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The map label for this gene is metG [H]

Identifier: 220904688

GI number: 220904688

Start: 1698025

End: 1700037

Strand: Reverse

Name: metG [H]

Synonym: Ddes_1420

Alternate gene names: 220904688

Gene position: 1700037-1698025 (Counterclockwise)

Preceding gene: 220904689

Following gene: 220904686

Centisome position: 59.16

GC content: 56.53

Gene sequence:

>2013_bases
GTGAATACCTTCTTTATCACTACGCCCATCTACTACGTTAATGCCAGACCGCACCTGGGGCATGCCTATACTACAGTGGT
TGCCGATGCCATGGCCCGGTACCACAAGCTGGCCGGGCAGGACACGCTTTTTCTGACGGGTACGGACGAGCACGGCGACA
AGATTGTGCAGGCAGCCGAAAAGGAAGGCAAATCGCCCAAGGAATTTGTGGACGGCATCAGCGCCCGCTTTCAGGCATTG
TGGCCCCGGCTTGATGTGGCCAACGACCGTTTTGTACGCACCACTGACCCCGCGCATGTGGCTTCTGTGCAGAGTTTCCT
GCAAAAGGTTTATGATGCCGGAGACATCTATTTTGGTGAGTTTGGCGGTCATTACTGCTACGGCTGTGAACGCTTCTACA
CAGAAAAAGAGCTGGAAAACGGCCTTTGCCCGCAGCACCTGAGCAAACCGGAATTCATAAGTGAAAAAAACTATTTTTTC
CGCATGTCCAAATACCTGCCATGGCTCAAGGAGCATATTGAGCAGAACCCCACGTTTATCCGGCCGGAACGCTATCGTAC
CGAAGTCCTGTCCATGCTGGAATCCGGCGCACTGGAAGACCTGTGTATTTCGCGGCCCAAGTCACGCCTGACCTGGGGCA
TTGAGCTTCCTTTTGACAAGGACTATGTGTGCTACGTCTGGTTTGATGCGCTTATCAACTATATCAGCGCGCTTGGCTGG
CCGGATGGCGCGGATTTTGAGCGCTACTGGCCTGGTGAGCATCTGGTTGCCAAGGACATTCTCAAGCCGCACGCCGTCTT
CTGGCCCACCATGCTCAAATCGGCAGGCCTGCCCCAATACAGGCATCTCAACGTACACGGCTATTGGCTGGTGCGCGACA
CCAAAATGTCAAAATCATTGGGCAACGTTGTAGAGCCGGAAGAAATGGCGCAGCGCTTCGGAGCGGACGCCTTCCGGTAT
TTTCTGCTGCGTGAAATGCATTTCGGGTCTGACGCCAGCTTTAGCGACGAAGCGCTTGTAGGCCGCATAAACGCCGATCT
TGCCAATGACCTGGGCAATCTTTTCAGCCGGGTTCTTTCTATGACCGCCAAGTATTTCGGCAGTCGCGTTCCGGCGCCCA
AGCAGTTTGACGAGGCGGACAAGGCTATTGAAGACCTGTGCGCCACGGCCATGCGCAATTTCAGCCAGCTTTTCGGCAAT
GTGCAATTTGCTCAGGGGCTTGAATCCCTGTGGGAACTGGTGCGCGCCCTCAATAAGTATGTGGACAGTCAGGCGCCGTG
GGCTTTGCACAAGCAGGGTAATATGGAACGCCTTGCCACGGTCATGTATGTGCTGCTGGCCGCCATGCGCAAAACAGCCC
TGTGTCTGTGGCCGGTCATGCCCGTGGCCTGCGGCAAGATGCTGGAGCAACTTGGTCAGCCTGTGCAGAAAACCCTGCCG
CCCGTACCTGTGCTTGAAGAAGAGATGGGGAGTCTTGATGGCCTTGTGCCGGGGACTGGCGTTGCGGAAGCCTCAAACCT
GTTCCCGCGCATTGAGGTAAAAAAAGATCCGGCCGTTGCTGAAAAGGCCAAGGCCGCAAAAGCGCAGGCCAAGGCAGAGA
AAAACGCGGCCAGGCAGCAGAAAGGCGACGGCGGCAAACCGGCGGAGCCATGTGCCGCCGGCGACGACGCGGCGGTTTCG
GGCAGGCCCAATATTGATTTTGACCATTTCAAAGCTCTGGACATGCGCGTGGGAACAGTGCTGGTGGCTGAAAAGCACCC
CAATGCCGATCGTATCCTGCGGCTGGAAATAGACTTTGGCGAAGGGCAGCCCCGCCAGATACTCTCAGGCCTTGCCGAAC
ACTACGCACCTGAAGCTATGGTGGGAAAGCGAGTTTGCGCCGTGCTCAACCTTGAGCCGCGCAAAATTCGCGGCCTCATG
TCGCACGGCATGGTGCTCACGGCAGGGGATGGAAATTCCCTGACCCTGCTTGCCCTTGACGGCAACGTGCCGGATGGCAG
CGAAATAGCGTAG

Upstream 100 bases:

>100_bases
GCATGGCGATGCGGCAAAGCCGCGCCGCAAGCGCCGCCGTAAAACACAGAAAAACGAAAATCAATAAGCTATACGCCATT
ACGAGCTTACGGAGCCTTGA

Downstream 100 bases:

>100_bases
TACATCAGGTCAGGCGGTTTTGCAGCAAAACCGCCTGACGGCTACGAGCAGACGCCTGGCACAATGGTCGGGCGCAGCTT
CAGCCCTTGCCTGGGACGTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MNTFFITTPIYYVNARPHLGHAYTTVVADAMARYHKLAGQDTLFLTGTDEHGDKIVQAAEKEGKSPKEFVDGISARFQAL
WPRLDVANDRFVRTTDPAHVASVQSFLQKVYDAGDIYFGEFGGHYCYGCERFYTEKELENGLCPQHLSKPEFISEKNYFF
RMSKYLPWLKEHIEQNPTFIRPERYRTEVLSMLESGALEDLCISRPKSRLTWGIELPFDKDYVCYVWFDALINYISALGW
PDGADFERYWPGEHLVAKDILKPHAVFWPTMLKSAGLPQYRHLNVHGYWLVRDTKMSKSLGNVVEPEEMAQRFGADAFRY
FLLREMHFGSDASFSDEALVGRINADLANDLGNLFSRVLSMTAKYFGSRVPAPKQFDEADKAIEDLCATAMRNFSQLFGN
VQFAQGLESLWELVRALNKYVDSQAPWALHKQGNMERLATVMYVLLAAMRKTALCLWPVMPVACGKMLEQLGQPVQKTLP
PVPVLEEEMGSLDGLVPGTGVAEASNLFPRIEVKKDPAVAEKAKAAKAQAKAEKNAARQQKGDGGKPAEPCAAGDDAAVS
GRPNIDFDHFKALDMRVGTVLVAEKHPNADRILRLEIDFGEGQPRQILSGLAEHYAPEAMVGKRVCAVLNLEPRKIRGLM
SHGMVLTAGDGNSLTLLALDGNVPDGSEIA

Sequences:

>Translated_670_residues
MNTFFITTPIYYVNARPHLGHAYTTVVADAMARYHKLAGQDTLFLTGTDEHGDKIVQAAEKEGKSPKEFVDGISARFQAL
WPRLDVANDRFVRTTDPAHVASVQSFLQKVYDAGDIYFGEFGGHYCYGCERFYTEKELENGLCPQHLSKPEFISEKNYFF
RMSKYLPWLKEHIEQNPTFIRPERYRTEVLSMLESGALEDLCISRPKSRLTWGIELPFDKDYVCYVWFDALINYISALGW
PDGADFERYWPGEHLVAKDILKPHAVFWPTMLKSAGLPQYRHLNVHGYWLVRDTKMSKSLGNVVEPEEMAQRFGADAFRY
FLLREMHFGSDASFSDEALVGRINADLANDLGNLFSRVLSMTAKYFGSRVPAPKQFDEADKAIEDLCATAMRNFSQLFGN
VQFAQGLESLWELVRALNKYVDSQAPWALHKQGNMERLATVMYVLLAAMRKTALCLWPVMPVACGKMLEQLGQPVQKTLP
PVPVLEEEMGSLDGLVPGTGVAEASNLFPRIEVKKDPAVAEKAKAAKAQAKAEKNAARQQKGDGGKPAEPCAAGDDAAVS
GRPNIDFDHFKALDMRVGTVLVAEKHPNADRILRLEIDFGEGQPRQILSGLAEHYAPEAMVGKRVCAVLNLEPRKIRGLM
SHGMVLTAGDGNSLTLLALDGNVPDGSEIA
>Mature_670_residues
MNTFFITTPIYYVNARPHLGHAYTTVVADAMARYHKLAGQDTLFLTGTDEHGDKIVQAAEKEGKSPKEFVDGISARFQAL
WPRLDVANDRFVRTTDPAHVASVQSFLQKVYDAGDIYFGEFGGHYCYGCERFYTEKELENGLCPQHLSKPEFISEKNYFF
RMSKYLPWLKEHIEQNPTFIRPERYRTEVLSMLESGALEDLCISRPKSRLTWGIELPFDKDYVCYVWFDALINYISALGW
PDGADFERYWPGEHLVAKDILKPHAVFWPTMLKSAGLPQYRHLNVHGYWLVRDTKMSKSLGNVVEPEEMAQRFGADAFRY
FLLREMHFGSDASFSDEALVGRINADLANDLGNLFSRVLSMTAKYFGSRVPAPKQFDEADKAIEDLCATAMRNFSQLFGN
VQFAQGLESLWELVRALNKYVDSQAPWALHKQGNMERLATVMYVLLAAMRKTALCLWPVMPVACGKMLEQLGQPVQKTLP
PVPVLEEEMGSLDGLVPGTGVAEASNLFPRIEVKKDPAVAEKAKAAKAQAKAEKNAARQQKGDGGKPAEPCAAGDDAAVS
GRPNIDFDHFKALDMRVGTVLVAEKHPNADRILRLEIDFGEGQPRQILSGLAEHYAPEAMVGKRVCAVLNLEPRKIRGLM
SHGMVLTAGDGNSLTLLALDGNVPDGSEIA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=549, Percent_Identity=35.5191256830601, Blast_Score=308, Evalue=2e-83,
Organism=Homo sapiens, GI14043022, Length=603, Percent_Identity=27.363184079602, Blast_Score=165, Evalue=2e-40,
Organism=Homo sapiens, GI215490011, Length=93, Percent_Identity=40.8602150537634, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI215490009, Length=93, Percent_Identity=40.8602150537634, Blast_Score=80, Evalue=9e-15,
Organism=Homo sapiens, GI45006986, Length=93, Percent_Identity=40.8602150537634, Blast_Score=80, Evalue=9e-15,
Organism=Homo sapiens, GI4507947, Length=77, Percent_Identity=42.8571428571429, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1788432, Length=713, Percent_Identity=26.2272089761571, Blast_Score=205, Evalue=8e-54,
Organism=Escherichia coli, GI1789455, Length=107, Percent_Identity=31.7757009345794, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI212645096, Length=490, Percent_Identity=35.9183673469388, Blast_Score=297, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI71992781, Length=384, Percent_Identity=32.8125, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17540794, Length=592, Percent_Identity=27.027027027027, Blast_Score=185, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6321610, Length=517, Percent_Identity=34.4294003868472, Blast_Score=280, Evalue=6e-76,
Organism=Saccharomyces cerevisiae, GI6321704, Length=558, Percent_Identity=25.089605734767, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321333, Length=87, Percent_Identity=37.9310344827586, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21358483, Length=495, Percent_Identity=34.5454545454545, Blast_Score=291, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24655311, Length=589, Percent_Identity=27.8438030560272, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21357223, Length=80, Percent_Identity=42.5, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24651924, Length=86, Percent_Identity=37.2093023255814, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 74814; Mature: 74814

