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The map label for this gene is miaB [H]

Identifier: 220904542

GI number: 220904542

Start: 1500528

End: 1501892

Strand: Direct

Name: miaB [H]

Synonym: Ddes_1273

Alternate gene names: 220904542

Gene position: 1500528-1501892 (Clockwise)

Preceding gene: 220904541

Following gene: 220904543

Centisome position: 52.22

GC content: 60.37

Gene sequence:

>1365_bases
ATGATCGAAAAATCTTATCATATCATTACGTTCGGCTGCCAGATGAACGTGCACGACTCCCAGTGGCTGGGCCGTGCCCT
CACAGCTCGCGGGTTTGTTGAAGCTTCACTTGAAGATGCGCAGGTGGTGGTTGTCAATACCTGTTCCGTGCGTGAAAAAC
CGGAACAAAAGGTCATGAGCGCCCTTGGCCGCATCCGCCACGTTACTGGCGGCAATCCCGATGTGCTGGTAGGGGTCGCA
GGCTGCGTGGCGCAGCAATTGGGTGAAGGGTTTTTCAGCAAGGAAAGCCAGGTGCGCCTGGTGGCGGGCAGCGACGGCAT
AAGCGGTGCGCCTGCAGCAATCGAACGCCTGCTGGAAGAGCCGACCCTGCACTTGTCATTGCTGGATTTCACCAGCCACT
ATATCGAACGCGAGGCCGGTCCGGGAACATCCGGATCCTCCTCAGGCCCGGTGGCCTTTGTCAACATCATGCAAGGCTGC
GACAACTTTTGCGCGTATTGCATTGTTCCCTTTACCCGGGGACGCCAGAAATCGCGCTCGGCAGCCGCTATCCTTGATGA
ATGCCGCGCCGTGCTCGAGCGGGGCGCGCGCGAGATCACACTGCTGGGGCAGAATGTCAATGCCTTCGGCCAGGATAAAA
GCGGCGATGGAACAAGCTTTGCACAGCTTTTACGCCAGGTGGCCGGTCTGCCCGGGCTTGAGCGCCTGCGCTATGTCACC
CCTCACCCCAAGGATATGGGGCCGGAAGACGTAGCCGCTTTTGCGGAATTGCCCCAGCTATGCCCCCGTCTGCACCTTCC
CCTGCAGGCCGGATCAGATGTGGTACTCAGGAGCATGCGCCGCCGGTACGACAGCGCGGCCTTCTTGCGCCTTGTGGACG
ATCTGCGCGCCGCAAGGCCGGACCTCGCCCTGTCCACTGACCTGATTGTGGCTTTTCCCGGTGAAACGGAAGAAGACTTT
CAGGACACACTTGCCATGATGCGTGCCTGCAACTTCATGTCCAGCTTTTCTTTCATATATTCCGACAGGCCTGGAGCCAG
AGCCGCGCTTTTTCCAGGTAAAATCGCTCCGGACGTGGCCCAGGACAGGCTGCGGCGGCTTCAGGCCCTCCAGGACGACC
TGGGAGCACGCTGGCTGGCGGCCCGGGTTGAAAACAGGGACAAGACAACCCTGCTCATGGAAGGCCCAAGCCCCAAACCG
GGCGAAAGTGCCGAACCAAGCTGGCAGGGGCGTGACCCGTACGGAGTACCGGTGCATGTACCGTTGCCCGCCGGGGCTGA
TCACACAGGTTTTCTGGTGCAAACCGTCATTACAGAGGCCAAGAAGCACAGCCTGCTGGCCGTGCGCACGGGGGAGCCAT
GGTAG

Upstream 100 bases:

>100_bases
CCCGCGCCGTGCTGGACTGGCTGGAAGACCGCGTGGCCGCCCTGGAAGAAGAGGCCTCCGGCATGTACGAGGCAGACCTG
GACATTGAACAATAGTAGTC

Downstream 100 bases:

>100_bases
AGATGCGTGTTTTCGGCCTGACCATTGACCCGCAGACCAAAACCCCCATAGTGATACTGCGCGAAATGGAAGGAGAGACC
GTACTGCCCGTCTGGGTGGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MIEKSYHIITFGCQMNVHDSQWLGRALTARGFVEASLEDAQVVVVNTCSVREKPEQKVMSALGRIRHVTGGNPDVLVGVA
GCVAQQLGEGFFSKESQVRLVAGSDGISGAPAAIERLLEEPTLHLSLLDFTSHYIEREAGPGTSGSSSGPVAFVNIMQGC
DNFCAYCIVPFTRGRQKSRSAAAILDECRAVLERGAREITLLGQNVNAFGQDKSGDGTSFAQLLRQVAGLPGLERLRYVT
PHPKDMGPEDVAAFAELPQLCPRLHLPLQAGSDVVLRSMRRRYDSAAFLRLVDDLRAARPDLALSTDLIVAFPGETEEDF
QDTLAMMRACNFMSSFSFIYSDRPGARAALFPGKIAPDVAQDRLRRLQALQDDLGARWLAARVENRDKTTLLMEGPSPKP
GESAEPSWQGRDPYGVPVHVPLPAGADHTGFLVQTVITEAKKHSLLAVRTGEPW

Sequences:

>Translated_454_residues
MIEKSYHIITFGCQMNVHDSQWLGRALTARGFVEASLEDAQVVVVNTCSVREKPEQKVMSALGRIRHVTGGNPDVLVGVA
GCVAQQLGEGFFSKESQVRLVAGSDGISGAPAAIERLLEEPTLHLSLLDFTSHYIEREAGPGTSGSSSGPVAFVNIMQGC
DNFCAYCIVPFTRGRQKSRSAAAILDECRAVLERGAREITLLGQNVNAFGQDKSGDGTSFAQLLRQVAGLPGLERLRYVT
PHPKDMGPEDVAAFAELPQLCPRLHLPLQAGSDVVLRSMRRRYDSAAFLRLVDDLRAARPDLALSTDLIVAFPGETEEDF
QDTLAMMRACNFMSSFSFIYSDRPGARAALFPGKIAPDVAQDRLRRLQALQDDLGARWLAARVENRDKTTLLMEGPSPKP
GESAEPSWQGRDPYGVPVHVPLPAGADHTGFLVQTVITEAKKHSLLAVRTGEPW
>Mature_454_residues
MIEKSYHIITFGCQMNVHDSQWLGRALTARGFVEASLEDAQVVVVNTCSVREKPEQKVMSALGRIRHVTGGNPDVLVGVA
GCVAQQLGEGFFSKESQVRLVAGSDGISGAPAAIERLLEEPTLHLSLLDFTSHYIEREAGPGTSGSSSGPVAFVNIMQGC
DNFCAYCIVPFTRGRQKSRSAAAILDECRAVLERGAREITLLGQNVNAFGQDKSGDGTSFAQLLRQVAGLPGLERLRYVT
PHPKDMGPEDVAAFAELPQLCPRLHLPLQAGSDVVLRSMRRRYDSAAFLRLVDDLRAARPDLALSTDLIVAFPGETEEDF
QDTLAMMRACNFMSSFSFIYSDRPGARAALFPGKIAPDVAQDRLRRLQALQDDLGARWLAARVENRDKTTLLMEGPSPKP
GESAEPSWQGRDPYGVPVHVPLPAGADHTGFLVQTVITEAKKHSLLAVRTGEPW

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=400, Percent_Identity=36, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI28872784, Length=400, Percent_Identity=36, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI93277076, Length=350, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1786882, Length=401, Percent_Identity=38.4039900249377, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1787057, Length=393, Percent_Identity=29.5165394402036, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=380, Percent_Identity=35.7894736842105, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=203, Percent_Identity=32.0197044334975, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356207, Length=463, Percent_Identity=34.7732181425486, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI19922432, Length=373, Percent_Identity=29.4906166219839, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49349; Mature: 49349

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKSYHIITFGCQMNVHDSQWLGRALTARGFVEASLEDAQVVVVNTCSVREKPEQKVMS
CCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHH
ALGRIRHVTGGNPDVLVGVAGCVAQQLGEGFFSKESQVRLVAGSDGISGAPAAIERLLEE
HHHHHHCCCCCCCCEEEHHHHHHHHHHCCHHCCCCCCEEEEECCCCCCCCHHHHHHHHHC
PTLHLSLLDFTSHYIEREAGPGTSGSSSGPVAFVNIMQGCDNFCAYCIVPFTRGRQKSRS
CCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHH
AAAILDECRAVLERGAREITLLGQNVNAFGQDKSGDGTSFAQLLRQVAGLPGLERLRYVT
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCC
PHPKDMGPEDVAAFAELPQLCPRLHLPLQAGSDVVLRSMRRRYDSAAFLRLVDDLRAARP
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLALSTDLIVAFPGETEEDFQDTLAMMRACNFMSSFSFIYSDRPGARAALFPGKIAPDVA
CCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
QDRLRRLQALQDDLGARWLAARVENRDKTTLLMEGPSPKPGESAEPSWQGRDPYGVPVHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEE
PLPAGADHTGFLVQTVITEAKKHSLLAVRTGEPW
ECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCC
>Mature Secondary Structure
MIEKSYHIITFGCQMNVHDSQWLGRALTARGFVEASLEDAQVVVVNTCSVREKPEQKVMS
CCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHH
ALGRIRHVTGGNPDVLVGVAGCVAQQLGEGFFSKESQVRLVAGSDGISGAPAAIERLLEE
HHHHHHCCCCCCCCEEEHHHHHHHHHHCCHHCCCCCCEEEEECCCCCCCCHHHHHHHHHC
PTLHLSLLDFTSHYIEREAGPGTSGSSSGPVAFVNIMQGCDNFCAYCIVPFTRGRQKSRS
CCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHH
AAAILDECRAVLERGAREITLLGQNVNAFGQDKSGDGTSFAQLLRQVAGLPGLERLRYVT
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCC
PHPKDMGPEDVAAFAELPQLCPRLHLPLQAGSDVVLRSMRRRYDSAAFLRLVDDLRAARP
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLALSTDLIVAFPGETEEDFQDTLAMMRACNFMSSFSFIYSDRPGARAALFPGKIAPDVA
CCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
QDRLRRLQALQDDLGARWLAARVENRDKTTLLMEGPSPKPGESAEPSWQGRDPYGVPVHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEE
PLPAGADHTGFLVQTVITEAKKHSLLAVRTGEPW
ECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA