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The map label for this gene is emrB [H]

Identifier: 218930293

GI number: 218930293

Start: 3639538

End: 3641073

Strand: Direct

Name: emrB [H]

Synonym: YPO3268

Alternate gene names: 218930293

Gene position: 3639538-3641073 (Clockwise)

Preceding gene: 218930292

Following gene: 218930295

Centisome position: 78.21

GC content: 53.84

Gene sequence:

>1536_bases
GTGGCACAAAAACCGCTTGAAGGAGCACCACTCGCCTGGATGACGGTGGCGCTTTCATTAGCGACCTTCATGCAGGTGCT
GGACTCCACCATTGCTAACGTGGCCATCCCAACGATTGCGGGTGATTTAGGCTCGTCCAACTCGCAAGGGACGTGGGTAA
TCACCTCATTCGGGGTGGCGAATGCAATTTCGATCCCCGTCACCGGCTGGCTAGCCAAGCGAGTCGGTGAAGTGCGGCTG
TTCTTGTGGTCAACAGGCTTGTTTGTGCTGGCCTCATGGCTATGCGGCATGTCAAACAGCTTGGGGATGCTTATCTTCTT
CCGCGTTATCCAGGGTCTGGTCGCTGGGCCATTGATCCCACTCTCGCAAAGCCTGCTACTCAACAACTATCCGCCGGCGA
AGCGAAGTATGGCCCTGGCGTTGTGGTCGATGACGATTGTTGTCGCCCCGATATTTGGGCCGATCCTCGGCGGTTATATC
AGTGATAACTATCACTGGGGCTGGATCTTCTTTATCAATATCCCTATCGGTCTGGTGGTGGTCCTGCTGGCGGGCAGTAC
CCTGAAAGGACGAGAGACCAAAACAGAGATCAGACCCATTGATACCATCGGGCTGGTGTTATTGGTGGTCGGTATTGGTG
CCTTGCAAATCATGCTTGATCAAGGCAAGGAGCTGGATTGGTTTAACTCGACAGAAATTATCGTCCTGACCGTGGTGGCT
GTCGTGGCTATTACCTTCCTGATTGTCTGGGAGTTAACCGACGATCATCCGGTGATTGATTTGTCGCTGTTTAAATCGCG
CAACTTTACCATTGGCTGTTTGTGTCTCAGCCTGGCCTATATGTTGTACTTTGGTGCGATTGTCTTACTGCCCCAGCTAT
TGCAGGAGGTGTATGGCTATACCGCCACCTGGGCCGGATTGGCATCGGCACCGGTGGGTATACTGCCCGTGCTGCTGTCG
CCATTAATTGGTCGTTTTGCCCATCGAATTGATATGCGCCAGTTAGTGACATTTAGCTTCATTATGTATGCGGTGTGTTT
CTACTGGCGAGCATATACCTTCGAACCGGGGATGGATTTTGGTGCCTCAGCATGGCCGCAGTTCTTCCAAGGCTTCGCCA
TTGCCTGCTTCTTCATGCCATTGACCACGATTACGTTATCCGGTTTGCCACCGGAGCGAATGGCCGCGGCATCCAGTTTG
TCTAACTTTATGCGAACACTGGCAGGGTCGATTGGTACCTCAATCACTACCACCCTCTGGACGCAAAGAGAGTCGTTGCA
CCACGCGCAGTTGACGGAGTTTGTTAACCCGTACAACCCGAACGCGACGCAGGCCTACCAGGAGTTGGAAAAACTGGGGA
TGAGCCAGCAACAGGCTTCGGCCTACCTGGCCCGTGAAATTACCAATCAGGGGCTGATTATCTCTGCGAATGAGATTTTC
TGGCTCTCGGCGGGGGTGTTCCTAATGCTGCTGGCCCTGATCTGGTTTGCCCGCCCTCCTTTCACCTCCGGTGGTGGCGG
CGGCGGTGCGCATTAA

Upstream 100 bases:

>100_bases
CATTAGATTTCGCGCCAGTTAATCAAATAATTAGCGATATTATTCATGCAAACGCCGATTAATATCATGCCAATGCGGGT
TACGTGCACCGGAGGCTATT

Downstream 100 bases:

>100_bases
CCTGCATTAACCTGCAATTTTTAGCCAAATAGCGGTCAACAAAAAAGGGGGCCCTGAACTCATCAGCGCCCCCTTTTATT
TTTACCGTTCTAAAACGTCT

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MAQKPLEGAPLAWMTVALSLATFMQVLDSTIANVAIPTIAGDLGSSNSQGTWVITSFGVANAISIPVTGWLAKRVGEVRL
FLWSTGLFVLASWLCGMSNSLGMLIFFRVIQGLVAGPLIPLSQSLLLNNYPPAKRSMALALWSMTIVVAPIFGPILGGYI
SDNYHWGWIFFINIPIGLVVVLLAGSTLKGRETKTEIRPIDTIGLVLLVVGIGALQIMLDQGKELDWFNSTEIIVLTVVA
VVAITFLIVWELTDDHPVIDLSLFKSRNFTIGCLCLSLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGILPVLLS
PLIGRFAHRIDMRQLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFFQGFAIACFFMPLTTITLSGLPPERMAAASSL
SNFMRTLAGSIGTSITTTLWTQRESLHHAQLTEFVNPYNPNATQAYQELEKLGMSQQQASAYLAREITNQGLIISANEIF
WLSAGVFLMLLALIWFARPPFTSGGGGGGAH

Sequences:

>Translated_511_residues
MAQKPLEGAPLAWMTVALSLATFMQVLDSTIANVAIPTIAGDLGSSNSQGTWVITSFGVANAISIPVTGWLAKRVGEVRL
FLWSTGLFVLASWLCGMSNSLGMLIFFRVIQGLVAGPLIPLSQSLLLNNYPPAKRSMALALWSMTIVVAPIFGPILGGYI
SDNYHWGWIFFINIPIGLVVVLLAGSTLKGRETKTEIRPIDTIGLVLLVVGIGALQIMLDQGKELDWFNSTEIIVLTVVA
VVAITFLIVWELTDDHPVIDLSLFKSRNFTIGCLCLSLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGILPVLLS
PLIGRFAHRIDMRQLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFFQGFAIACFFMPLTTITLSGLPPERMAAASSL
SNFMRTLAGSIGTSITTTLWTQRESLHHAQLTEFVNPYNPNATQAYQELEKLGMSQQQASAYLAREITNQGLIISANEIF
WLSAGVFLMLLALIWFARPPFTSGGGGGGAH
>Mature_510_residues
AQKPLEGAPLAWMTVALSLATFMQVLDSTIANVAIPTIAGDLGSSNSQGTWVITSFGVANAISIPVTGWLAKRVGEVRLF
LWSTGLFVLASWLCGMSNSLGMLIFFRVIQGLVAGPLIPLSQSLLLNNYPPAKRSMALALWSMTIVVAPIFGPILGGYIS
DNYHWGWIFFINIPIGLVVVLLAGSTLKGRETKTEIRPIDTIGLVLLVVGIGALQIMLDQGKELDWFNSTEIIVLTVVAV
VAITFLIVWELTDDHPVIDLSLFKSRNFTIGCLCLSLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGILPVLLSP
LIGRFAHRIDMRQLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFFQGFAIACFFMPLTTITLSGLPPERMAAASSLS
NFMRTLAGSIGTSITTTLWTQRESLHHAQLTEFVNPYNPNATQAYQELEKLGMSQQQASAYLAREITNQGLIISANEIFW
LSAGVFLMLLALIWFARPPFTSGGGGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=499, Percent_Identity=84.1683366733467, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=503, Percent_Identity=62.2266401590457, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1790195, Length=439, Percent_Identity=31.8906605922551, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87081983, Length=431, Percent_Identity=27.6102088167053, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=28.960396039604, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322958, Length=531, Percent_Identity=21.8455743879473, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=29.3333333333333, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=314, Percent_Identity=22.2929936305732, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55705; Mature: 55574

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKPLEGAPLAWMTVALSLATFMQVLDSTIANVAIPTIAGDLGSSNSQGTWVITSFGVA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
NAISIPVTGWLAKRVGEVRLFLWSTGLFVLASWLCGMSNSLGMLIFFRVIQGLVAGPLIP
CEEECCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
LSQSLLLNNYPPAKRSMALALWSMTIVVAPIFGPILGGYISDNYHWGWIFFINIPIGLVV
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEEEEEECHHHHHH
VLLAGSTLKGRETKTEIRPIDTIGLVLLVVGIGALQIMLDQGKELDWFNSTEIIVLTVVA
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
VVAITFLIVWELTDDHPVIDLSLFKSRNFTIGCLCLSLAYMLYFGAIVLLPQLLQEVYGY
HHHHHHHHHEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TATWAGLASAPVGILPVLLSPLIGRFAHRIDMRQLVTFSFIMYAVCFYWRAYTFEPGMDF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
GASAWPQFFQGFAIACFFMPLTTITLSGLPPERMAAASSLSNFMRTLAGSIGTSITTTLW
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TQRESLHHAQLTEFVNPYNPNATQAYQELEKLGMSQQQASAYLAREITNQGLIISANEIF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCEE
WLSAGVFLMLLALIWFARPPFTSGGGGGGAH
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQKPLEGAPLAWMTVALSLATFMQVLDSTIANVAIPTIAGDLGSSNSQGTWVITSFGVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
NAISIPVTGWLAKRVGEVRLFLWSTGLFVLASWLCGMSNSLGMLIFFRVIQGLVAGPLIP
CEEECCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
LSQSLLLNNYPPAKRSMALALWSMTIVVAPIFGPILGGYISDNYHWGWIFFINIPIGLVV
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEEEEEECHHHHHH
VLLAGSTLKGRETKTEIRPIDTIGLVLLVVGIGALQIMLDQGKELDWFNSTEIIVLTVVA
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
VVAITFLIVWELTDDHPVIDLSLFKSRNFTIGCLCLSLAYMLYFGAIVLLPQLLQEVYGY
HHHHHHHHHEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TATWAGLASAPVGILPVLLSPLIGRFAHRIDMRQLVTFSFIMYAVCFYWRAYTFEPGMDF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
GASAWPQFFQGFAIACFFMPLTTITLSGLPPERMAAASSLSNFMRTLAGSIGTSITTTLW
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TQRESLHHAQLTEFVNPYNPNATQAYQELEKLGMSQQQASAYLAREITNQGLIISANEIF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCEE
WLSAGVFLMLLALIWFARPPFTSGGGGGGAH
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]