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFFITTPIYYVNARPHLGHAYTTVVADAMARYHKLAGQDTLFLTGTDEHGDKIVQAAE
CCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
KEGKSPKEFVDGISARFQALWPRLDVANDRFVRTTDPAHVASVQSFLQKVYDAGDIYFGE
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEC
FGGHYCYGCERFYTEKELENGLCPQHLSKPEFISEKNYFFRMSKYLPWLKEHIEQNPTFI
CCCEEEECHHHHHHHHHHHCCCCHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEE
RPERYRTEVLSMLESGALEDLCISRPKSRLTWGIELPFDKDYVCYVWFDALINYISALGW
CCHHHHHHHHHHHHHCCHHHHHHCCCHHHCEEEEECCCCCCCEEEHHHHHHHHHHHHCCC
PDGADFERYWPGEHLVAKDILKPHAVFWPTMLKSAGLPQYRHLNVHGYWLVRDTKMSKSL
CCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCHHHHHH
GNVVEPEEMAQRFGADAFRYFLLREMHFGSDASFSDEALVGRINADLANDLGNLFSRVLS
CCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MTAKYFGSRVPAPKQFDEADKAIEDLCATAMRNFSQLFGNVQFAQGLESLWELVRALNKY
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VDSQAPWALHKQGNMERLATVMYVLLAAMRKTALCLWPVMPVACGKMLEQLGQPVQKTLP
HCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
PVPVLEEEMGSLDGLVPGTGVAEASNLFPRIEVKKDPAVAEKAKAAKAQAKAEKNAARQQ
CCCCHHHHHCCCCCCCCCCCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
KGDGGKPAEPCAAGDDAAVSGRPNIDFDHFKALDMRVGTVLVAEKHPNADRILRLEIDFG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECEEEEEECCCCCCCEEEEEEECC
EGQPRQILSGLAEHYAPEAMVGKRVCAVLNLEPRKIRGLMSHGMVLTAGDGNSLTLLALD
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCEEEEEEEC
GNVPDGSEIA
CCCCCCCCCC
>Mature Secondary Structure
MNTFFITTPIYYVNARPHLGHAYTTVVADAMARYHKLAGQDTLFLTGTDEHGDKIVQAAE
CCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
KEGKSPKEFVDGISARFQALWPRLDVANDRFVRTTDPAHVASVQSFLQKVYDAGDIYFGE
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEC
FGGHYCYGCERFYTEKELENGLCPQHLSKPEFISEKNYFFRMSKYLPWLKEHIEQNPTFI
CCCEEEECHHHHHHHHHHHCCCCHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCEE
RPERYRTEVLSMLESGALEDLCISRPKSRLTWGIELPFDKDYVCYVWFDALINYISALGW
CCHHHHHHHHHHHHHCCHHHHHHCCCHHHCEEEEECCCCCCCEEEHHHHHHHHHHHHCCC
PDGADFERYWPGEHLVAKDILKPHAVFWPTMLKSAGLPQYRHLNVHGYWLVRDTKMSKSL
CCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCHHHHHH
GNVVEPEEMAQRFGADAFRYFLLREMHFGSDASFSDEALVGRINADLANDLGNLFSRVLS
CCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MTAKYFGSRVPAPKQFDEADKAIEDLCATAMRNFSQLFGNVQFAQGLESLWELVRALNKY
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VDSQAPWALHKQGNMERLATVMYVLLAAMRKTALCLWPVMPVACGKMLEQLGQPVQKTLP
HCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
PVPVLEEEMGSLDGLVPGTGVAEASNLFPRIEVKKDPAVAEKAKAAKAQAKAEKNAARQQ
CCCCHHHHHCCCCCCCCCCCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
KGDGGKPAEPCAAGDDAAVSGRPNIDFDHFKALDMRVGTVLVAEKHPNADRILRLEIDFG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECEEEEEECCCCCCCEEEEEEECC
EGQPRQILSGLAEHYAPEAMVGKRVCAVLNLEPRKIRGLMSHGMVLTAGDGNSLTLLALD
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCEEEEEEEC
GNVPDGSEIA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1993699; 10673435 [H]