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The map label for this gene is brnQ [H]

Identifier: 220904561

GI number: 220904561

Start: 1522279

End: 1523586

Strand: Direct

Name: brnQ [H]

Synonym: Ddes_1292

Alternate gene names: 220904561

Gene position: 1522279-1523586 (Clockwise)

Preceding gene: 220904560

Following gene: 220904566

Centisome position: 52.98

GC content: 53.06

Gene sequence:

>1308_bases
ATGAGATCTTCTTTGGTACGCGATTCTTTTATTGTTGGTGCAGCGTTATTTTCCATGCTGTTTGGCGCAGGAAACGTCGT
TTTTCCACCCTACATCGGCCTTACTGCAGGTCCGGAATGGTTTTTAGGCTTTATCTGTTACTATATGGCTGATGTCGGCC
TGGCCTTGCTTGCCATCTACGCCATGCTTGCCTCTTCGTGCATTGATAATGAAGAAGGCATCATGCACAGGCTGGGGCGT
ACAGCAGCCATGCTTATGATGTGCGCCATAATTCTCTGCATAGGCCCCCTGCTTGCCATTCCCCGCACGGGAGCCACGGC
ATTTGCCATCTCCTTCGCGCCGCTGGGTTATTCCTCCAAGGGCGTCAGCGCGGCCAAGATACTTTATTCCATAGTTTTCT
TCGGCATATCCACCCTGTTGGCCTTTCGGGAGTCCAACATGGTAGATGTAATTGCCCGCTACCTTTCGCCCATAAAAATC
GGGGGCTTCCTGCTTATCGTGGCCGCGGCTTTTGTTATGCCCATCGGGCCGGTAAGCGACGTTGTTCGCGATGATAACGT
GGCGTGGCACAGCATACTTTCAGGTTATCAGACGCTGGACGTTATGGCGGCTCTGGTTTTTGGACTTATTATCGTAAACG
CCCTGAAGGCCAAAGGGTACGAAAGCGGCAGACAAAAGGCGCTGTCTGTGGGCATAGCAAGCCTCGTGGCAGGCGCTCTG
CTCTTCGTCATCTATTGCGGGCTTTGCTATCTTGGGGCAACAGGATCCACCTACTATCCAGTAGAAACGGAAAAAGGCGC
CCTGGTCACCCACCTGGTGGGCCGGCTTTTCGGCAGCGCAAGCACCATATTGCTGGCAATTATTGTAACAGTCGCCTGCA
TGGCCACCTCTGTGGCCCTCATCGGAACCACGGGAACCTTCATCTCACGCTTCAGCAAGGGGCGGCTCAGCTATAAAAGC
ATCGTGGTGGGAACCGGCCTTTTCAGCATGGTTGTTTCCAACTTCGGCCTCGACAACATCATTGCTTTTGCCGCGCCCAT
TCTGACTTTTTTATACCCGGGAACACTTGCAGTCATTATTCTTTCCCTTTTTGACCGTCATATAGACAATGACAATATAT
TCCGTTTTGCCACGGCCGGAGCGCTACTGGCCAGCGGGGTGACCGTGCTGGAAGAATGGGGTTTGCCCCTGGATATCACA
CGGCACCTTCCCTTTGACTCCTCAGGGCTGAGCTGGGTAGTGCCGGCCTGCGTTTTTGGCATCATCGGCTACTTTGTCCG
CCGTCCCAAAAAATCTTCCACCCATTAA

Upstream 100 bases:

>100_bases
GCCACCGAAACTGGCTGCGGCCAGTCCGGATTCATTTATGCTATTTCTGTTGCACAGCGGCGATATTTTTCAATAACGCA
CGATCCTGCGAGCATTTTTG

Downstream 100 bases:

>100_bases
AAAATTTTACCGTGAGCCGTGTGCACAGGCTGTCGGTCTGCGTCCGAAAATCCTCCTGCACATATATAATTCTGGCGCAC
AGGTTTTCGTCCCATACAGG

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MRSSLVRDSFIVGAALFSMLFGAGNVVFPPYIGLTAGPEWFLGFICYYMADVGLALLAIYAMLASSCIDNEEGIMHRLGR
TAAMLMMCAIILCIGPLLAIPRTGATAFAISFAPLGYSSKGVSAAKILYSIVFFGISTLLAFRESNMVDVIARYLSPIKI
GGFLLIVAAAFVMPIGPVSDVVRDDNVAWHSILSGYQTLDVMAALVFGLIIVNALKAKGYESGRQKALSVGIASLVAGAL
LFVIYCGLCYLGATGSTYYPVETEKGALVTHLVGRLFGSASTILLAIIVTVACMATSVALIGTTGTFISRFSKGRLSYKS
IVVGTGLFSMVVSNFGLDNIIAFAAPILTFLYPGTLAVIILSLFDRHIDNDNIFRFATAGALLASGVTVLEEWGLPLDIT
RHLPFDSSGLSWVVPACVFGIIGYFVRRPKKSSTH

