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The map label for this gene is cbiK [H]

Identifier: 220904399

GI number: 220904399

Start: 1329446

End: 1330228

Strand: Direct

Name: cbiK [H]

Synonym: Ddes_1129

Alternate gene names: 220904399

Gene position: 1329446-1330228 (Clockwise)

Preceding gene: 220904396

Following gene: 220904400

Centisome position: 46.27

GC content: 59.26

Gene sequence:

>783_bases
ATGAGACGTGCAATATTATTGGTAGCTTTTGGCGCAAGCAGCGCACAGGGGCAGAATGCCCTCAAAGGGTTTGATGCGCT
TGTGCGCCAGCGCTACCCGGGAATACCCGTGCGCTGGGCATATACCTCACTGCTGTTGCGGGAACGCCTGGCCCGAGCCA
GACAGAAGAGCGATTCCGTCCGCAAGGCCTTGAGGCGTCTCAGCCTGGAACGCTTTACCCATGTGGCGGTCCAGCCCTTG
CAAACCATACCGGGGCGGGAACACGGCGAAGTTTGCGCAGCTGTGGCTGAAATAGTAGCCCAGAGCGGTCTGCGCTGCCA
TGTTGGTGATCCCATGCTGGCATCTGCGGATGATGTAAACGCCGCTGCCCATGCCTGCGTGAACCATCTGCCATCCGGGC
GTACGGTTCATGAGGATGTCATTTTTATGGGCCACGGCGCAGAGCATCAGGCCGTGGCCCGTTACGCCGACCTGGCGGAA
GCCGTTTATGGCATTGACGCCCACGTACATGTGGGAGCCATGAACGGAGCTGTGTTGCTTGAGGATATTTTGCCCCGTCT
TGCTTCCGAGAGGGTGTGGCTCATGCCGCTGCTTTCAGTGGTTGGCAATCACGCGCTTAAGGATATGGCGGGGCAGGACA
GCCAGTCGTGGCGCAGCCGTGTGGAATCGCAGGGACGCCAGTGCGTGCCGGTGTTGAAAGGTACGGCGGAATACGCCGGT
TTTGCTGAGATATGGTTGCGCCACCTGGAGGCTGCCGCCCAGTCCTGTTTTGAATCTGTATGA

Upstream 100 bases:

>100_bases
TGTATCGTTGTGAGTCATGCTGGTCAATACAACGCTTTTCCCGTACTGCGGGCGACTGCCGTCTTGCCACCATGCTGCAA
GTAACGTACAAATTAGCCGC

Downstream 100 bases:

>100_bases
GGTAATTTATGCGCGGATTATTGATGACCATGCTGTGTGCGGCGATGCTGCTTGCCGGCTGTGGGGCTGGAAGCGGCGGC
CATGCCGCCGGAGGCGCTGT

Product: anaerobic cobalt chelatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MRRAILLVAFGASSAQGQNALKGFDALVRQRYPGIPVRWAYTSLLLRERLARARQKSDSVRKALRRLSLERFTHVAVQPL
QTIPGREHGEVCAAVAEIVAQSGLRCHVGDPMLASADDVNAAAHACVNHLPSGRTVHEDVIFMGHGAEHQAVARYADLAE
AVYGIDAHVHVGAMNGAVLLEDILPRLASERVWLMPLLSVVGNHALKDMAGQDSQSWRSRVESQGRQCVPVLKGTAEYAG
FAEIWLRHLEAAAQSCFESV

Sequences:

>Translated_260_residues
MRRAILLVAFGASSAQGQNALKGFDALVRQRYPGIPVRWAYTSLLLRERLARARQKSDSVRKALRRLSLERFTHVAVQPL
QTIPGREHGEVCAAVAEIVAQSGLRCHVGDPMLASADDVNAAAHACVNHLPSGRTVHEDVIFMGHGAEHQAVARYADLAE
AVYGIDAHVHVGAMNGAVLLEDILPRLASERVWLMPLLSVVGNHALKDMAGQDSQSWRSRVESQGRQCVPVLKGTAEYAG
FAEIWLRHLEAAAQSCFESV
>Mature_260_residues
MRRAILLVAFGASSAQGQNALKGFDALVRQRYPGIPVRWAYTSLLLRERLARARQKSDSVRKALRRLSLERFTHVAVQPL
QTIPGREHGEVCAAVAEIVAQSGLRCHVGDPMLASADDVNAAAHACVNHLPSGRTVHEDVIFMGHGAEHQAVARYADLAE
AVYGIDAHVHVGAMNGAVLLEDILPRLASERVWLMPLLSVVGNHALKDMAGQDSQSWRSRVESQGRQCVPVLKGTAEYAG
FAEIWLRHLEAAAQSCFESV

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 28337; Mature: 28337

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAILLVAFGASSAQGQNALKGFDALVRQRYPGIPVRWAYTSLLLRERLARARQKSDSV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RKALRRLSLERFTHVAVQPLQTIPGREHGEVCAAVAEIVAQSGLRCHVGDPMLASADDVN
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHH
AAAHACVNHLPSGRTVHEDVIFMGHGAEHQAVARYADLAEAVYGIDAHVHVGAMNGAVLL
HHHHHHHHHCCCCCEEHHCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
EDILPRLASERVWLMPLLSVVGNHALKDMAGQDSQSWRSRVESQGRQCVPVLKGTAEYAG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEHHHHHCCHHHCC
FAEIWLRHLEAAAQSCFESV
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRAILLVAFGASSAQGQNALKGFDALVRQRYPGIPVRWAYTSLLLRERLARARQKSDSV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RKALRRLSLERFTHVAVQPLQTIPGREHGEVCAAVAEIVAQSGLRCHVGDPMLASADDVN
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHH
AAAHACVNHLPSGRTVHEDVIFMGHGAEHQAVARYADLAEAVYGIDAHVHVGAMNGAVLL
HHHHHHHHHCCCCCEEHHCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
EDILPRLASERVWLMPLLSVVGNHALKDMAGQDSQSWRSRVESQGRQCVPVLKGTAEYAG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEHHHHHCCHHHCC
FAEIWLRHLEAAAQSCFESV
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]