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The map label for this gene is thiC

Identifier: 218930744

GI number: 218930744

Start: 4190602

End: 4192647

Strand: Direct

Name: thiC

Synonym: YPO3739

Alternate gene names: 218930744

Gene position: 4190602-4192647 (Clockwise)

Preceding gene: 218930743

Following gene: 218930745

Centisome position: 90.05

GC content: 51.81

Gene sequence:

>2046_bases
ATGTCTAATAATACAACGTCATTACCCGCTGAAAATTCGTCACACCCACGTAAAGGCACACCTATTCGTAAAAAGCAGCG
CGAAGAGGCCCAACAGTTTATTAATACCTTACAAGGCGTTACTTTTCCCAACTCCCAACGTATTTATCTACAAGGCTCTC
GGCCCGATATTCAAGTGCCGATGCGTGAAATCCAACTCAGCCCGACGCAAATCGGCGGCAGTAAAAACGAACCACGCTAT
GAAGATAATGAGGCCATCCCGGTCTATGACACCTCCGGTCCCTATGGTGACCCACAAGCTAAACTGGATGTTCATAACGG
GCTGCCTAAACTGCGTGCCGCTTGGGTCGCAGATCGCCAAGATACTGAAGCGCTGGCATCTGTCAGTTCCGGCTTTACCC
AACAGCGTTTGGCTGATGAAGGCCTGGACCATTTACGTTTTGAGCATCTACCCCGCCCACGGAAAGCAGCCACTGGTCAA
TGTGTGACTCAGTTGCATTACGCCCGACAGGGGAAAATCACGCCAGAGATGGAGTTTATCGCCCTACGGGAAAATATGGG
CCGTGAACGTATTCGGGGTGAAGTCTTGCTTCAACAACATCCGGGACAAGCGTTTGGTGCCCATCTGCCGGAAAATATCA
CCGCCGAGTTTGTGCGTCAGGAAGTGGCGGCCGGCCGAGCCATCATCCCCGCCAATATTAATCACCCAGAATCTGAACCA
ATGATTATTGGCCGTAATTTTCTGGTCAAAGTGAATGCCAACATCGGTAATTCCGCCGTGACCTCTTCCATTGAAGAAGA
GGTAGAAAAACTGGTCTGGTCTACCCGTTGGGGTGCCGATACGGTGATGGACTTATCTACGGGCCGCTATATTCATGAAA
CGCGGGAATGGATCCTACGTAACAGCCCGGTCCCTATTGGCACGGTACCTATCTATCAGGCGCTGGAAAAAGTTAATGGC
GTGGCCGAAAATCTGACCTGGGAAATGTTCCGTGACACCCTGTTAGAGCAGGCAGAGCAAGGGGTAGACTATTTTACTCT
CCACGCGGGGGTCTTGTTGCGCTATGTGCCGATGACTGCCAAACGCCTAACCGGTATCGTCTCTCGCGGCGGTTCAATTA
TGGCAAAATGGTGCCTTTCGCATCATCAGGAAAACTTCCTGTATCAGCATTTCCGCGAAATCTGTCAGATTTGTGCAGCC
TATGACGTTTCATTATCACTGGGCGATGGCCTGCGCCCTGGCTCTATTCAAGATGCCAATGATGAGGCCCAATTCGCCGA
ACTGCATACCTTGGGTGAATTGACCAAAATCGCCTGGGAGTATGATGTACAGGTGATGATCGAAGGCCCAGGGCATGTGC
CGATGCAGATGATCCGCCGCAATATGACCGAGGAACTGGAACACTGCCACGAAGCGCCATTTTATACCTTAGGCCCACTG
ACCACGGACATCGCACCGGGCTATGACCACTTTACCTCAGGGATTGGTGCAGCGATGATCGGCTGGTTCGGTTGCGCCAT
GCTCTGTTATGTCACCCCCAAAGAGCACCTTGGTCTGCCGAATAAAGAGGATGTCAAACAGGGGCTTATTACTTACAAAA
TTGCCGCGCACGCCGCAGATTTGGCTAAAGGCCACCCCGGTGCGCAAATTCGTGATAACGCCATGTCCAAAGCTCGCTTT
GAGTTCCGCTGGGAAGATCAATTCAATTTGGCGCTTGATCCAGCAACAGCCCGCGCTTACCACGATGAAACCTTGCCGCA
AGAGTCCGGGAAAGTCGCTCATTTTTGTTCCATGTGCGGCCCGAAATTCTGTTCAATGAAAATCTCACAAGAGGTCCGCG
ATTATGCTGCGGCACAAGAACAAGCGGCCGCACAAGCACAAGCCGCTACACCAACCACCGCAGCACAACCAATAGACATC
ACGCAGCCAATTAATATGCTGCAATCAGGGATGGAAAAAATGTCGGCCGAGTTTCGCTCCCGTGGCAGTGAGCTATACCA
CCGCCCGGCGAATCTGAGTGCGGAGGCCAATAATGAGCCAACTTGA

Upstream 100 bases:

>100_bases
ACCGGTCACTGGCCAACATCGTTACTCCCGCCAAGCTCCAGACAAGCAATTTTCCCAATACACCACACTGGCTCTATAAA
CCACACTGGTAGGAATTGCT

Downstream 100 bases:

>100_bases
TGCCCTCTCTCCTGTGGCTACACCAGGTTTCCCCTCCACGGAACAGCGTCTTGGGCTCTACCCAGTGGTGGACTCATTAT
TATGGATAGAACGCCTGCTG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MSNNTTSLPAENSSHPRKGTPIRKKQREEAQQFINTLQGVTFPNSQRIYLQGSRPDIQVPMREIQLSPTQIGGSKNEPRY
EDNEAIPVYDTSGPYGDPQAKLDVHNGLPKLRAAWVADRQDTEALASVSSGFTQQRLADEGLDHLRFEHLPRPRKAATGQ
CVTQLHYARQGKITPEMEFIALRENMGRERIRGEVLLQQHPGQAFGAHLPENITAEFVRQEVAAGRAIIPANINHPESEP
MIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNG
VAENLTWEMFRDTLLEQAEQGVDYFTLHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICQICAA
YDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPL
TTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARF
EFRWEDQFNLALDPATARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQEQAAAQAQAATPTTAAQPIDI
TQPINMLQSGMEKMSAEFRSRGSELYHRPANLSAEANNEPT

Sequences:

>Translated_681_residues
MSNNTTSLPAENSSHPRKGTPIRKKQREEAQQFINTLQGVTFPNSQRIYLQGSRPDIQVPMREIQLSPTQIGGSKNEPRY
EDNEAIPVYDTSGPYGDPQAKLDVHNGLPKLRAAWVADRQDTEALASVSSGFTQQRLADEGLDHLRFEHLPRPRKAATGQ
CVTQLHYARQGKITPEMEFIALRENMGRERIRGEVLLQQHPGQAFGAHLPENITAEFVRQEVAAGRAIIPANINHPESEP
MIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNG
VAENLTWEMFRDTLLEQAEQGVDYFTLHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICQICAA
YDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPL
TTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARF
EFRWEDQFNLALDPATARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQEQAAAQAQAATPTTAAQPIDI
TQPINMLQSGMEKMSAEFRSRGSELYHRPANLSAEANNEPT
>Mature_680_residues
SNNTTSLPAENSSHPRKGTPIRKKQREEAQQFINTLQGVTFPNSQRIYLQGSRPDIQVPMREIQLSPTQIGGSKNEPRYE
DNEAIPVYDTSGPYGDPQAKLDVHNGLPKLRAAWVADRQDTEALASVSSGFTQQRLADEGLDHLRFEHLPRPRKAATGQC
VTQLHYARQGKITPEMEFIALRENMGRERIRGEVLLQQHPGQAFGAHLPENITAEFVRQEVAAGRAIIPANINHPESEPM
IIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGV
AENLTWEMFRDTLLEQAEQGVDYFTLHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICQICAAY
DVSLSLGDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLT
TDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFE
FRWEDQFNLALDPATARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQEQAAAQAQAATPTTAAQPIDIT
QPINMLQSGMEKMSAEFRSRGSELYHRPANLSAEANNEPT

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=646, Percent_Identity=83.5913312693499, Blast_Score=1129, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_YERPE (Q8ZAQ2)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AC0455
- RefSeq:   NP_667829.1
- RefSeq:   NP_994395.1
- RefSeq:   YP_002348619.1
- IntAct:   Q8ZAQ2
- GeneID:   1145438
- GeneID:   1176565
- GeneID:   2766733
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3739
- KEGG:   ypk:y0491
- KEGG:   ypm:YP_3102
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   YPES187410:Y0491-MONOMER
- BioCyc:   YPES214092:YPO3739-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 75879; Mature: 75747

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: BINDING 254-254 BINDING 283-283 BINDING 312-312 BINDING 348-348 BINDING 448-448 BINDING 475-475

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNTTSLPAENSSHPRKGTPIRKKQREEAQQFINTLQGVTFPNSQRIYLQGSRPDIQVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCC
MREIQLSPTQIGGSKNEPRYEDNEAIPVYDTSGPYGDPQAKLDVHNGLPKLRAAWVADRQ
HHHHCCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHCCCHHHHHHHHCCCH
DTEALASVSSGFTQQRLADEGLDHLRFEHLPRPRKAATGQCVTQLHYARQGKITPEMEFI
HHHHHHHHHCCHHHHHHHHHCHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCCCCHHHH
ALRENMGRERIRGEVLLQQHPGQAFGAHLPENITAEFVRQEVAAGRAIIPANINHPESEP
HHHHHCCHHHHHHHHHEECCCCCHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCC
MIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILR
EEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHC
NSPVPIGTVPIYQALEKVNGVAENLTWEMFRDTLLEQAEQGVDYFTLHAGVLLRYVPMTA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
KRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICQICAAYDVSLSLGDGLRPGSIQDAN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCC
DEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPL
CHHHHHHHHHHHHHHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCC
TTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAAD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHH
LAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPATARAYHDETLPQESGKVAHFCSMCG
HHCCCCCCCHHHHHHHHCEEEEEECCCCCEEECCHHHHHHHHHCCCCCCCCHHHHHHHCC
PKFCSMKISQEVRDYAAAQEQAAAQAQAATPTTAAQPIDITQPINMLQSGMEKMSAEFRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RGSELYHRPANLSAEANNEPT
CCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNNTTSLPAENSSHPRKGTPIRKKQREEAQQFINTLQGVTFPNSQRIYLQGSRPDIQVP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCC
MREIQLSPTQIGGSKNEPRYEDNEAIPVYDTSGPYGDPQAKLDVHNGLPKLRAAWVADRQ
HHHHCCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHCCCHHHHHHHHCCCH
DTEALASVSSGFTQQRLADEGLDHLRFEHLPRPRKAATGQCVTQLHYARQGKITPEMEFI
HHHHHHHHHCCHHHHHHHHHCHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCCCCHHHH
ALRENMGRERIRGEVLLQQHPGQAFGAHLPENITAEFVRQEVAAGRAIIPANINHPESEP
HHHHHCCHHHHHHHHHEECCCCCHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCC
MIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILR
EEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHC
NSPVPIGTVPIYQALEKVNGVAENLTWEMFRDTLLEQAEQGVDYFTLHAGVLLRYVPMTA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
KRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICQICAAYDVSLSLGDGLRPGSIQDAN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCC
DEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPL
CHHHHHHHHHHHHHHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCC
TTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAAD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHH
LAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPATARAYHDETLPQESGKVAHFCSMCG
HHCCCCCCCHHHHHHHHCEEEEEECCCCCEEECCHHHHHHHHHCCCCCCCCHHHHHHHCC
PKFCSMKISQEVRDYAAAQEQAAAQAQAATPTTAAQPIDITQPINMLQSGMEKMSAEFRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RGSELYHRPANLSAEANNEPT
CCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430