Sequences:

>Translated_435_residues
MRSSLVRDSFIVGAALFSMLFGAGNVVFPPYIGLTAGPEWFLGFICYYMADVGLALLAIYAMLASSCIDNEEGIMHRLGR
TAAMLMMCAIILCIGPLLAIPRTGATAFAISFAPLGYSSKGVSAAKILYSIVFFGISTLLAFRESNMVDVIARYLSPIKI
GGFLLIVAAAFVMPIGPVSDVVRDDNVAWHSILSGYQTLDVMAALVFGLIIVNALKAKGYESGRQKALSVGIASLVAGAL
LFVIYCGLCYLGATGSTYYPVETEKGALVTHLVGRLFGSASTILLAIIVTVACMATSVALIGTTGTFISRFSKGRLSYKS
IVVGTGLFSMVVSNFGLDNIIAFAAPILTFLYPGTLAVIILSLFDRHIDNDNIFRFATAGALLASGVTVLEEWGLPLDIT
RHLPFDSSGLSWVVPACVFGIIGYFVRRPKKSSTH
>Mature_435_residues
MRSSLVRDSFIVGAALFSMLFGAGNVVFPPYIGLTAGPEWFLGFICYYMADVGLALLAIYAMLASSCIDNEEGIMHRLGR
TAAMLMMCAIILCIGPLLAIPRTGATAFAISFAPLGYSSKGVSAAKILYSIVFFGISTLLAFRESNMVDVIARYLSPIKI
GGFLLIVAAAFVMPIGPVSDVVRDDNVAWHSILSGYQTLDVMAALVFGLIIVNALKAKGYESGRQKALSVGIASLVAGAL
LFVIYCGLCYLGATGSTYYPVETEKGALVTHLVGRLFGSASTILLAIIVTVACMATSVALIGTTGTFISRFSKGRLSYKS
IVVGTGLFSMVVSNFGLDNIIAFAAPILTFLYPGTLAVIILSLFDRHIDNDNIFRFATAGALLASGVTVLEEWGLPLDIT
RHLPFDSSGLSWVVPACVFGIIGYFVRRPKKSSTH

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=446, Percent_Identity=29.8206278026906, Blast_Score=187, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46241; Mature: 46241

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSLVRDSFIVGAALFSMLFGAGNVVFPPYIGLTAGPEWFLGFICYYMADVGLALLAIY
CCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AMLASSCIDNEEGIMHRLGRTAAMLMMCAIILCIGPLLAIPRTGATAFAISFAPLGYSSK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
GVSAAKILYSIVFFGISTLLAFRESNMVDVIARYLSPIKIGGFLLIVAAAFVMPIGPVSD
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHH
VVRDDNVAWHSILSGYQTLDVMAALVFGLIIVNALKAKGYESGRQKALSVGIASLVAGAL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LFVIYCGLCYLGATGSTYYPVETEKGALVTHLVGRLFGSASTILLAIIVTVACMATSVAL
HHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IGTTGTFISRFSKGRLSYKSIVVGTGLFSMVVSNFGLDNIIAFAAPILTFLYPGTLAVII
HCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LSLFDRHIDNDNIFRFATAGALLASGVTVLEEWGLPLDITRHLPFDSSGLSWVVPACVFG
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHH
IIGYFVRRPKKSSTH
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MRSSLVRDSFIVGAALFSMLFGAGNVVFPPYIGLTAGPEWFLGFICYYMADVGLALLAIY
CCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AMLASSCIDNEEGIMHRLGRTAAMLMMCAIILCIGPLLAIPRTGATAFAISFAPLGYSSK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
GVSAAKILYSIVFFGISTLLAFRESNMVDVIARYLSPIKIGGFLLIVAAAFVMPIGPVSD
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHH
VVRDDNVAWHSILSGYQTLDVMAALVFGLIIVNALKAKGYESGRQKALSVGIASLVAGAL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LFVIYCGLCYLGATGSTYYPVETEKGALVTHLVGRLFGSASTILLAIIVTVACMATSVAL
HHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IGTTGTFISRFSKGRLSYKSIVVGTGLFSMVVSNFGLDNIIAFAAPILTFLYPGTLAVII
HCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LSLFDRHIDNDNIFRFATAGALLASGVTVLEEWGLPLDITRHLPFDSSGLSWVVPACVFG
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHH
IIGYFVRRPKKSSTH
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]