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The map label for this gene is gph [C]

Identifier: 220904409

GI number: 220904409

Start: 1340677

End: 1341330

Strand: Direct

Name: gph [C]

Synonym: Ddes_1139

Alternate gene names: 220904409

Gene position: 1340677-1341330 (Clockwise)

Preceding gene: 220904408

Following gene: 220904415

Centisome position: 46.66

GC content: 51.07

Gene sequence:

>654_bases
TTGAATTTTACCCCTGTTTTTTTTGATCTGGACGGAACCATCACGGATTCTGAAGATGGAATAGTCAACTCTGTGCAGTA
TGCGCTGGAACACTTTGGTGTTTCCTTACCGCGGGAAAGCCTGATTCCTTTTATCGGGCCTCCTCTGCGCGATTCATTTT
CAAAAATTTTCCCCAATGATCCGAAAAAAGTGGAAAAGATCGTTGGTAAGTACAGAGAATATTACACTGACCGGGGTATT
TTCGAAAACCGTCTTTATGACGGCATAAGCGATCTTTTGCGCGATCTGGCAGAAGAGGGGCGTGTTCTGGCGCTTGCCAC
TTCCAAGCCCGAAACGTTTGCCCTGCGTATTGTGGAGCACTTTGACATAGCACGCTACTTTTCGTGTGTCGCCGGGGCAG
AACTGAGTGGGCCGCGTAATAACAAGCCGGCTGTATTGCGCTACGCTTGCGATCGCCTGGGTTTGGTTCCTTCTGCATCC
TGCCTGATGGTCGGGGACCGCAAGTATGATGTAGCCGGTGCGCATGAGGTAGGGATGCCGTGTGCCGCGGTTTTATACGG
ATATGGCCCGGAAAAGGAACTGAAAGAGGCCGGGGCCGATTGGTTGTGCCCTGACGTGAAATCCTTGCGGGTCCTTTTGC
AGCAACGGAATTGA

Upstream 100 bases:

>100_bases
GAATGTGCAGGTTTTGATTCGTGCGATACAAGGCCGCCCCGTATTCGGGGCGGCCTTTGCAACAAAAGGCAGCATGGCTG
TCCGTCAAAGGAGCGCTCTC

Downstream 100 bases:

>100_bases
AGAAAAAAGTGCAGAGCAGGCTGTGCTGCCTCTCAGCTTTTTTCAGGCTGGGTACTGCGGGGTTTTATGTCCCGGCTATT
TTAGAGCAGTTAACGCTTCA

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: Nucleoside 5'-monophosphate phosphohydrolase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNFTPVFFDLDGTITDSEDGIVNSVQYALEHFGVSLPRESLIPFIGPPLRDSFSKIFPNDPKKVEKIVGKYREYYTDRGI
FENRLYDGISDLLRDLAEEGRVLALATSKPETFALRIVEHFDIARYFSCVAGAELSGPRNNKPAVLRYACDRLGLVPSAS
CLMVGDRKYDVAGAHEVGMPCAAVLYGYGPEKELKEAGADWLCPDVKSLRVLLQQRN

Sequences:

>Translated_217_residues
MNFTPVFFDLDGTITDSEDGIVNSVQYALEHFGVSLPRESLIPFIGPPLRDSFSKIFPNDPKKVEKIVGKYREYYTDRGI
FENRLYDGISDLLRDLAEEGRVLALATSKPETFALRIVEHFDIARYFSCVAGAELSGPRNNKPAVLRYACDRLGLVPSAS
CLMVGDRKYDVAGAHEVGMPCAAVLYGYGPEKELKEAGADWLCPDVKSLRVLLQQRN
>Mature_217_residues
MNFTPVFFDLDGTITDSEDGIVNSVQYALEHFGVSLPRESLIPFIGPPLRDSFSKIFPNDPKKVEKIVGKYREYYTDRGI
FENRLYDGISDLLRDLAEEGRVLALATSKPETFALRIVEHFDIARYFSCVAGAELSGPRNNKPAVLRYACDRLGLVPSAS
CLMVGDRKYDVAGAHEVGMPCAAVLYGYGPEKELKEAGADWLCPDVKSLRVLLQQRN

Specific function: Specifically dephosphorylates nucleoside 5'- monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=213, Percent_Identity=29.1079812206573, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 24186; Mature: 24186

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTPVFFDLDGTITDSEDGIVNSVQYALEHFGVSLPRESLIPFIGPPLRDSFSKIFPND
CCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHCCCC
PKKVEKIVGKYREYYTDRGIFENRLYDGISDLLRDLAEEGRVLALATSKPETFALRIVEH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
FDIARYFSCVAGAELSGPRNNKPAVLRYACDRLGLVPSASCLMVGDRKYDVAGAHEVGMP
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHCCH
CAAVLYGYGPEKELKEAGADWLCPDVKSLRVLLQQRN
HHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNFTPVFFDLDGTITDSEDGIVNSVQYALEHFGVSLPRESLIPFIGPPLRDSFSKIFPND
CCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHCCCC
PKKVEKIVGKYREYYTDRGIFENRLYDGISDLLRDLAEEGRVLALATSKPETFALRIVEH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
FDIARYFSCVAGAELSGPRNNKPAVLRYACDRLGLVPSASCLMVGDRKYDVAGAHEVGMP
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHCCH
CAAVLYGYGPEKELKEAGADWLCPDVKSLRVLLQQRN
HHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]