Click here to switch to the map view.

The map label for this gene is aphA [H]

Identifier: 21910309

GI number: 21910309

Start: 827247

End: 827978

Strand: Direct

Name: aphA [H]

Synonym: SpyM3_0773

Alternate gene names: 21910309

Gene position: 827247-827978 (Clockwise)

Preceding gene: 21910307

Following gene: 21910310

Centisome position: 43.53

GC content: 36.07

Gene sequence:

>732_bases
ATGAAAAAAGAATTTACCAGTATCCTGTTTACAGTTAGTTTTTGTGGAATCATAGCATTGCCTGTCGAAGCTAGTGGCCC
TAAAGTTCCTTATACACAAGAAGGTATAACGGCCATTTCAAATCAGGCAACAGTAAAATTGATTTCTATTGCTGATATCG
CGAGCAGTTTAGAAGGACAAAAACCTATAACGGTTAGTTTTGATATCGATGATACTTTGTTATTTACTAGCCAATATTTT
CAGTACGGCAAAGAATATATAACTCCAGGCTCATTTGATTTTTTGCATAAGCAAAAATTTTGGGACTTAGTCGCTAAACG
GGGAGATCAGGATTCTATTCCAAAAGAGTATGCAAAGCAACTAATCGCAATGCATCAAAAACGAGGAGATAAAATTGTCT
TTATTACTGGCCGAACTCGAGGATCGATGTATAAAAAAGGTGAGATTGATAAAACAGCTAAGTCACTGGCTAAAGATTTT
AAGTTAGACAAGCCAATAGCTATCAATTATACAGGAGATAAAGCTGTGAAGCCGTATCAGTATGATAAAACATATTATAT
AAAGAAAAACGGCTCACAAATTCATTACGGAGATAGTGATGAAGATATAAATGCTGCCAAAGAGGCAGGTGCAAGACCTA
TTCGCATTTTAAGAGCTCCTAATTCAACCAATCTTCCTTTGCCAAAAGCTGGAGGATACGGAGAAGAGGTTTTAGAAAAT
TCGGCTTATTAG

Upstream 100 bases:

>100_bases
TCATCCTTATTCAGAACTTAAAGGGCACTATTATAAGAATTTGAAACCGCTTTACTTAAACCGTGTATTTTTGTACAATA
ATGATTAAGGAGGTTTTTAG

Downstream 100 bases:

>100_bases
TCAAATATTTGTGTAAGAAAGGAAGTAACAGTAATTTCTTTCATCACCCCACTGAGTAACCAATAGCCCAGTGGGTTTTT
TGGGTTACAGAAATCAGTTC

Product: acid phosphatase/phosphotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQATVKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYF
QYGKEYITPGSFDFLHKQKFWDLVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILRAPNSTNLPLPKAGGYGEEVLEN
SAY

Sequences:

>Translated_243_residues
MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQATVKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYF
QYGKEYITPGSFDFLHKQKFWDLVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILRAPNSTNLPLPKAGGYGEEVLEN
SAY
>Mature_243_residues
MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQATVKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYF
QYGKEYITPGSFDFLHKQKFWDLVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILRAPNSTNLPLPKAGGYGEEVLEN
SAY

Specific function: Dephosphorylates Several Organic Phosphomonoesters And Catalyzes The Transfer Of Low-Energy Phosphate Groups From Phosphomonoesters To Hydroxyl Groups Of Various Organic Compounds. Preferentially Acts On Aryl Phosphoesters. Maximal Activity At Ph 6-6.5 To

COG id: COG3700

COG function: function code R; Acid phosphatase (class B)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class B bacterial acid phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI2367341, Length=224, Percent_Identity=42.8571428571429, Blast_Score=173, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005519
- InterPro:   IPR023214
- InterPro:   IPR010025 [H]

Pfam domain/function: PF03767 Acid_phosphat_B [H]

EC number: =3.1.3.2 [H]

Molecular weight: Translated: 27087; Mature: 27087

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQATVKLISIADIASSLEGQ
CCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHHHHCCCCEEEEEEHHHHHHHCCCC
KPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWDLVAKRGDQDSIPKEYAKQ
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDFKLDKPIAINYTGDKAVKPYQ
HHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEE
YDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILRAPNSTNLPLPKAGGYGEEVLEN
CCCEEEEEECCCEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHC
SAY
CCC
>Mature Secondary Structure
MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQATVKLISIADIASSLEGQ
CCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHHHHCCCCEEEEEEHHHHHHHCCCC
KPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWDLVAKRGDQDSIPKEYAKQ
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDFKLDKPIAINYTGDKAVKPYQ
HHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEE
YDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILRAPNSTNLPLPKAGGYGEEVLEN
CCCEEEEEECCCEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHC
SAY
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]