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The map label for this gene is lysC [H]

Identifier: 220904411

GI number: 220904411

Start: 1342929

End: 1344164

Strand: Reverse

Name: lysC [H]

Synonym: Ddes_1141

Alternate gene names: 220904411

Gene position: 1344164-1342929 (Counterclockwise)

Preceding gene: 220904412

Following gene: 220904410

Centisome position: 46.78

GC content: 53.4

Gene sequence:

>1236_bases
ATGGATACAGGCATGAAAATTTTGGTGCAAAAGTTTGGCGGCACGTCGGTAGCCAAGCTTGAATCTATGAAGCAGGTGCG
GGAAAAGGTTCTGCACGGTCTGGAAAAGGGCTATAAGGTTATAGCCGTACTGTCGGCCAGAGCCGGCGAAACCAACCAGC
TTCTTGCTCTGGCCGGCGAATGGTCGGCCACCCCCGACAGTGCGGAATGCGATGCCCTTGTGGCCACCGGCGAACAGGTT
TCCATCAGCCTGTTCACCATGCTGCTCAAGGATGCCGGCATTCGCGCCCGGTCTTTGCTGGGCTGGCAGATTCCCATTAC
AACCGATGATGATTTTGGCCGCGCGCGTATTCTTTCCATCAACAGCAAGGCCTTGTATGGCTTTCTGAAAGATCATGACG
TGCTGGTAGTTGCAGGTTTTCAGGGCTGTACTGAAAACGGCCGTATCACGACTCTCGGACGGGGTGGATCAGACACTTCG
GCAGTGGCTCTTGCTGCGGCCATGGGTTCCGCCGAATGCGAAATCTACACAGATGTGGATGGTGTTTACACCACAGACCC
CAACATCTGCTCCACCGCCCGAAAAATGGACCGTGTGGCCTATGAAGAAATGCTGGAAATGGCCAGCATGGGGGCCAAGG
TTCTGCACATCCGCTCAGTAGAATTTGCCAAAAAATATAAAGTTCCCGTACGGGTACGCTCCACATTCAGTAGTGATCCC
GGCACTCTCGTTACTCAGGAGGATTCCACAATGGAAGCCGTTCTTGTTTCCGGCATCGCCTATGATAAAGATCAAGCCCG
CGTAACCATTCGCGATCTGCCGGATGTACCGGGAATGGCCGCCGCCGTATTCGGTCCGCTTTCCGAAAAAGGCATCCTTG
TTGATATGATCGTGCAGAACACGAGCCTGGATGGCCATACAGACATTACCTTTACCATCTCGCGTAAAGACCTGCAGCAA
ACGCTCGAAGTGATGGAAGCTGTGGCCGAAAAGACCGGTGCCTCAGAAATTCTGCACGACGTAAGTGTGGCAAAGGTGTC
CGCCATCGGTGTGGGCATGCGCAACCATTCCGGAGTTGCTGCCCGCACCTTCGCTGCCCTCACTCATGAAGGCATCAATA
TCCTCATGATAAGCACCTCTGAAATCAAGATCACAATCCTGATTCAGGAAAAATACGTTGAACTGGCCGTCCGCATCCTG
CACGATACCTTCGGGCTTGATTGGGACATTGGCTAA

Upstream 100 bases:

>100_bases
TGCGCCATTTGTGCGAGCAATGGACTGTAAGCAAAGTCTGAACGCGACAGAACTGCGTTCGCGCAATTGGCCTGTTGTAC
ACTGTGAAGGATTGTGGGCA

Downstream 100 bases:

>100_bases
TCCCCCTCATATTTGAAAACCCGGCCACGGTAGCATAGTACCGTGGCCGGCCATATAGGGTATTTTTAGCCATGGCGTCA
GTAAAAATAACTGTTGCCGA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MDTGMKILVQKFGGTSVAKLESMKQVREKVLHGLEKGYKVIAVLSARAGETNQLLALAGEWSATPDSAECDALVATGEQV
SISLFTMLLKDAGIRARSLLGWQIPITTDDDFGRARILSINSKALYGFLKDHDVLVVAGFQGCTENGRITTLGRGGSDTS
AVALAAAMGSAECEIYTDVDGVYTTDPNICSTARKMDRVAYEEMLEMASMGAKVLHIRSVEFAKKYKVPVRVRSTFSSDP
GTLVTQEDSTMEAVLVSGIAYDKDQARVTIRDLPDVPGMAAAVFGPLSEKGILVDMIVQNTSLDGHTDITFTISRKDLQQ
TLEVMEAVAEKTGASEILHDVSVAKVSAIGVGMRNHSGVAARTFAALTHEGINILMISTSEIKITILIQEKYVELAVRIL
HDTFGLDWDIG

Sequences:

>Translated_411_residues
MDTGMKILVQKFGGTSVAKLESMKQVREKVLHGLEKGYKVIAVLSARAGETNQLLALAGEWSATPDSAECDALVATGEQV
SISLFTMLLKDAGIRARSLLGWQIPITTDDDFGRARILSINSKALYGFLKDHDVLVVAGFQGCTENGRITTLGRGGSDTS
AVALAAAMGSAECEIYTDVDGVYTTDPNICSTARKMDRVAYEEMLEMASMGAKVLHIRSVEFAKKYKVPVRVRSTFSSDP
GTLVTQEDSTMEAVLVSGIAYDKDQARVTIRDLPDVPGMAAAVFGPLSEKGILVDMIVQNTSLDGHTDITFTISRKDLQQ
TLEVMEAVAEKTGASEILHDVSVAKVSAIGVGMRNHSGVAARTFAALTHEGINILMISTSEIKITILIQEKYVELAVRIL
HDTFGLDWDIG
>Mature_411_residues
MDTGMKILVQKFGGTSVAKLESMKQVREKVLHGLEKGYKVIAVLSARAGETNQLLALAGEWSATPDSAECDALVATGEQV
SISLFTMLLKDAGIRARSLLGWQIPITTDDDFGRARILSINSKALYGFLKDHDVLVVAGFQGCTENGRITTLGRGGSDTS
AVALAAAMGSAECEIYTDVDGVYTTDPNICSTARKMDRVAYEEMLEMASMGAKVLHIRSVEFAKKYKVPVRVRSTFSSDP
GTLVTQEDSTMEAVLVSGIAYDKDQARVTIRDLPDVPGMAAAVFGPLSEKGILVDMIVQNTSLDGHTDITFTISRKDLQQ
TLEVMEAVAEKTGASEILHDVSVAKVSAIGVGMRNHSGVAARTFAALTHEGINILMISTSEIKITILIQEKYVELAVRIL
HDTFGLDWDIG

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=359, Percent_Identity=32.5905292479109, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1790455, Length=458, Percent_Identity=29.9126637554585, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1790376, Length=124, Percent_Identity=33.0645161290323, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320893, Length=365, Percent_Identity=26.027397260274, Blast_Score=109, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44239; Mature: 44239

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGMKILVQKFGGTSVAKLESMKQVREKVLHGLEKGYKVIAVLSARAGETNQLLALAGE
CCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECC
WSATPDSAECDALVATGEQVSISLFTMLLKDAGIRARSLLGWQIPITTDDDFGRARILSI
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHEEEEEEEECCCCCCCCEEEEEE
NSKALYGFLKDHDVLVVAGFQGCTENGRITTLGRGGSDTSAVALAAAMGSAECEIYTDVD
CCHHEEEEECCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCC
GVYTTDPNICSTARKMDRVAYEEMLEMASMGAKVLHIRSVEFAKKYKVPVRVRSTFSSDP
CEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHCCCCEEEEECCCCCC
GTLVTQEDSTMEAVLVSGIAYDKDQARVTIRDLPDVPGMAAAVFGPLSEKGILVDMIVQN
CEEEECCCCHHHHHHHHCCEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCEEEEEEEEC
TSLDGHTDITFTISRKDLQQTLEVMEAVAEKTGASEILHDVSVAKVSAIGVGMRNHSGVA
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCH
ARTFAALTHEGINILMISTSEIKITILIQEKYVELAVRILHDTFGLDWDIG
HHHHHHHHHCCCEEEEEECCCEEEEEEEEHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDTGMKILVQKFGGTSVAKLESMKQVREKVLHGLEKGYKVIAVLSARAGETNQLLALAGE
CCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECC
WSATPDSAECDALVATGEQVSISLFTMLLKDAGIRARSLLGWQIPITTDDDFGRARILSI
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHEEEEEEEECCCCCCCCEEEEEE
NSKALYGFLKDHDVLVVAGFQGCTENGRITTLGRGGSDTSAVALAAAMGSAECEIYTDVD
CCHHEEEEECCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCC
GVYTTDPNICSTARKMDRVAYEEMLEMASMGAKVLHIRSVEFAKKYKVPVRVRSTFSSDP
CEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHCCCCEEEEECCCCCC
GTLVTQEDSTMEAVLVSGIAYDKDQARVTIRDLPDVPGMAAAVFGPLSEKGILVDMIVQN
CEEEECCCCHHHHHHHHCCEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCEEEEEEEEC
TSLDGHTDITFTISRKDLQQTLEVMEAVAEKTGASEILHDVSVAKVSAIGVGMRNHSGVA
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCH
ARTFAALTHEGINILMISTSEIKITILIQEKYVELAVRILHDTFGLDWDIG
HHHHHHHHHCCCEEEEEECCCEEEEEEEEHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]