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The map label for this gene is dacF [H]

Identifier: 220904314

GI number: 220904314

Start: 1218492

End: 1219268

Strand: Reverse

Name: dacF [H]

Synonym: Ddes_1041

Alternate gene names: 220904314

Gene position: 1219268-1218492 (Counterclockwise)

Preceding gene: 220904315

Following gene: 220904312

Centisome position: 42.43

GC content: 57.79

Gene sequence:

>777_bases
ATGCCCGTGCGTTCCGCCATTCTTATCAATATGGACACCGGCAAAATTCTTTATGCCAAAAATGCGGATATGGCCATTCC
CCCGGCTTCACTCACCAAGGTCATGACAATGTACCTAGCTATGGACCAGATTCGCGCGGGCAAGATCAAACCGGGTGAAA
AAATCAGGATTACGCCGCAGGTAGCTGCCGTAGGCGGATCATCCATGCACTTGCGGGCAGGCGAGCGCGTCGCGCTGTCA
CAGCTGCTTACGGGCATGGCCGTGGTTTCCGGCAATGATGCAGCCACAGCAGTGGCCCGCCGGGTTGGTGGCAATGACCG
CCAGTTTGTTCGCGCTATGAACCAGAAGGCCAAGGCCCTGGGTATGAACCGCACCACATTCAAAAACCCCACCGGGCTAC
CGGCCGCCGGACAGAAAACCACTGCCAGAGACATGGCGCGAATGACGAGAGCCTACCTGCGTTCTCACCCGTCTGCCCTG
CGCTACCACAGTGTGCAGCTGCTCACCTATCGGGACAGAAAAACGCGCAATACCAATGGGCTGCTGGGTACGGTCGCTGG
CGTCAACGGCTTGAAGACGGGCTGGACCGTAGCCTCGGGCTACAACCTTATTGTGACAGGTCAACGGGGTAAAACCCGCA
TGCTGGCAGTGGTTATGGGCGGCACCAGCAGAGTCGGGCGCGATAATGCCGCCCGCAGACTCATTGAGGCCGGATTCCGG
CATCCGGCATCGCCTAAAAAAGTACAGCAAATGTTCTCTGCCAGAAAAAAGCGCTGA

Upstream 100 bases:

>100_bases
CCACGGCGCACTACCGTGGGCGACTGCACGTCTGGCGGTCTGTTTGTTGTGGCTTTGCCTGATTGCCATCTGTGCAGTTC
CTGGCGGCGCTCAGGCGGCC

Downstream 100 bases:

>100_bases
CGCCAGCCATGCCAGAGCACATTATAATTTTTTGTGGCTGAAGCAGGGAAGCGGCAATGCATGTGGCGCAGCCTGTTACA
CAAAAAATCAGTTAAAAGCA

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPVRSAILINMDTGKILYAKNADMAIPPASLTKVMTMYLAMDQIRAGKIKPGEKIRITPQVAAVGGSSMHLRAGERVALS
QLLTGMAVVSGNDAATAVARRVGGNDRQFVRAMNQKAKALGMNRTTFKNPTGLPAAGQKTTARDMARMTRAYLRSHPSAL
RYHSVQLLTYRDRKTRNTNGLLGTVAGVNGLKTGWTVASGYNLIVTGQRGKTRMLAVVMGGTSRVGRDNAARRLIEAGFR
HPASPKKVQQMFSARKKR

Sequences:

>Translated_258_residues
MPVRSAILINMDTGKILYAKNADMAIPPASLTKVMTMYLAMDQIRAGKIKPGEKIRITPQVAAVGGSSMHLRAGERVALS
QLLTGMAVVSGNDAATAVARRVGGNDRQFVRAMNQKAKALGMNRTTFKNPTGLPAAGQKTTARDMARMTRAYLRSHPSAL
RYHSVQLLTYRDRKTRNTNGLLGTVAGVNGLKTGWTVASGYNLIVTGQRGKTRMLAVVMGGTSRVGRDNAARRLIEAGFR
HPASPKKVQQMFSARKKR
>Mature_257_residues
PVRSAILINMDTGKILYAKNADMAIPPASLTKVMTMYLAMDQIRAGKIKPGEKIRITPQVAAVGGSSMHLRAGERVALSQ
LLTGMAVVSGNDAATAVARRVGGNDRQFVRAMNQKAKALGMNRTTFKNPTGLPAAGQKTTARDMARMTRAYLRSHPSALR
YHSVQLLTYRDRKTRNTNGLLGTVAGVNGLKTGWTVASGYNLIVTGQRGKTRMLAVVMGGTSRVGRDNAARRLIEAGFRH
PASPKKVQQMFSARKKR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=261, Percent_Identity=37.9310344827586, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI87082022, Length=241, Percent_Identity=39.0041493775934, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1787062, Length=245, Percent_Identity=37.5510204081633, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI87082054, Length=251, Percent_Identity=29.0836653386454, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 27920; Mature: 27789

Theoretical pI: Translated: 12.24; Mature: 12.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVRSAILINMDTGKILYAKNADMAIPPASLTKVMTMYLAMDQIRAGKIKPGEKIRITPQ
CCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
VAAVGGSSMHLRAGERVALSQLLTGMAVVSGNDAATAVARRVGGNDRQFVRAMNQKAKAL
EEECCCCEEEECCCCHHHHHHHHHHHEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GMNRTTFKNPTGLPAAGQKTTARDMARMTRAYLRSHPSALRYHSVQLLTYRDRKTRNTNG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEECCCCCCCCCC
LLGTVAGVNGLKTGWTVASGYNLIVTGQRGKTRMLAVVMGGTSRVGRDNAARRLIEAGFR
CEEEECCCCCCCCCCEEECCCEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCC
HPASPKKVQQMFSARKKR
CCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVRSAILINMDTGKILYAKNADMAIPPASLTKVMTMYLAMDQIRAGKIKPGEKIRITPQ
CCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
VAAVGGSSMHLRAGERVALSQLLTGMAVVSGNDAATAVARRVGGNDRQFVRAMNQKAKAL
EEECCCCEEEECCCCHHHHHHHHHHHEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GMNRTTFKNPTGLPAAGQKTTARDMARMTRAYLRSHPSALRYHSVQLLTYRDRKTRNTNG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEECCCCCCCCCC
LLGTVAGVNGLKTGWTVASGYNLIVTGQRGKTRMLAVVMGGTSRVGRDNAARRLIEAGFR
CEEEECCCCCCCCCCEEECCCEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCC
HPASPKKVQQMFSARKKR
CCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]