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The map label for this gene is iscS1 [H]

Identifier: 21910317

GI number: 21910317

Start: 834441

End: 835568

Strand: Reverse

Name: iscS1 [H]

Synonym: SpyM3_0781

Alternate gene names: 21910317

Gene position: 835568-834441 (Counterclockwise)

Preceding gene: 21910318

Following gene: 21910316

Centisome position: 43.97

GC content: 39.98

Gene sequence:

>1128_bases
ATGACTTACTTTGATAACGCCGCCACCACACCACTTAGTCCTAATGTGATTAGGGCGATGACAGCAGCTATGCAAGATAA
CTTTGGTAACCCCTCTAGTATTCATTTCTACGGGCGCCGAGCCAATAAAATCCTTCGTGAATGCCGACAAGCTATCGCTA
GAAACTTGGGGGCAAGTGAACAGCAAATTATAGTAACGTCCGGTGGAACTGAAAGCAATAATATGGCCATAAAAGGCTAT
GCTTTAGCTCATCAAGCAAAGGGCAAACATCTCATCACAACTACTATTGAGCACCACTCTGTGCTTCATACCATGGCCTA
TCTCGAAGAGCGATTTGGTTTTGAGGTCACTTATTTGCCTTGTCAAAATGGACAAATAAATTTATCTGACTTAAAACAAG
CCTTGAGAGATGACACCATTTTGGTAAGCATAATGTATGCCAATAACGAAACAGGTGACCTGTTACCTATTAAGGACATT
GGGAATCTGCTCAAAGACCATCAAGCTGCTTTTCATGTTGATGCCGTTCAAGCTGTTGGCAAACTCAAGATTATTCCTAG
CGAACTTGGGATTGACTTTTTATCTGCTTCTGCTCACAAATTTCATGGTCCTAAAGGATGCGGCTTTTTATACAGTAACG
GACAACCAATCGACCCTCTTCTTCACGGGGGTGATCAAGAAGGAAAGCGACGCGCAAGTACTGAAAATATGTTAGGTATT
ATTGGTATGGCACAAGCTTTGACTGATGCTATGACTTGTCTTGACCAATCAACTGATCACATTATCAGTTTACGTCATCA
CTTGATTAGCCTCCTAGAAGGACTACCCTACTATATTAATCAAGGTACTCATTATCTTCCTCATGTTCTCAATATTGGAT
TTTTAGGCTACCAAAATACTATTTTACTAACACAACTTGACTTAGCAGGCATTGCAGTATCAACTGGTTCTGCTTGTACT
GCTGGAGCTGTTAATCCAAGTCATGTTTTAGCTGCGTACTATGGGGATGACTCTTCTCGTTTAAAAGAATCAATTCGTAT
CAGTTTTTCAGATCAAAATAGCATTGAAGATGTTAATCAATTAGCTCAAACATTAAAAAATATTTTAGGAGGAACAGATG
GCTTTTGA

Upstream 100 bases:

>100_bases
TTAGCGCCAGTGATTTAATCGCAGAAGCTATTATTCGTATCCATGAAAGAAGACCCTTAAGTCCACTTTTTTCTTATCAA
CCTAAAGGCAAAAATAACGC

Downstream 100 bases:

>100_bases
AAAAGAAATTAAGCTTAAAGACTGTAAATACCTTTATACAATCAGTCCAAACATTAAAAAATATACCCTAAGAGATACTA
CATTTAGTCAAACTAAAGTA

Product: putative iron-sulfur cofactor synthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKILRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGY
ALAHQAKGKHLITTTIEHHSVLHTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPIDPLLHGGDQEGKRRASTENMLGI
IGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLPYYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACT
AGAVNPSHVLAAYYGDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF

Sequences:

>Translated_375_residues
MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKILRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGY
ALAHQAKGKHLITTTIEHHSVLHTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPIDPLLHGGDQEGKRRASTENMLGI
IGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLPYYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACT
AGAVNPSHVLAAYYGDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF
>Mature_374_residues
TYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKILRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYA
LAHQAKGKHLITTTIEHHSVLHTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDIG
NLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPIDPLLHGGDQEGKRRASTENMLGII
GMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLPYYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTA
GAVNPSHVLAAYYGDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=365, Percent_Identity=38.9041095890411, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=34.5498783454988, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI48994898, Length=377, Percent_Identity=40.053050397878, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1787970, Length=390, Percent_Identity=25.8974358974359, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789175, Length=395, Percent_Identity=24.8101265822785, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=368, Percent_Identity=39.6739130434783, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=30.2752293577982, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=373, Percent_Identity=37.5335120643432, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI20129463, Length=368, Percent_Identity=37.2282608695652, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40761; Mature: 40630

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKILRECRQAIARNLGASE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCC
QQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVLHTMAYLEERFGFEVTYLP
CEEEEEECCCCCCCEEEEEEEEEHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEEE
CQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDIGNLLKDHQAAFHVDAVQAVG
CCCCEEEHHHHHHHHCCCEEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
KLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPIDPLLHGGDQEGKRRASTENMLGI
CEEEEHHHHCCHHHHCCHHHCCCCCCCEEEECCCCCCCHHHCCCCHHHHHHHCHHHHHHH
IGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLPYYINQGTHYLPHVLNIGFLGYQNT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHCCEECCCCE
ILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYYGDDSSRLKESIRISFSDQNSIEDVNQ
EEEEEECEEEEEEECCCCCCCCCCCHHHEEEEEECCCHHHHHHHEEEEECCCCCHHHHHH
LAQTLKNILGGTDGF
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
TYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKILRECRQAIARNLGASE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCC
QQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVLHTMAYLEERFGFEVTYLP
CEEEEEECCCCCCCEEEEEEEEEHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEEE
CQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDIGNLLKDHQAAFHVDAVQAVG
CCCCEEEHHHHHHHHCCCEEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
KLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPIDPLLHGGDQEGKRRASTENMLGI
CEEEEHHHHCCHHHHCCHHHCCCCCCCEEEECCCCCCCHHHCCCCHHHHHHHCHHHHHHH
IGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLPYYINQGTHYLPHVLNIGFLGYQNT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHCCEECCCCE
ILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYYGDDSSRLKESIRISFSDQNSIEDVNQ
EEEEEECEEEEEEECCCCCCCCCCCHHHEEEEEECCCHHHHHHHEEEEECCCCCHHHHHH
LAQTLKNILGGTDGF
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]