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The map label for this gene is adhE [H]

Identifier: 220904628

GI number: 220904628

Start: 1635659

End: 1637236

Strand: Direct

Name: adhE [H]

Synonym: Ddes_1360

Alternate gene names: 220904628

Gene position: 1635659-1637236 (Clockwise)

Preceding gene: 220904627

Following gene: 220904629

Centisome position: 56.92

GC content: 59.7

Gene sequence:

>1578_bases
ATGGTTGATAAGGATTTGCTTTCCATTCAAGAGGCCCGCGCCCTGGTTCGCGCAGCGCGCAAGGCCCAGCCCGAATTCGC
GCAGTTGAGCCAGGAACGCGTGAATGACGTGGTCAGGGCCGTGGCCGAAGCCACGGCAGCCCAGGCTGAAGTTCTGGCAA
ACATGGCCGTTGAAGAAACCGGCTTCGGCAGACCGCAGGACAAAAAAATCAAGAACCTGCTGGCCAGTGAAAAAGTTCAT
GCCTGCATCAAGGACATGAAAACCATTGGCGTGCTGTGTTCTGATCCGGTGACCAAGATCGTTGAAATTGCCGTGCCCGT
TGGCGTGATTGCCGGTATCGTGCCGTCCACCAACCCCACGTCCACTGTTATTTACAAGGCGCTTATCTCGCTCAAGGCGG
GTAACGCCATTGTGTTCACCCCGCATCCCAGTGCCCGCAGTTGCATCGCCAAAACCGTGGAAGTCATTCAGGGTGCCCTG
CGCGCCTGCCACGTGTCGCCCGATCTGGTGAGCAGCATCAGTCTGCCCTCCATTGAAGGCACCAACGAGTTGATGAAGAT
GGCTGACCTTATCCTGGCGACAGGCGGCCCGGGCATGGTCAAGGCAGCGTACAGCTCCGGCACCCCTGCTCTGGGCGTCG
GCGCGGGCAACGTGCCTGCCTACATTGAGCGCAGTGCCAATATTGAAGATGCCGTGACCAAGATCATGAGCAGCAAAACC
TTTGATAACGGCACAATCTGCGCCTCGGAGCAATCCATCGTTACCGAGTCATGCATTGCCGACAAGGTTCGTGCCGTTAT
GGAAGCTCAGGGCTGCTATTTTCTTGCCGGAGAAAAGCTTGAAAAAGTCAAGCGCGTCATGGAACGCGGCAATGGCAGCA
TGAACCCCGCCATTGTGGGCCGTGATGCCCTGAGCATCGCCCGCGTTGCCGGCATTGAAGTGCCGCAGGGCACGCGCCTG
CTGGTTTCCGACGAAAAAGGCGTGGGACCCAAGTATCCCTTCAGCAAAGAAAAACTCACCGCCCTGCTGGGCTTCTATGT
GGTGGAAGACTGGCGCGAAGCCTGCGAACTGTGCACGGCCCTCCTGCACAACGGCGGTGTGGGCCATTCGCTGTCCATCC
ACTCGCAGAACGAAGAGGTCATTCGTGAGTTCGGCCTTAAAAAGCCTGTGTCGCGCATGCTGGTGAATACGCCCTCCACA
CAGGGAGCCGTGGGTCTTTCCACCGGCCTGTTCCCCTCCTTCACCCTGGGCTGCGGCGCAGTGGGCGGCAGTGCCACTTC
CGACAACGTAACCCCCCTGAACCTGCTCAACGTGCGCCGCGTGGCCTACGATCTGCAGTCACAGGGCTGCTCCTGCGCTT
CTTCCGCATCGTGCGGCGTTTCCACCATGGCGGGCGTTTCTGCTCCTGCGGCTTCCGCCGGGCACTACCCGCCCTCAGCC
ATGCTTTCGGGCTGCTGCCACCACGGGGCCAACCACGATGCCGTGGTCGCTCCCCAGGCGGCCGCAGCAGCCGCTGGCGG
GCTGGATATCAATGCCGTTACGGAAATGATCGTGGCGGAACTGAAAAAAGTTTTGTAA

