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The map label for this gene is lpdA [H]

Identifier: 218930917

GI number: 218930917

Start: 4399164

End: 4400627

Strand: Direct

Name: lpdA [H]

Synonym: YPO3917

Alternate gene names: 218930917

Gene position: 4399164-4400627 (Clockwise)

Preceding gene: 218930916

Following gene: 218930926

Centisome position: 94.53

GC content: 51.71

Gene sequence:

>1464_bases
ATGAAAACCTTAAACGTTGATGTCGCTGTTATTGGCGGCGGAACGGCCGGACTGGGCGCTTATCGCGCGGCCAAACTGTT
GACCCCCAACGTAGTGATGATTGAAGGTGGTGAATATGGTACCACCTGCGCACGCGTCGGCTGCATGCCATCCAAACTAT
TGATTGCGGCGGCTGAAGCCGTCCACCAGATAGAAAAAGCGCCCGGATTTGGTATTCACCCACAAGGTAAGCCCCTGATT
AATGGCCGTGAAGTCATGGACCGCGTTAAGCGTGAACGTGACCGTTTTGTGGGCTTCGTATTGGAAAGCGTAGAGACCAT
TCCTGCGGCCGATAAAATTCAAGGTTATGCCCGTTTTATTGATGATAACACCCTGCAAGTTGATGATCACACGCAGCTTA
CTTCACAAAAAATCATTGCGCAGCGGATCGTGATTGCGACAGGTTCCCGCCCAAGCTGGCCCGCCTCTTGGAATGAGCTA
GGTGACCGGTTGATTATTAATGATGACGTCTTTAATTGGGACGATCTGCCAGAATCTGTGGCAGTTTTTGGCCCAGGCGT
TATTGGCTTAGAACTTGGTCAGGCACTGCACCGGTTAGGTGTCCAGGTAAAAATGTTTGGTGTCGGTGGTGCCGTTGGCC
CGCTGACCGACAGTATCGTGCGTGACTACGCAGCAAAAACGTTGGGTGATGAGTTCTATCTTGATCCCGATGTAAAAGTT
GAGCTGATGCAACGTGAGGGAGATAAAGTCTTTATCCGTTATCTGGATAAAAGCGGTAGGCCACAGGAGATTATGGTGGA
CTACGTCTTAGCCGCCACGGGCCGCCGCCCTAATGTAGATAAATTGGGGCTGGAGAATACGTCACTCATTCTGGATGAAC
GCGGAGTGCCGCAGGCAGACCGCCTGACCATGCAAACCAACGTGCCGCATATTTTTATTGCAGGTGATGCCAGCAATCAG
TTGCCATTACTGCATGAAGCCAGCGATCAGGCACGTATTGCCGGTGTAAATGCGGGAGGATTCCCTGAGGTTGTACCCGG
TTTACGCCGTAGCCCAATTTCAGTCGTGTTCTCTGACCCACAAATTGCCATGGTGGGGGCAACCTTCCGTGAGCTAGCAC
AAAAATTCAGCGCTTGTGGTTGCTTTGAGATTGGCGAAGTCTCCTTTGAGAATCAAGGTCGCTCGCGGGTGATGCTAAAA
AACAAAGGCATTTTACGCATTTATGGCGAACAGGGCACTGGCCGATTCTTAGGGGCAGAGATGATGGGGCCAAGTGCGGA
GCACATCGCCCACTTACTGGCATGGGCGCATCAGCAACAAATGACCATCGACCAGATGCTCGATATGCCGTTTTATCATC
CGGTCATTGAAGAAGGCCTGCGTACCGCATTGCGTGATTTGCAATCTAAGTTAAAGCTGGGCGCTGATGAAGCGGAGCGC
TGCCTACGCTGCCCTGGCGAATAA

Upstream 100 bases:

>100_bases
GCCTAATTATATTGATTAACAAGCATATTAATAAAAATTAAAGCAATAGGAAGTAACTTGGTAGGCTCCGGCCTACCTTT
TTTTTCTGTTAGGAGTAGAC

Downstream 100 bases:

>100_bases
GGCCTCAGGCGGTTCAAACCGGGCACGGCGCCCGGTTTTTAATCCCCCGTTCTCCCCTCTCTTATTTTGCGTACTTTGCG
CTCTACTCATATGTTACCCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEAVHQIEKAPGFGIHPQGKPLI
NGREVMDRVKRERDRFVGFVLESVETIPAADKIQGYARFIDDNTLQVDDHTQLTSQKIIAQRIVIATGSRPSWPASWNEL
GDRLIINDDVFNWDDLPESVAVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQADRLTMQTNVPHIFIAGDASNQ
LPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDPQIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLK
NKGILRIYGEQGTGRFLGAEMMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
CLRCPGE

