGCTCCGCGCCCTTTACCGACTCCGAGCCGCCCAGGGACCCGAATTTTGCCGACATGCT
GGCTGACTATGCCGAACGTCTGGGCAAGCCCCTGCTTAATGCCCTGCTGGCCTTCCTGTTCCTGATGCTTATAGTGCGGC
CTGTGGTGCTGGCTCTTATCCGGCCCAAGGTTGAGGCCGGTGAAATGGTGGAAGGCCTGGAAGGCCTGCCCGCCGCCGAA
GAGCAGCTCGCTCTGTACGAGGCGCTGGAAGAAGCCGCCAGAACAGAAGACGAGGTGCCGCAGGAAGACGACGAGGACGA
TCTGGTGTTCAAGGACATTGAAGCCCTGAAAGCGCACATCTTTACCCTGTCCGACAATCATATGGAACAGGTGGTTACCC
TGGTGCGCGGTTGGATGAAGAACGATGAAGCAGTTAAAGCCTAG

Upstream 100 bases:

>100_bases
TGTTATTACAATGCTATATGATTAGGCATGAATGTTGCTACGGCAAGGCAAGCCCGGCCTTGTGCCGGAAAACCGGCGCC
GGAGCGCACAGGAGCGGACC

Downstream 100 bases:

>100_bases
CCCCCCGCGGATGGGCCTTTCGTCAGGGCAGGGCGGCGGTTCTACGCCGGCCCTGTCACGGCTCAAGGCGCTGCGCCTGG
CGCAGAAAGAAACCAATCAG

Product: flagellar M-ring protein FliF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MPAFIQQFINSFKAVWAKMSHLQRMAAAGGAVALLAGIIGLSIWMGRPEYRVLYSNLGPEDASVVVKALQSDKVAYRITD
NGATIMVPQEVVYDQRIKIAGEGGLVGQGIGFEIFDKVKVGQTDFVQKINYTRALQGELARTISEFPSVESARVHLVIPR
RSLFVEERQSPSASVVLKLIRPNIKVDQKEINAILNMMLMSVEGLDRGHVSITDTSGKVLYQPEEDSLAGITTTQMEYRQ
TVQRNLERRIEEMLQPLFGPGRVIAKVNADMDFSQKTIRRELFDPERTAVRSEYRSEESQQGRANLEAGSPDVNFRGDGI
TGSVSDQSGTREARTTNYEINKEEQQIVSNVGELRRLTVAVLIDGTYVKADGAWTFMPRKSEELEQVRQLVHNAVGLDTG
RGDALEVSSAPFTDSEPPRDPNFADMLADYAERLGKPLLNALLAFLFLMLIVRPVVLALIRPKVEAGEMVEGLEGLPAAE
EQLALYEALEEAARTEDEVPQEDDEDDLVFKDIEALKAHIFTLSDNHMEQVVTLVRGWMKNDEAVKA

Sequences:

>Translated_547_residues
MPAFIQQFINSFKAVWAKMSHLQRMAAAGGAVALLAGIIGLSIWMGRPEYRVLYSNLGPEDASVVVKALQSDKVAYRITD
NGATIMVPQEVVYDQRIKIAGEGGLVGQGIGFEIFDKVKVGQTDFVQKINYTRALQGELARTISEFPSVESARVHLVIPR
RSLFVEERQSPSASVVLKLIRPNIKVDQKEINAILNMMLMSVEGLDRGHVSITDTSGKVLYQPEEDSLAGITTTQMEYRQ
TVQRNLERRIEEMLQPLFGPGRVIAKVNADMDFSQKTIRRELFDPERTAVRSEYRSEESQQGRANLEAGSPDVNFRGDGI
TGSVSDQSGTREARTTNYEINKEEQQIVSNVGELRRLTVAVLIDGTYVKADGAWTFMPRKSEELEQVRQLVHNAVGLDTG
RGDALEVSSAPFTDSEPPRDPNFADMLADYAERLGKPLLNALLAFLFLMLIVRPVVLALIRPKVEAGEMVEGLEGLPAAE
EQLALYEALEEAARTEDEVPQEDDEDDLVFKDIEALKAHIFTLSDNHMEQVVTLVRGWMKNDEAVKA
>Mature_546_residues
PAFIQQFINSFKAVWAKMSHLQRMAAAGGAVALLAGIIGLSIWMGRPEYRVLYSNLGPEDASVVVKALQSDKVAYRITDN
GATIMVPQEVVYDQRIKIAGEGGLVGQGIGFEIFDKVKVGQTDFVQKINYTRALQGELARTISEFPSVESARVHLVIPRR
SLFVEERQSPSASVVLKLIRPNIKVDQKEINAILNMMLMSVEGLDRGHVSITDTSGKVLYQPEEDSLAGITTTQMEYRQT
VQRNLERRIEEMLQPLFGPGRVIAKVNADMDFSQKTIRRELFDPERTAVRSEYRSEESQQGRANLEAGSPDVNFRGDGIT
GSVSDQSGTREARTTNYEINKEEQQIVSNVGELRRLTVAVLIDGTYVKADGAWTFMPRKSEELEQVRQLVHNAVGLDTGR
GDALEVSSAPFTDSEPPRDPNFADMLADYAERLGKPLLNALLAFLFLMLIVRPVVLALIRPKVEAGEMVEGLEGLPAAEE
QLALYEALEEAARTEDEVPQEDDEDDLVFKDIEALKAHIFTLSDNHMEQVVTLVRGWMKNDEAVKA

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=553, Percent_Identity=30.5605786618445, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 60825; Mature: 60694

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFIQQFINSFKAVWAKMSHLQRMAAAGGAVALLAGIIGLSIWMGRPEYRVLYSNLGPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
DASVVVKALQSDKVAYRITDNGATIMVPQEVVYDQRIKIAGEGGLVGQGIGFEIFDKVKV
HHHHHHHHHHCCCEEEEEECCCCEEEECHHHHHCCCEEEECCCCCEECCCCHHHHHHHCC
GQTDFVQKINYTRALQGELARTISEFPSVESARVHLVIPRRSLFVEERQSPSASVVLKLI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCCHHHHHHHHH
RPNIKVDQKEINAILNMMLMSVEGLDRGHVSITDTSGKVLYQPEEDSLAGITTTQMEYRQ
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHH
TVQRNLERRIEEMLQPLFGPGRVIAKVNADMDFSQKTIRRELFDPERTAVRSEYRSEESQ
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHH
QGRANLEAGSPDVNFRGDGITGSVSDQSGTREARTTNYEINKEEQQIVSNVGELRRLTVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEE
VLIDGTYVKADGAWTFMPRKSEELEQVRQLVHNAVGLDTGRGDALEVSSAPFTDSEPPRD
EEEECEEEEECCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCC
PNFADMLADYAERLGKPLLNALLAFLFLMLIVRPVVLALIRPKVEAGEMVEGLEGLPAAE
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHH
EQLALYEALEEAARTEDEVPQEDDEDDLVFKDIEALKAHIFTLSDNHMEQVVTLVRGWMK
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHC
NDEAVKA
CCCCCCC
>Mature Secondary Structure 
PAFIQQFINSFKAVWAKMSHLQRMAAAGGAVALLAGIIGLSIWMGRPEYRVLYSNLGPE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
DASVVVKALQSDKVAYRITDNGATIMVPQEVVYDQRIKIAGEGGLVGQGIGFEIFDKVKV
HHHHHHHHHHCCCEEEEEECCCCEEEECHHHHHCCCEEEECCCCCEECCCCHHHHHHHCC
GQTDFVQKINYTRALQGELARTISEFPSVESARVHLVIPRRSLFVEERQSPSASVVLKLI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCCHHHHHHHHH
RPNIKVDQKEINAILNMMLMSVEGLDRGHVSITDTSGKVLYQPEEDSLAGITTTQMEYRQ
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHH
TVQRNLERRIEEMLQPLFGPGRVIAKVNADMDFSQKTIRRELFDPERTAVRSEYRSEESQ
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHH
QGRANLEAGSPDVNFRGDGITGSVSDQSGTREARTTNYEINKEEQQIVSNVGELRRLTVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEE
VLIDGTYVKADGAWTFMPRKSEELEQVRQLVHNAVGLDTGRGDALEVSSAPFTDSEPPRD
EEEECEEEEECCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCC
PNFADMLADYAERLGKPLLNALLAFLFLMLIVRPVVLALIRPKVEAGEMVEGLEGLPAAE
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHH
EQLALYEALEEAARTEDEVPQEDDEDDLVFKDIEALKAHIFTLSDNHMEQVVTLVRGWMK
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHC
NDEAVKA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 1551848 [H]