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The map label for this gene is hemK [H]

Identifier: 21910336

GI number: 21910336

Start: 851664

End: 852503

Strand: Direct

Name: hemK [H]

Synonym: SpyM3_0800

Alternate gene names: 21910336

Gene position: 851664-852503 (Clockwise)

Preceding gene: 21910335

Following gene: 21910337

Centisome position: 44.81

GC content: 35.71

Gene sequence:

>840_bases
ATGAACTATGCTACATTAATCAGAACTTATGAAGATAAACTAGAACAAATAGATGAGGATCGCGAAAATCTCGCCTATGT
TTTTAGAGAAATCAAAGAATGGTCAAGTCTAGATATGTTGATTCACCAAAATCAAGCTGTGACTCCTGAAGATGCTGTTC
TTTTGGAACATATTTTCTGTTCTCTAAGTCAGCATTTATCTCCTCAATATATTACAGGTAATGCTTATTTTCGTGATTTG
AAGCTTGCAGTCGACAAGCGTGTATTAATTCCAAGGCCAGAAACAGAAGAATTAGTTGACATGATTTTAGCTGAAAATCT
TGATGCACCGTTGAATGTTTTAGATATTGGTACAGGTAGTGGAGCGATTGCTATTTCTCTCAAAAAAGAAAGGTCTAACT
GGCAAGTAACGGCATCTGATATTTCTAGAGCTGCTCTTGACTTGGCAAAAGCCAATGCTGATGCTTACCAGCTTGATATA
ACGTTTATTGAATCAGATGTTTTTAGTCTCATTTCAGGAACATTTGATATTATTGTGTCAAATCCTCCCTACATATCCTA
CGAGGATAAGGAAGAAGTTAGTCTAAATGTTTTGCAATCAGAGCCTCATTTGGCACTTTTTGCTAAAGAAAATGGCTATG
CTATTTACCGTAAGATTATTGAACAAGCTGACAATTACTTAGCTAAGGAGGGGAAGCTTTACTTTGAAATTGGTTATAAG
CAAGCTGAAGGTATAAAAGATATGTTACAAGCCTATTTTCCTCAAAGACATATACGAGTCGTAACGGATATATTTGGTAA
AGAAAGGATGGTGGTTGTTGATTATGGAGTATCTAGCTAG

Upstream 100 bases:

>100_bases
TTCAAAAATTAGATACTATTTTATCTGGAAAAATGGATGAAGTTATTGATGCACTCGTTATGTATGATCAGACAAAGAAA
TTAGAATCTCTCAATAACTA

Downstream 100 bases:

>100_bases
TATCATTGAATCAGGTGATGCCCTTGTTTTGCCAACAGAAACGGTTTATGGTCTTTTCGCCAAGGCTTTAGATGAAAAAG
CAGTAAATGCTGTTTATGAC

Product: putative protoporphyrinogen oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDL
KLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGSGAIAISLKKERSNWQVTASDISRAALDLAKANADAYQLDI
TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLAKEGKLYFEIGYK
QAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYGVSS

Sequences:

>Translated_279_residues
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDL
KLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGSGAIAISLKKERSNWQVTASDISRAALDLAKANADAYQLDI
TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLAKEGKLYFEIGYK
QAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYGVSS
>Mature_279_residues
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDL
KLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGSGAIAISLKKERSNWQVTASDISRAALDLAKANADAYQLDI
TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLAKEGKLYFEIGYK
QAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYGVSS

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=278, Percent_Identity=30.9352517985612, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1787463, Length=179, Percent_Identity=40.7821229050279, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI87082085, Length=189, Percent_Identity=34.3915343915344, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324266, Length=189, Percent_Identity=30.6878306878307, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582226, Length=284, Percent_Identity=27.8169014084507, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31742; Mature: 31742

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFC
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHH
SLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGS
HHHHCCCCCEEECCHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
GAIAISLKKERSNWQVTASDISRAALDLAKANADAYQLDITFIESDVFSLISGTFDIIVS
CEEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHCCCEEEEEC
NPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLAKEGKLYFEIGYK
CCCEECCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
QAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYGVSS
HHCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFC
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHH
SLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGS
HHHHCCCCCEEECCHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
GAIAISLKKERSNWQVTASDISRAALDLAKANADAYQLDITFIESDVFSLISGTFDIIVS
CEEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHCCCEEEEEC
NPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLAKEGKLYFEIGYK
CCCEECCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
QAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYGVSS
HHCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]