Sequences:

>Translated_487_residues
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEAVHQIEKAPGFGIHPQGKPLI
NGREVMDRVKRERDRFVGFVLESVETIPAADKIQGYARFIDDNTLQVDDHTQLTSQKIIAQRIVIATGSRPSWPASWNEL
GDRLIINDDVFNWDDLPESVAVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQADRLTMQTNVPHIFIAGDASNQ
LPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDPQIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLK
NKGILRIYGEQGTGRFLGAEMMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
CLRCPGE
>Mature_487_residues
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEAVHQIEKAPGFGIHPQGKPLI
NGREVMDRVKRERDRFVGFVLESVETIPAADKIQGYARFIDDNTLQVDDHTQLTSQKIIAQRIVIATGSRPSWPASWNEL
GDRLIINDDVFNWDDLPESVAVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQADRLTMQTNVPHIFIAGDASNQ
LPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDPQIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLK
NKGILRIYGEQGTGRFLGAEMMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
CLRCPGE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=478, Percent_Identity=27.4058577405858, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI50301238, Length=468, Percent_Identity=25.4273504273504, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI148277065, Length=460, Percent_Identity=22.8260869565217, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI33519430, Length=460, Percent_Identity=22.8260869565217, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI33519428, Length=460, Percent_Identity=22.8260869565217, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI33519426, Length=460, Percent_Identity=22.8260869565217, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI148277071, Length=466, Percent_Identity=23.175965665236, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786307, Length=469, Percent_Identity=24.9466950959488, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=27.7310924369748, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789915, Length=371, Percent_Identity=29.3800539083558, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=24.5202558635394, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32565766, Length=477, Percent_Identity=27.4633123689727, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17557007, Length=305, Percent_Identity=25.5737704918033, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71983429, Length=370, Percent_Identity=24.8648648648649, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983419, Length=370, Percent_Identity=24.8648648648649, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=27.2151898734177, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325166, Length=395, Percent_Identity=28.1012658227848, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6325240, Length=349, Percent_Identity=25.7879656160458, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21358499, Length=477, Percent_Identity=27.4633123689727, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640549, Length=494, Percent_Identity=26.7206477732794, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24640553, Length=494, Percent_Identity=26.7206477732794, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=26.4887063655031, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17737741, Length=493, Percent_Identity=23.9350912778905, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 53182; Mature: 53182

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEA
CCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPGFGIHPQGKPLINGREVMDRVKRERDRFVGFVLESVETIPAADKIQGYARFI
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DDNTLQVDDHTQLTSQKIIAQRIVIATGSRPSWPASWNELGDRLIINDDVFNWDDLPESV
CCCEEEECCCHHHHHHHHHHHEEEEEECCCCCCCCCHHHHCCEEEEECCCCCCCCCCCHH
AVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
HHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEE
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQAD
EEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEECCCCCHHH
RLTMQTNVPHIFIAGDASNQLPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDP
CEEEECCCCEEEEECCCCCCCCEEECCCCCCEEEECCCCCCHHHCCCCCCCCEEEEECCC
QIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLKNKGILRIYGEQGTGRFLGAE
CEEEEHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEHH
MMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
CLRCPGE
HHCCCCC
>Mature Secondary Structure
MKTLNVDVAVIGGGTAGLGAYRAAKLLTPNVVMIEGGEYGTTCARVGCMPSKLLIAAAEA
CCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPGFGIHPQGKPLINGREVMDRVKRERDRFVGFVLESVETIPAADKIQGYARFI
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DDNTLQVDDHTQLTSQKIIAQRIVIATGSRPSWPASWNELGDRLIINDDVFNWDDLPESV
CCCEEEECCCHHHHHHHHHHHEEEEEECCCCCCCCCHHHHCCEEEEECCCCCCCCCCCHH
AVFGPGVIGLELGQALHRLGVQVKMFGVGGAVGPLTDSIVRDYAAKTLGDEFYLDPDVKV
HHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEE
ELMQREGDKVFIRYLDKSGRPQEIMVDYVLAATGRRPNVDKLGLENTSLILDERGVPQAD
EEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEECCCCCHHH
RLTMQTNVPHIFIAGDASNQLPLLHEASDQARIAGVNAGGFPEVVPGLRRSPISVVFSDP
CEEEECCCCEEEEECCCCCCCCEEECCCCCCEEEECCCCCCHHHCCCCCCCCEEEEECCC
QIAMVGATFRELAQKFSACGCFEIGEVSFENQGRSRVMLKNKGILRIYGEQGTGRFLGAE
CEEEEHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEHH
MMGPSAEHIAHLLAWAHQQQMTIDQMLDMPFYHPVIEEGLRTALRDLQSKLKLGADEAER
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
CLRCPGE
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]