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The map label for this gene is hydA [H]

Identifier: 220904771

GI number: 220904771

Start: 1794197

End: 1795453

Strand: Reverse

Name: hydA [H]

Synonym: Ddes_1503

Alternate gene names: 220904771

Gene position: 1795453-1794197 (Counterclockwise)

Preceding gene: 220904772

Following gene: 220904770

Centisome position: 62.48

GC content: 58.0

Gene sequence:

>1257_bases
ATGCCGCGCATTATTATGGAACATATCCAGTATGAGCCACATGTGCCGCCCAGAGGCGCTGACGGCGACAAGATGCTTTT
TGTCCAGATTGACGACAAAAAATGCATAGGTTGCGATCTTTGTCAGGAATATTGCCCCACCGGGGCCGTGTATGGAGAAA
CCGGCCTTGCCCACACGGTAGCCTACCCCGAACCGTGCATTAACTGCGGCCAGTGCCTGACCCACTGCCCGGAAATGGCT
ATCTATGAAACGCAGACCTGGGTTCCCGAGCTTCAGGAGAAGTTGAAGGACAAAAACGTCAAGTGCATCGCCATGCCTGC
TCCGGCGGTGCGCTATGCCCTTGGCGAGGCCTTTGGCCTTCCTCCGGGCACGGTCACCACGGGCAAGATGCTGGCGGCCC
TTAAAGCCCTGGGCTTTGACCATTGCTGGGATACGGAATTCGCCGCTGACGTGACCATATGGGAAGAGGCGTCGGAGTTT
GTACAGCGGCTGGGCGAAAAAAAGCATCTTCCCCAGTTTACGTCCTGCTGTCCCGGTTGGCAGAAGTATGCTGAAACCTT
TTATCCTGATCTTCTGCCGTATTTTTCTTCCTGCAAGTCGCCCGTCGGCATGAACGGCCGTCTGTCAAAAACCTATGGGG
CGGAAAAGCAGGGCTATGACCCGGCTCAGGTGTACACTGTGTCCATCATGCCGTGCATTGCCAAAAAATATGAAGGCCTG
CGCCCGGAAATGGCCGCAGGCGGCCAGCGCGACATAGACGCCACCCTCACAACCCGCGAACTGGCGTACCTTATCCGCCA
GGCCGGGATAGACTTTGCCAGACTGCCTGAAGGCCAGCGCGACAGCCTCATGGGCGAGTCCACAGGCGGGGCGACCATTT
TTGGCGTTTCCGGCGGTGTTATGGAAGCGGCGCTGCGCTACGCGTATGAAGCGGTGACCGGCCAGCGTCCCGATTCGTGG
GACTTTAAAGAGGTGCGCGGTCTCAAGGGCGTAAAGGAGTATGCCGTTACCATCAACGGTACGGAACTGCGCCTTGCCGT
GGTGCATGGGGCCAAGCATTTTGCCGCCGTGTGCGATCAGGTACGCGCCGGAAACTCGCCTTATCATTTCATCGAATTCA
TGGCCTGCCCAGGAGGTTGCGTCTGCGGCGGCGGCCAGCCCATCATGCCAAACCTGCTTGAGTACGCTGAACGCAAGGCC
ACAAGCCTGTTCGCGGGCCTCAGAAAACGGCTCGCCAACGTTTCAACCAACGCATAG

Upstream 100 bases:

>100_bases
AAGGGTCAAGTTCTGTGCCGGGTTCCGGCTGCACTGTAGGGAGCGTGCGCGCAGAAGCGCGCCGGAAAACTGTTTCACCT
GTTACCGCCAGGAGGTTGGA

Downstream 100 bases:

>100_bases
GAGGCGGTATGTCGATCATCAGCACAACGAGACGGGGATTTCTGAAAGGCGCATGTATCCTGTCCGGCGGCCTGCTGCTT
GGCGTGCGCATGGTGAACAA

Product: hydrogenase, Fe-only

Products: NA

Alternate protein names: Fe hydrogenlyase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPRIIMEHIQYEPHVPPRGADGDKMLFVQIDDKKCIGCDLCQEYCPTGAVYGETGLAHTVAYPEPCINCGQCLTHCPEMA
IYETQTWVPELQEKLKDKNVKCIAMPAPAVRYALGEAFGLPPGTVTTGKMLAALKALGFDHCWDTEFAADVTIWEEASEF
VQRLGEKKHLPQFTSCCPGWQKYAETFYPDLLPYFSSCKSPVGMNGRLSKTYGAEKQGYDPAQVYTVSIMPCIAKKYEGL
RPEMAAGGQRDIDATLTTRELAYLIRQAGIDFARLPEGQRDSLMGESTGGATIFGVSGGVMEAALRYAYEAVTGQRPDSW
DFKEVRGLKGVKEYAVTINGTELRLAVVHGAKHFAAVCDQVRAGNSPYHFIEFMACPGGCVCGGGQPIMPNLLEYAERKA
TSLFAGLRKRLANVSTNA

Sequences:

>Translated_418_residues
MPRIIMEHIQYEPHVPPRGADGDKMLFVQIDDKKCIGCDLCQEYCPTGAVYGETGLAHTVAYPEPCINCGQCLTHCPEMA
IYETQTWVPELQEKLKDKNVKCIAMPAPAVRYALGEAFGLPPGTVTTGKMLAALKALGFDHCWDTEFAADVTIWEEASEF
VQRLGEKKHLPQFTSCCPGWQKYAETFYPDLLPYFSSCKSPVGMNGRLSKTYGAEKQGYDPAQVYTVSIMPCIAKKYEGL
RPEMAAGGQRDIDATLTTRELAYLIRQAGIDFARLPEGQRDSLMGESTGGATIFGVSGGVMEAALRYAYEAVTGQRPDSW
DFKEVRGLKGVKEYAVTINGTELRLAVVHGAKHFAAVCDQVRAGNSPYHFIEFMACPGGCVCGGGQPIMPNLLEYAERKA
TSLFAGLRKRLANVSTNA
>Mature_417_residues
PRIIMEHIQYEPHVPPRGADGDKMLFVQIDDKKCIGCDLCQEYCPTGAVYGETGLAHTVAYPEPCINCGQCLTHCPEMAI
YETQTWVPELQEKLKDKNVKCIAMPAPAVRYALGEAFGLPPGTVTTGKMLAALKALGFDHCWDTEFAADVTIWEEASEFV
QRLGEKKHLPQFTSCCPGWQKYAETFYPDLLPYFSSCKSPVGMNGRLSKTYGAEKQGYDPAQVYTVSIMPCIAKKYEGLR
PEMAAGGQRDIDATLTTRELAYLIRQAGIDFARLPEGQRDSLMGESTGGATIFGVSGGVMEAALRYAYEAVTGQRPDSWD
FKEVRGLKGVKEYAVTINGTELRLAVVHGAKHFAAVCDQVRAGNSPYHFIEFMACPGGCVCGGGQPIMPNLLEYAERKAT
SLFAGLRKRLANVSTNA

Specific function: May be involved in hydrogen uptake for the reduction of sulfate to hydrogen sulfide in an electron transport chain. Cytochrome c3 is likely to be the physiological electron carrier for the enzyme [H]

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI11968051, Length=356, Percent_Identity=31.7415730337079, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI6912524, Length=363, Percent_Identity=28.099173553719, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI84452151, Length=279, Percent_Identity=30.1075268817204, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI134284357, Length=279, Percent_Identity=30.1075268817204, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI14165461, Length=431, Percent_Identity=26.2180974477958, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71995015, Length=361, Percent_Identity=26.3157894736842, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324089, Length=376, Percent_Identity=23.4042553191489, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI116007464, Length=365, Percent_Identity=29.3150684931507, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI116007470, Length=365, Percent_Identity=29.3150684931507, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI116007466, Length=365, Percent_Identity=29.3150684931507, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI116007468, Length=365, Percent_Identity=29.3150684931507, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI116007462, Length=247, Percent_Identity=30.3643724696356, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR013352 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF00037 Fer4 [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 45793; Mature: 45662

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIIMEHIQYEPHVPPRGADGDKMLFVQIDDKKCIGCDLCQEYCPTGAVYGETGLAHTV
CCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEE
AYPEPCINCGQCLTHCPEMAIYETQTWVPELQEKLKDKNVKCIAMPAPAVRYALGEAFGL
CCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCC
PPGTVTTGKMLAALKALGFDHCWDTEFAADVTIWEEASEFVQRLGEKKHLPQFTSCCPGW
CCCCCCHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCH
QKYAETFYPDLLPYFSSCKSPVGMNGRLSKTYGAEKQGYDPAQVYTVSIMPCIAKKYEGL
HHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEHHHHHHHHHHCCC
RPEMAAGGQRDIDATLTTRELAYLIRQAGIDFARLPEGQRDSLMGESTGGATIFGVSGGV
CCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHCCCCCCCCCEEEEECCCHH
MEAALRYAYEAVTGQRPDSWDFKEVRGLKGVKEYAVTINGTELRLAVVHGAKHFAAVCDQ
HHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEECCCEEEEEEECCHHHHHHHHHH
VRAGNSPYHFIEFMACPGGCVCGGGQPIMPNLLEYAERKATSLFAGLRKRLANVSTNA
HHCCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRIIMEHIQYEPHVPPRGADGDKMLFVQIDDKKCIGCDLCQEYCPTGAVYGETGLAHTV
CCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEE
AYPEPCINCGQCLTHCPEMAIYETQTWVPELQEKLKDKNVKCIAMPAPAVRYALGEAFGL
CCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCC
PPGTVTTGKMLAALKALGFDHCWDTEFAADVTIWEEASEFVQRLGEKKHLPQFTSCCPGW
CCCCCCHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCH
QKYAETFYPDLLPYFSSCKSPVGMNGRLSKTYGAEKQGYDPAQVYTVSIMPCIAKKYEGL
HHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEHHHHHHHHHHCCC
RPEMAAGGQRDIDATLTTRELAYLIRQAGIDFARLPEGQRDSLMGESTGGATIFGVSGGV
CCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHCCCCCCCCCEEEEECCCHH
MEAALRYAYEAVTGQRPDSWDFKEVRGLKGVKEYAVTINGTELRLAVVHGAKHFAAVCDQ
HHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEECCCEEEEEEECCHHHHHHHHHH
VRAGNSPYHFIEFMACPGGCVCGGGQPIMPNLLEYAERKATSLFAGLRKRLANVSTNA
HHCCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3888621; 3322275; 11456963; 10368269; 11456758 [H]