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The map label for this gene is glnB [H]

Identifier: 220904418

GI number: 220904418

Start: 1352156

End: 1352494

Strand: Direct

Name: glnB [H]

Synonym: Ddes_1148

Alternate gene names: 220904418

Gene position: 1352156-1352494 (Clockwise)

Preceding gene: 220904417

Following gene: 220904419

Centisome position: 47.06

GC content: 50.74

Gene sequence:

>339_bases
ATGAAGAAGCTCGAGATAATTATTCGTCCCGGTATGTTTGAAAAAGTCAGGGATGTGCTCTCAGAACTGGGCATACACGG
ATTGAACTACACAGAAATAAAAGGATTCGGGCGGCAACGCGGCCATACTGAAGTGTACCGCGGCGCTATAATGCAGGTGG
ACTGCTTGCCCAAGATAAAAATTGAAATGGTTTTGCATGACAACATGCTTGAACCGGCAGTGAACGCGGTGATGCTCACT
GCACGCACAGGGCAGGTGGGAGACGGAAAGATTTTTATCAGCGCTGTGGAAGATGCCGTGCGCATCCGCACAGGTCAACG
TGGTGATGCGGCCCTGTAG

Upstream 100 bases:

>100_bases
GGTCGGATTGCGGGTTGCCCCCGAGGAAGAGTACAGCGGCCTTGACCTCGGCGAACACAATGAGCGCGGTTATAATCTGT
AATCCGGGAGAGACTGTGTC

Downstream 100 bases:

>100_bases
GCCGGAAATTTTAACGGCAAAGCTTCACGTATCCAGGTGATAAAGGAGAAAAGGCATGAGTTCCGTCCGCAAAAAAGCTT
TATTCATGGCCATTAATACC

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: PII signal transducing protein [H]

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKKLEIIIRPGMFEKVRDVLSELGIHGLNYTEIKGFGRQRGHTEVYRGAIMQVDCLPKIKIEMVLHDNMLEPAVNAVMLT
ARTGQVGDGKIFISAVEDAVRIRTGQRGDAAL

Sequences:

>Translated_112_residues
MKKLEIIIRPGMFEKVRDVLSELGIHGLNYTEIKGFGRQRGHTEVYRGAIMQVDCLPKIKIEMVLHDNMLEPAVNAVMLT
ARTGQVGDGKIFISAVEDAVRIRTGQRGDAAL
>Mature_112_residues
MKKLEIIIRPGMFEKVRDVLSELGIHGLNYTEIKGFGRQRGHTEVYRGAIMQVDCLPKIKIEMVLHDNMLEPAVNAVMLT
ARTGQVGDGKIFISAVEDAVRIRTGQRGDAAL

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=52.6785714285714, Blast_Score=129, Evalue=4e-32,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=49.1071428571429, Blast_Score=120, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12448; Mature: 12448

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLEIIIRPGMFEKVRDVLSELGIHGLNYTEIKGFGRQRGHTEVYRGAIMQVDCLPKIK
CCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHCCEEEECCCCCEE
IEMVLHDNMLEPAVNAVMLTARTGQVGDGKIFISAVEDAVRIRTGQRGDAAL
EEEEECCCCCCHHCCEEEEEECCCCCCCCEEEEEEHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKLEIIIRPGMFEKVRDVLSELGIHGLNYTEIKGFGRQRGHTEVYRGAIMQVDCLPKIK
CCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHCCEEEECCCCCEE
IEMVLHDNMLEPAVNAVMLTARTGQVGDGKIFISAVEDAVRIRTGQRGDAAL
EEEEECCCCCCHHCCEEEEEECCCCCCCCEEEEEEHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7921264 [H]