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The map label for this gene is dacB [H]

Identifier: 218930520

GI number: 218930520

Start: 3916101

End: 3917549

Strand: Direct

Name: dacB [H]

Synonym: YPO3506

Alternate gene names: 218930520

Gene position: 3916101-3917549 (Clockwise)

Preceding gene: 218930518

Following gene: 218930521

Centisome position: 84.15

GC content: 48.24

Gene sequence:

>1449_bases
ATGCATTTTTCACGAATTGTCAGTGGATTGGCCTGTTCTATCATACTCAATATCAGTGTATCTAATGCCAATGCGGCTCA
GGTCGAGAATTACACTCAGTATCTGCCTGATGGGGCAAATCTTGCGTTAATGGTCCAAAAAATTGGGGCATCAACCCCTG
CAATAGACTATCACGCTCAGCAGATGGCGCTACCGGCCAGTACGCAAAAAGTACTGACGGCCTTGGCAGCGTTATTGCAA
CTCGGCCCAGACTTTCGCTTCAATACTACCTTGGAAAGCCATGGCACGATTACTGATGGTGTGTTGCGAGGTAATTTAAT
TGCCCGGTTTGGTGGTGACCCAACGTTAACGCGTCAACAACTGCGTAATATGGTTGCGACTCTCAGAAAAGCGGGGGTTA
AACAGATCGCGGGGGATGTGGTGATTGATACCTCAGTATTCGCCAGTCATGACAAAGCACCGGGTTGGCCATGGAACGAT
ATGACGCAATGCTTTAGTGCGCCGCCTGCTGCTGCCATTGTTGATCGCAACTGTTTTTCAGTCTCTCTTTACAGTGCGCC
CAATCCCGGCGATACCGCATTTATCCGTGTCGCGTCTTATTATCCGGTGCAAATGTTCAGTGAAGTCCGCACGTTAGCCA
AAGGCTCACCCGATGCACAATATTGTGAGTTGGACGTCGTGCCAGGCGAATTAAACCGGTTTACGCTTACCGGTTGTCTG
ACACAGCGCAGCGAACCTTTGCCATTGGCCTTTGCTGTGCAAAATGGGGCCAGCTACGCCGGGGCTATCTTAAAAGATGA
ATTGAAAAAAGCTGATATTCAAATTGATGGCAATCTGCGCCGCCAAACAATACCGAGTCCTGCGGGAAACGTGCTAGCAC
AGACCCAATCAGCCCCCTTACATGATTTGCTGAAAATCATGCTGAAAAAATCAGACAATATGATCGCTGACACCGTCTTC
AGAACCATTGGTCATCAGCGTTTTGGGGTTCCGGGTACTTGGCGCTCGGGTGCCGATGCCGTTCGCCAAATTTTACGCCA
AAAAGCAGGCGTTGATCTTGGTAACAGCATTGTGGTGGATGGATCTGGCCTATCGCGCCATAATCTTATTTCACCAGCAA
CAATGATGCAGGTTTTGCAATATATTGCTCAAAATGATCAAGAGCTTAATTTTATATCAATGCTGCCTCTAGCAGGATAT
GACGGTACTCTACGTTATCGTGGTGGCCTGCATGAAGCGGGGGTCAACGGTAAAGTTTCAGCGAAGACGGGTGCACTGCA
AGGGGTTTACAATCTTGCCGGTTTCATTACTACCGCTAGTGGGCAACGTATGGCATTTGTACAATTTTTATCCGGCTATG
CCGTTCCACCTGAAGACCAAAAAAATCGCCGAGCCCCCTTGGTTCGATTTGAGAGCCGCCTCTATAAAGATATTTATCAG
AATAACTGA

Upstream 100 bases:

>100_bases
ATATGCATTTTAACGTAGAGTTCCTTTAGGGTCATCGTTTACTTTCTCCCTCGTTGCACCGTAGTATGACAACACATTAT
TCAGCAGTTATACCGAAATT

Downstream 100 bases:

>100_bases
GAGCTATGAAATTACTGATTGTTGAAGATGATGAACTGCTACAACGCGGTATAGCCATGGCGCTGACCAGTGAAGGCTAT
GTCTGCGACTGCGCAGCTAC

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MHFSRIVSGLACSIILNISVSNANAAQVENYTQYLPDGANLALMVQKIGASTPAIDYHAQQMALPASTQKVLTALAALLQ
LGPDFRFNTTLESHGTITDGVLRGNLIARFGGDPTLTRQQLRNMVATLRKAGVKQIAGDVVIDTSVFASHDKAPGWPWND
MTQCFSAPPAAAIVDRNCFSVSLYSAPNPGDTAFIRVASYYPVQMFSEVRTLAKGSPDAQYCELDVVPGELNRFTLTGCL
TQRSEPLPLAFAVQNGASYAGAILKDELKKADIQIDGNLRRQTIPSPAGNVLAQTQSAPLHDLLKIMLKKSDNMIADTVF
RTIGHQRFGVPGTWRSGADAVRQILRQKAGVDLGNSIVVDGSGLSRHNLISPATMMQVLQYIAQNDQELNFISMLPLAGY
DGTLRYRGGLHEAGVNGKVSAKTGALQGVYNLAGFITTASGQRMAFVQFLSGYAVPPEDQKNRRAPLVRFESRLYKDIYQ
NN

Sequences:

>Translated_482_residues
MHFSRIVSGLACSIILNISVSNANAAQVENYTQYLPDGANLALMVQKIGASTPAIDYHAQQMALPASTQKVLTALAALLQ
LGPDFRFNTTLESHGTITDGVLRGNLIARFGGDPTLTRQQLRNMVATLRKAGVKQIAGDVVIDTSVFASHDKAPGWPWND
MTQCFSAPPAAAIVDRNCFSVSLYSAPNPGDTAFIRVASYYPVQMFSEVRTLAKGSPDAQYCELDVVPGELNRFTLTGCL
TQRSEPLPLAFAVQNGASYAGAILKDELKKADIQIDGNLRRQTIPSPAGNVLAQTQSAPLHDLLKIMLKKSDNMIADTVF
RTIGHQRFGVPGTWRSGADAVRQILRQKAGVDLGNSIVVDGSGLSRHNLISPATMMQVLQYIAQNDQELNFISMLPLAGY
DGTLRYRGGLHEAGVNGKVSAKTGALQGVYNLAGFITTASGQRMAFVQFLSGYAVPPEDQKNRRAPLVRFESRLYKDIYQ
NN
>Mature_482_residues
MHFSRIVSGLACSIILNISVSNANAAQVENYTQYLPDGANLALMVQKIGASTPAIDYHAQQMALPASTQKVLTALAALLQ
LGPDFRFNTTLESHGTITDGVLRGNLIARFGGDPTLTRQQLRNMVATLRKAGVKQIAGDVVIDTSVFASHDKAPGWPWND
MTQCFSAPPAAAIVDRNCFSVSLYSAPNPGDTAFIRVASYYPVQMFSEVRTLAKGSPDAQYCELDVVPGELNRFTLTGCL
TQRSEPLPLAFAVQNGASYAGAILKDELKKADIQIDGNLRRQTIPSPAGNVLAQTQSAPLHDLLKIMLKKSDNMIADTVF
RTIGHQRFGVPGTWRSGADAVRQILRQKAGVDLGNSIVVDGSGLSRHNLISPATMMQVLQYIAQNDQELNFISMLPLAGY
DGTLRYRGGLHEAGVNGKVSAKTGALQGVYNLAGFITTASGQRMAFVQFLSGYAVPPEDQKNRRAPLVRFESRLYKDIYQ
NN

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=482, Percent_Identity=79.045643153527, Blast_Score=798, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52177; Mature: 52177

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFSRIVSGLACSIILNISVSNANAAQVENYTQYLPDGANLALMVQKIGASTPAIDYHAQ
CCHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCHHHH
QMALPASTQKVLTALAALLQLGPDFRFNTTLESHGTITDGVLRGNLIARFGGDPTLTRQQ
HHCCCCHHHHHHHHHHHHHHCCCCCEEECEECCCCCEECCCCCCCEEEEECCCCCHHHHH
LRNMVATLRKAGVKQIAGDVVIDTSVFASHDKAPGWPWNDMTQCFSAPPAAAIVDRNCFS
HHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCCCCHHHHHHHCCCCCHHEECCCCEE
VSLYSAPNPGDTAFIRVASYYPVQMFSEVRTLAKGSPDAQYCELDVVPGELNRFTLTGCL
EEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEH
TQRSEPLPLAFAVQNGASYAGAILKDELKKADIQIDGNLRRQTIPSPAGNVLAQTQSAPL
HCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHCCCCCCH
HDLLKIMLKKSDNMIADTVFRTIGHQRFGVPGTWRSGADAVRQILRQKAGVDLGNSIVVD
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEE
GSGLSRHNLISPATMMQVLQYIAQNDQELNFISMLPLAGYDGTLRYRGGLHEAGVNGKVS
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECEECCCCCCEEECCCCCCCCCCCEEE
AKTGALQGVYNLAGFITTASGQRMAFVQFLSGYAVPPEDQKNRRAPLVRFESRLYKDIYQ
CCHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
NN
CC
>Mature Secondary Structure
MHFSRIVSGLACSIILNISVSNANAAQVENYTQYLPDGANLALMVQKIGASTPAIDYHAQ
CCHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCHHHH
QMALPASTQKVLTALAALLQLGPDFRFNTTLESHGTITDGVLRGNLIARFGGDPTLTRQQ
HHCCCCHHHHHHHHHHHHHHCCCCCEEECEECCCCCEECCCCCCCEEEEECCCCCHHHHH
LRNMVATLRKAGVKQIAGDVVIDTSVFASHDKAPGWPWNDMTQCFSAPPAAAIVDRNCFS
HHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCCCCCCHHHHHHHCCCCCHHEECCCCEE
VSLYSAPNPGDTAFIRVASYYPVQMFSEVRTLAKGSPDAQYCELDVVPGELNRFTLTGCL
EEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEH
TQRSEPLPLAFAVQNGASYAGAILKDELKKADIQIDGNLRRQTIPSPAGNVLAQTQSAPL
HCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHCCCCCCH
HDLLKIMLKKSDNMIADTVFRTIGHQRFGVPGTWRSGADAVRQILRQKAGVDLGNSIVVD
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEE
GSGLSRHNLISPATMMQVLQYIAQNDQELNFISMLPLAGYDGTLRYRGGLHEAGVNGKVS
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECEECCCCCCEEECCCCCCCCCCCEEE
AKTGALQGVYNLAGFITTASGQRMAFVQFLSGYAVPPEDQKNRRAPLVRFESRLYKDIYQ
CCHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
NN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]