Upstream 100 bases:

>100_bases
CATGAAGGTAGACCTCTCCCGTGAGCAGATCGCCTCGTCCAACAGGCAAACCCTGATGAACGCCATCGACCGGGCGGCAC
GGAAGGAAAAGGAGTAGGAC

Downstream 100 bases:

>100_bases
ACCGCGGCCCGGCCCTGCCGTTGTCGCATCAGATCAAAAACTATTTGAACACTATTGTTAAAAATCAAAGGAGATACCGC
TATGACCACTTCTTCCAACG

Product: acetaldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MVDKDLLSIQEARALVRAARKAQPEFAQLSQERVNDVVRAVAEATAAQAEVLANMAVEETGFGRPQDKKIKNLLASEKVH
ACIKDMKTIGVLCSDPVTKIVEIAVPVGVIAGIVPSTNPTSTVIYKALISLKAGNAIVFTPHPSARSCIAKTVEVIQGAL
RACHVSPDLVSSISLPSIEGTNELMKMADLILATGGPGMVKAAYSSGTPALGVGAGNVPAYIERSANIEDAVTKIMSSKT
FDNGTICASEQSIVTESCIADKVRAVMEAQGCYFLAGEKLEKVKRVMERGNGSMNPAIVGRDALSIARVAGIEVPQGTRL
LVSDEKGVGPKYPFSKEKLTALLGFYVVEDWREACELCTALLHNGGVGHSLSIHSQNEEVIREFGLKKPVSRMLVNTPST
QGAVGLSTGLFPSFTLGCGAVGGSATSDNVTPLNLLNVRRVAYDLQSQGCSCASSASCGVSTMAGVSAPAASAGHYPPSA
MLSGCCHHGANHDAVVAPQAAAAAAGGLDINAVTEMIVAELKKVL

Sequences:

>Translated_525_residues
MVDKDLLSIQEARALVRAARKAQPEFAQLSQERVNDVVRAVAEATAAQAEVLANMAVEETGFGRPQDKKIKNLLASEKVH
ACIKDMKTIGVLCSDPVTKIVEIAVPVGVIAGIVPSTNPTSTVIYKALISLKAGNAIVFTPHPSARSCIAKTVEVIQGAL
RACHVSPDLVSSISLPSIEGTNELMKMADLILATGGPGMVKAAYSSGTPALGVGAGNVPAYIERSANIEDAVTKIMSSKT
FDNGTICASEQSIVTESCIADKVRAVMEAQGCYFLAGEKLEKVKRVMERGNGSMNPAIVGRDALSIARVAGIEVPQGTRL
LVSDEKGVGPKYPFSKEKLTALLGFYVVEDWREACELCTALLHNGGVGHSLSIHSQNEEVIREFGLKKPVSRMLVNTPST
QGAVGLSTGLFPSFTLGCGAVGGSATSDNVTPLNLLNVRRVAYDLQSQGCSCASSASCGVSTMAGVSAPAASAGHYPPSA
MLSGCCHHGANHDAVVAPQAAAAAAGGLDINAVTEMIVAELKKVL
>Mature_525_residues
MVDKDLLSIQEARALVRAARKAQPEFAQLSQERVNDVVRAVAEATAAQAEVLANMAVEETGFGRPQDKKIKNLLASEKVH
ACIKDMKTIGVLCSDPVTKIVEIAVPVGVIAGIVPSTNPTSTVIYKALISLKAGNAIVFTPHPSARSCIAKTVEVIQGAL
RACHVSPDLVSSISLPSIEGTNELMKMADLILATGGPGMVKAAYSSGTPALGVGAGNVPAYIERSANIEDAVTKIMSSKT
FDNGTICASEQSIVTESCIADKVRAVMEAQGCYFLAGEKLEKVKRVMERGNGSMNPAIVGRDALSIARVAGIEVPQGTRL
LVSDEKGVGPKYPFSKEKLTALLGFYVVEDWREACELCTALLHNGGVGHSLSIHSQNEEVIREFGLKKPVSRMLVNTPST
QGAVGLSTGLFPSFTLGCGAVGGSATSDNVTPLNLLNVRRVAYDLQSQGCSCASSASCGVSTMAGVSAPAASAGHYPPSA
MLSGCCHHGANHDAVVAPQAAAAAAGGLDINAVTEMIVAELKKVL

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=442, Percent_Identity=45.7013574660633, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1788797, Length=376, Percent_Identity=32.1808510638298, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1786438, Length=273, Percent_Identity=26.7399267399267, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 54549; Mature: 54549

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKDLLSIQEARALVRAARKAQPEFAQLSQERVNDVVRAVAEATAAQAEVLANMAVEET
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFGRPQDKKIKNLLASEKVHACIKDMKTIGVLCSDPVTKIVEIAVPVGVIAGIVPSTNPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCH
STVIYKALISLKAGNAIVFTPHPSARSCIAKTVEVIQGALRACHVSPDLVSSISLPSIEG
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
TNELMKMADLILATGGPGMVKAAYSSGTPALGVGAGNVPAYIERSANIEDAVTKIMSSKT
HHHHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCCHHHCCCCCHHHHHHHHHHHCC
FDNGTICASEQSIVTESCIADKVRAVMEAQGCYFLAGEKLEKVKRVMERGNGSMNPAIVG
CCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCEEEC
RDALSIARVAGIEVPQGTRLLVSDEKGVGPKYPFSKEKLTALLGFYVVEDWREACELCTA
CHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLHNGGVGHSLSIHSQNEEVIREFGLKKPVSRMLVNTPSTQGAVGLSTGLFPSFTLGCGA
HHHCCCCCCEEEECCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCC
VGGSATSDNVTPLNLLNVRRVAYDLQSQGCSCASSASCGVSTMAGVSAPAASAGHYPPSA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHH
MLSGCCHHGANHDAVVAPQAAAAAAGGLDINAVTEMIVAELKKVL
HHHHHHHCCCCCCCEECCHHHHHHCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MVDKDLLSIQEARALVRAARKAQPEFAQLSQERVNDVVRAVAEATAAQAEVLANMAVEET
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFGRPQDKKIKNLLASEKVHACIKDMKTIGVLCSDPVTKIVEIAVPVGVIAGIVPSTNPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCH
STVIYKALISLKAGNAIVFTPHPSARSCIAKTVEVIQGALRACHVSPDLVSSISLPSIEG
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
TNELMKMADLILATGGPGMVKAAYSSGTPALGVGAGNVPAYIERSANIEDAVTKIMSSKT
HHHHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCCHHHCCCCCHHHHHHHHHHHCC
FDNGTICASEQSIVTESCIADKVRAVMEAQGCYFLAGEKLEKVKRVMERGNGSMNPAIVG
CCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCEEEC
RDALSIARVAGIEVPQGTRLLVSDEKGVGPKYPFSKEKLTALLGFYVVEDWREACELCTA
CHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLHNGGVGHSLSIHSQNEEVIREFGLKKPVSRMLVNTPSTQGAVGLSTGLFPSFTLGCGA
HHHCCCCCCEEEECCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCC
VGGSATSDNVTPLNLLNVRRVAYDLQSQGCSCASSASCGVSTMAGVSAPAASAGHYPPSA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHH
MLSGCCHHGANHDAVVAPQAAAAAAGGLDINAVTEMIVAELKKVL
HHHHHHHCCCCCCCEECCHHHHHHCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]