Click here to switch to the map view.

The map label for this gene is nikC [H]

Identifier: 220904303

GI number: 220904303

Start: 1205974

End: 1206786

Strand: Reverse

Name: nikC [H]

Synonym: Ddes_1030

Alternate gene names: 220904303

Gene position: 1206786-1205974 (Counterclockwise)

Preceding gene: 220904304

Following gene: 220904302

Centisome position: 42.0

GC content: 61.01

Gene sequence:

>813_bases
ATGAACAAAAAAACGCTCGTAGCCCTTCTCATGCTGCTGCCGCTGATGGCCTGCGCCCTGCTGGGTCCCTGGCTGGCGCC
CCACGACCCCGAAGCCGTGGAGCTGGCAAAAAAATTTCTGCCTCCCGATGCGGACCATCTGCTGGGCACAGACCACCTGG
GGCGCGACATTCTCTCCCGCCTCATGGTGGGGGCCCGCTACTCCCTGTCCACAGTGCTGTACATCATCGCCTGTATCGTG
GCGGCAAGCCTCGCCCTTGGCTCCGTGGCAGGCTATCTCGGCGGCAGGGCAGATTCCGCCATCATGCGCGTCTGCGATGC
CATGATGACCTTTCCAACCATAGTATTGGCGCTTTTTCTTATCGGCATTCTCGGGGCCGGGCTGACAAACCTTGTCATCG
CCATCGCTGTAACCCATGTGGCATGGTACACACGGCTCGTGCGCAGTCATGTACTGAGCCTGAAAAGCCGGGGGCATATC
CTGGCAGCGCAGGCCATGGGCCTGAGCCGCACACGCATTGTCATCCGCCATTTCCTGCCCGCGATTATTGTGCAGGTTCT
GGTACTTGCAACGCTGGATGTGGGACATATGCTTCTGCATGTGGCCGGGCTTTCCTTTCTGGGGCTGGGCATACAGCCCC
CCACACCGGAGTGGGGACTCATGATCTCCGAAGCCCGCAACTACATACGCTCGCAACCCATGCTCATGTTCTATCCCGGC
ATGATGATCTTCATGGCTACTGCCGCCTGCAACCTTATGGGCGATGCGCTGCGGGACCGCCTGGACCCTGCCCTGCGCGC
CGCGGGGGCCTGA

Upstream 100 bases:

>100_bases
CGTTTACACTGGTAACCACCCTTATTTTTGTGTTTGTCAACCTGCTTACTGATGTACTTTACGCGCGCATCGACCCGCGC
ATCCGCCTTGGAGAAGGACG

Downstream 100 bases:

>100_bases
TATGGAAGATGTGACAAAACCGGCCTGCCAGCCGAACTCCCCATACATTCTTGAGGTTCGCGACCTTTGCCTGAAAACAG
CCGGAACAGAAGCATCAAGG

Product: nickel transporter permease NikC

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNKKTLVALLMLLPLMACALLGPWLAPHDPEAVELAKKFLPPDADHLLGTDHLGRDILSRLMVGARYSLSTVLYIIACIV
AASLALGSVAGYLGGRADSAIMRVCDAMMTFPTIVLALFLIGILGAGLTNLVIAIAVTHVAWYTRLVRSHVLSLKSRGHI
LAAQAMGLSRTRIVIRHFLPAIIVQVLVLATLDVGHMLLHVAGLSFLGLGIQPPTPEWGLMISEARNYIRSQPMLMFYPG
MMIFMATAACNLMGDALRDRLDPALRAAGA

Sequences:

>Translated_270_residues
MNKKTLVALLMLLPLMACALLGPWLAPHDPEAVELAKKFLPPDADHLLGTDHLGRDILSRLMVGARYSLSTVLYIIACIV
AASLALGSVAGYLGGRADSAIMRVCDAMMTFPTIVLALFLIGILGAGLTNLVIAIAVTHVAWYTRLVRSHVLSLKSRGHI
LAAQAMGLSRTRIVIRHFLPAIIVQVLVLATLDVGHMLLHVAGLSFLGLGIQPPTPEWGLMISEARNYIRSQPMLMFYPG
MMIFMATAACNLMGDALRDRLDPALRAAGA
>Mature_270_residues
MNKKTLVALLMLLPLMACALLGPWLAPHDPEAVELAKKFLPPDADHLLGTDHLGRDILSRLMVGARYSLSTVLYIIACIV
AASLALGSVAGYLGGRADSAIMRVCDAMMTFPTIVLALFLIGILGAGLTNLVIAIAVTHVAWYTRLVRSHVLSLKSRGHI
LAAQAMGLSRTRIVIRHFLPAIIVQVLVLATLDVGHMLLHVAGLSFLGLGIQPPTPEWGLMISEARNYIRSQPMLMFYPG
MMIFMATAACNLMGDALRDRLDPALRAAGA

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789889, Length=242, Percent_Identity=54.1322314049587, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI1787054, Length=262, Percent_Identity=39.3129770992366, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI1787760, Length=263, Percent_Identity=38.7832699619772, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789964, Length=272, Percent_Identity=37.8676470588235, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787498, Length=271, Percent_Identity=36.5313653136531, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787549, Length=263, Percent_Identity=28.1368821292776, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788505, Length=229, Percent_Identity=29.2576419213974, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014157 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29038; Mature: 29038

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]