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The map label for this gene is gatB

Identifier: 220904551

GI number: 220904551

Start: 1511104

End: 1512540

Strand: Reverse

Name: gatB

Synonym: Ddes_1282

Alternate gene names: 220904551

Gene position: 1512540-1511104 (Counterclockwise)

Preceding gene: 220904552

Following gene: 220904547

Centisome position: 52.64

GC content: 55.32

Gene sequence:

>1437_bases
ATGGCCGTTTATGAAGCCGTTATCGGCCTGGAAGTGCATGTGCAACTGGCCACGGCCTCCAAACTGTTCTGTTCCTGCCC
GACAACCTTCGGCCAGCCTCCGAACTCCAATGTGTGCGAAGTATGCTCCGGCATGCCCGGATCGTTGCCCGTGCCTAACG
GACAGGCCATACATTTCGCCACGCTGGTGGGCCTTGCTACAGGCTGCACCATCAATCCGCGCTCCATTTTCGCGCGTAAA
AACTATTTTTATCCGGACCTGCCCGCAGGGTATCAGATATCGCAGTTTGAACTGCCCATCTGCGAGCACGGGCACCTGGA
CATTGAAGTGGATGGCGTCTGCAAAAGAGTGGGCATTACCCGCATTCATATGGAAAACGACGCGGGCAAAAACATACACG
CTCAGGGAGAGAACATCAGCTACGTGGACCTGAACAGGGCCGGTACGCCGCTGGTGGAAATAGTCTCCGACCCTGATATG
CGCTCTGCGGCTGAAGCTGTCGCCTACCTCAAGGACCTGTATGCCATTGTTACCTATCTTGGCGTGTGCGACGGCAACAT
GGAAGAAGGCAGCTTTCGCTGTGATGCCAACGTCTCTTTGCGGCCCGTGGGGGCGAAGGAATTCGGCACCCGTACGGAAC
TGAAAAATCTTAATTCGTTCCGTAACGTGCAGCGCGCTATTGAATGCGAAATGGCGCGCCAGCAAGACCTTCTGGATGAC
GGCGAAAAAGTCGTACAGGAAACACGCCTTTACGATGCGGTAAAAAATATTACTGCCTCCATGCGCAGCAAGGAAGAGGC
ACATGACTACCGTTACTTCCCTGACCCGGACATCCTGCCCATAGAGATTTCTGATTCGGACATGGAACGCTGGAAAGCAG
AACTGCCCGAACTGCCCAAGGCCAGGGCCGCGCGGTTTGTGGAGATGACAGGCTTGCCCGCTGCGGAAGTTGAAGTGCTG
GTGCAAAGCCGTGGTCTGGCGGATTTTTTTGAATCCGCCGCCGGTCTTTCTGATGCCCGCAAAGCGGCAAACTTCATGCT
TGGCCCATTGCTGCGCGAATGTAACAGCCGGGGCGTCAACGCGGCGGATCCGGCCGCATGGAACATGAAGCCCGAAGCGC
TGGCAGAACTGGTGCGCATTGTGGACCAGGGGCTGATCAGCTCCAAAATTGCCAATGATATTTTTGGCGACCTGTTTGAA
ACAGGCGTCATGCCCGAAAGGTATGTGAAAGAAAAAGGGCTTGTGCAGATATCCGACTCTTCGGCGTTGGAAGAAGCCGT
GGACGCGGTTATTGCGGCCAATCCCGCCGAGGTGGAAGCATACAGAGGCGGCAAAACCAAACTCATCAGTTTTTTTGTGG
GTCAGATCATGCGCGCCACCAAGGGCAAGGCCAATCCGGCCCTGGTGAACGAGTTACTGGCAAAAAAACTGTCGTAG

Upstream 100 bases:

>100_bases
CCAGACTTTTCTTCCGCCGGGGAGAATACGGGGTGGGCAAGTATTTTGACGCGAGCATTCCTCATCAGAGCTGATTACTT
TACAGGGAGTGAGTTGACTT

Downstream 100 bases:

>100_bases
GCAGGCGTTCGTCTGCGTTTATGCTGGGGCTTTTTCATGAAAGAGGCTCCCTGCCACAGCGGGGAGCCTCTTTGTCATGT
TTTCAGGTAAGCAGTGTCAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAVYEAVIGLEVHVQLATASKLFCSCPTTFGQPPNSNVCEVCSGMPGSLPVPNGQAIHFATLVGLATGCTINPRSIFARK
NYFYPDLPAGYQISQFELPICEHGHLDIEVDGVCKRVGITRIHMENDAGKNIHAQGENISYVDLNRAGTPLVEIVSDPDM
RSAAEAVAYLKDLYAIVTYLGVCDGNMEEGSFRCDANVSLRPVGAKEFGTRTELKNLNSFRNVQRAIECEMARQQDLLDD
GEKVVQETRLYDAVKNITASMRSKEEAHDYRYFPDPDILPIEISDSDMERWKAELPELPKARAARFVEMTGLPAAEVEVL
VQSRGLADFFESAAGLSDARKAANFMLGPLLRECNSRGVNAADPAAWNMKPEALAELVRIVDQGLISSKIANDIFGDLFE
TGVMPERYVKEKGLVQISDSSALEEAVDAVIAANPAEVEAYRGGKTKLISFFVGQIMRATKGKANPALVNELLAKKLS

Sequences:

>Translated_478_residues
MAVYEAVIGLEVHVQLATASKLFCSCPTTFGQPPNSNVCEVCSGMPGSLPVPNGQAIHFATLVGLATGCTINPRSIFARK
NYFYPDLPAGYQISQFELPICEHGHLDIEVDGVCKRVGITRIHMENDAGKNIHAQGENISYVDLNRAGTPLVEIVSDPDM
RSAAEAVAYLKDLYAIVTYLGVCDGNMEEGSFRCDANVSLRPVGAKEFGTRTELKNLNSFRNVQRAIECEMARQQDLLDD
GEKVVQETRLYDAVKNITASMRSKEEAHDYRYFPDPDILPIEISDSDMERWKAELPELPKARAARFVEMTGLPAAEVEVL
VQSRGLADFFESAAGLSDARKAANFMLGPLLRECNSRGVNAADPAAWNMKPEALAELVRIVDQGLISSKIANDIFGDLFE
TGVMPERYVKEKGLVQISDSSALEEAVDAVIAANPAEVEAYRGGKTKLISFFVGQIMRATKGKANPALVNELLAKKLS
>Mature_477_residues
AVYEAVIGLEVHVQLATASKLFCSCPTTFGQPPNSNVCEVCSGMPGSLPVPNGQAIHFATLVGLATGCTINPRSIFARKN
YFYPDLPAGYQISQFELPICEHGHLDIEVDGVCKRVGITRIHMENDAGKNIHAQGENISYVDLNRAGTPLVEIVSDPDMR
SAAEAVAYLKDLYAIVTYLGVCDGNMEEGSFRCDANVSLRPVGAKEFGTRTELKNLNSFRNVQRAIECEMARQQDLLDDG
EKVVQETRLYDAVKNITASMRSKEEAHDYRYFPDPDILPIEISDSDMERWKAELPELPKARAARFVEMTGLPAAEVEVLV
QSRGLADFFESAAGLSDARKAANFMLGPLLRECNSRGVNAADPAAWNMKPEALAELVRIVDQGLISSKIANDIFGDLFET
GVMPERYVKEKGLVQISDSSALEEAVDAVIAANPAEVEAYRGGKTKLISFFVGQIMRATKGKANPALVNELLAKKLS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=501, Percent_Identity=35.7285429141717, Blast_Score=297, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI115534094, Length=307, Percent_Identity=40.7166123778502, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319391, Length=508, Percent_Identity=26.7716535433071, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI45550808, Length=496, Percent_Identity=35.4838709677419, Blast_Score=272, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_DESDA (B8J0A8)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479863.1
- GeneID:   7284974
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1282
- HOGENOM:   HBG395951
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52186; Mature: 52055

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVYEAVIGLEVHVQLATASKLFCSCPTTFGQPPNSNVCEVCSGMPGSLPVPNGQAIHFA
CCHHHHHCCEEEEEEEEHHHHHHEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEHHH
TLVGLATGCTINPRSIFARKNYFYPDLPAGYQISQFELPICEHGHLDIEVDGVCKRVGIT
HHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEECEECCCCCCCCCEEEEHHHHHHHHCEE
RIHMENDAGKNIHAQGENISYVDLNRAGTPLVEIVSDPDMRSAAEAVAYLKDLYAIVTYL
EEEECCCCCCCEEECCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
GVCDGNMEEGSFRCDANVSLRPVGAKEFGTRTELKNLNSFRNVQRAIECEMARQQDLLDD
HHCCCCCCCCCEEECCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEKVVQETRLYDAVKNITASMRSKEEAHDYRYFPDPDILPIEISDSDMERWKAELPELPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCH
ARAARFVEMTGLPAAEVEVLVQSRGLADFFESAAGLSDARKAANFMLGPLLRECNSRGVN
HHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
AADPAAWNMKPEALAELVRIVDQGLISSKIANDIFGDLFETGVMPERYVKEKGLVQISDS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEECCH
SALEEAVDAVIAANPAEVEAYRGGKTKLISFFVGQIMRATKGKANPALVNELLAKKLS
HHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AVYEAVIGLEVHVQLATASKLFCSCPTTFGQPPNSNVCEVCSGMPGSLPVPNGQAIHFA
CHHHHHCCEEEEEEEEHHHHHHEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEHHH
TLVGLATGCTINPRSIFARKNYFYPDLPAGYQISQFELPICEHGHLDIEVDGVCKRVGIT
HHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEECEECCCCCCCCCEEEEHHHHHHHHCEE
RIHMENDAGKNIHAQGENISYVDLNRAGTPLVEIVSDPDMRSAAEAVAYLKDLYAIVTYL
EEEECCCCCCCEEECCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
GVCDGNMEEGSFRCDANVSLRPVGAKEFGTRTELKNLNSFRNVQRAIECEMARQQDLLDD
HHCCCCCCCCCEEECCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEKVVQETRLYDAVKNITASMRSKEEAHDYRYFPDPDILPIEISDSDMERWKAELPELPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCH
ARAARFVEMTGLPAAEVEVLVQSRGLADFFESAAGLSDARKAANFMLGPLLRECNSRGVN
HHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
AADPAAWNMKPEALAELVRIVDQGLISSKIANDIFGDLFETGVMPERYVKEKGLVQISDS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEECCH
SALEEAVDAVIAANPAEVEAYRGGKTKLISFFVGQIMRATKGKANPALVNELLAKKLS
HHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA