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The map label for this gene is cru [H]

Identifier: 218930032

GI number: 218930032

Start: 3331058

End: 3332242

Strand: Direct

Name: cru [H]

Synonym: YPO2983

Alternate gene names: 218930032

Gene position: 3331058-3332242 (Clockwise)

Preceding gene: 218930030

Following gene: 218930033

Centisome position: 71.58

GC content: 44.56

Gene sequence:

>1185_bases
ATGTCCCATATTCTACAGTTTGCGTTAGCCCTCGTTGTGGTAGCTATACTGGCTTTGGCAATCTGTAAAGATCGTAAAAG
CATCCGTATTCGATTTGTTATTCAATTGTTGGTTATAGAAGTGTTGCTGGCATACTTCTTCCTCCATTCGGAAGCTGGAC
TGGGTTTTGTGAAAGGATTCGCAGGGTTTTTCGACACATTGCTAGGATTTGCAGCCGCCGGTACCGATTTTGTCTTTAAT
GATATGGGTGATAAAGGTCTCGCGTTCTTCTTCCTAAGAGTGTTGTGTCCTATTGTCTTTATTTCTGCATTAATCGGTAT
TTTGCAATACATCAAAGTTTTGCCGATCGTCATTCGTCTGATCGGTACATTACTGTCCAAAGTTAACGGTATGGGCAAAC
TGGAATCATTCAACGCCGTCAGTTCACTGATTCTCGGACAATCTGAAAACTTTATTGCCTATAAAGATATTTTGGGCAAG
ATGTCTGAAAAACGCATGTACACCATGGCGGCAACAGCAATGTCGACCGTTTCCATGTCGATTGTTGGCGCTTATATGTC
CATGCTGGATGCGAAATTTGTGGTCGCCGCCTTGGTACTCAACATGTTCAGTACCTTTATCGTGTTGTCATTGATCAACC
CGTACAAAGTGGGCGAAGAACAAGATTTGCAACTGAGTAATCTGCATGAAGGCCAAAGCTTCTTTGAAATGCTGGGAGAA
TATATTCTGGCTGGTTTCAAAGTAGCCATTATCGTTGCTGCAATGTTGATTGGTTTCATCGCGTTGATTGCTGCGGTGAA
TGCTCTATTCACCTTACTGTTCGGTATTAGCTTCCAAGGAATTTTGGGTTATGTGTTCTTCCCGTTTGCTTGGGTGATGG
GTATCCCTGCCCATGAGGCCTTACAGGTCGGGAGTATCATGGCAACCAAACTGGTTTCTAACGAATTCGTGGCAATGATG
GATTTGCAGAAAGTCGCTTCTCAACTTTCACCACGCAGTGTGGGTATCCTGTCCGTGTTCTTGGTTTCATTCGCTAACTT
CTCTTCTATCGGTATCGTCGCCGGTGCGATTAAAGGCCTGAATGAGCATCAAGGTAATGTGGTTTCCCGTTTTGGTCTGA
AGCTGTTGTATGGCTCCACGTTGGTCAGTGTGCTGTCTGCTTCTATCGCGGGCTTGGTGCTCTAA

Upstream 100 bases:

>100_bases
GAAAGTGAATTTTTATATTTGTGGTTCACATCTCTTTTTTAATGTAGCCGTTACATAAAATGACCACCAAAATCTCGCCT
GCCTCAAATTTGGAGCACAT

Downstream 100 bases:

>100_bases
GTCCCCATTAACGATCGCTTAACGAGGCGCTACGGCACCTTTTTTGTATCTATAATTCAGCATCAAATTATTTTTCCGAG
TGATTATCCTCGGGGGCGAA

Product: nucleoside permease

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSHILQFALALVVVAILALAICKDRKSIRIRFVIQLLVIEVLLAYFFLHSEAGLGFVKGFAGFFDTLLGFAAAGTDFVFN
DMGDKGLAFFFLRVLCPIVFISALIGILQYIKVLPIVIRLIGTLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
MSEKRMYTMAATAMSTVSMSIVGAYMSMLDAKFVVAALVLNMFSTFIVLSLINPYKVGEEQDLQLSNLHEGQSFFEMLGE
YILAGFKVAIIVAAMLIGFIALIAAVNALFTLLFGISFQGILGYVFFPFAWVMGIPAHEALQVGSIMATKLVSNEFVAMM
DLQKVASQLSPRSVGILSVFLVSFANFSSIGIVAGAIKGLNEHQGNVVSRFGLKLLYGSTLVSVLSASIAGLVL

Sequences:

>Translated_394_residues
MSHILQFALALVVVAILALAICKDRKSIRIRFVIQLLVIEVLLAYFFLHSEAGLGFVKGFAGFFDTLLGFAAAGTDFVFN
DMGDKGLAFFFLRVLCPIVFISALIGILQYIKVLPIVIRLIGTLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
MSEKRMYTMAATAMSTVSMSIVGAYMSMLDAKFVVAALVLNMFSTFIVLSLINPYKVGEEQDLQLSNLHEGQSFFEMLGE
YILAGFKVAIIVAAMLIGFIALIAAVNALFTLLFGISFQGILGYVFFPFAWVMGIPAHEALQVGSIMATKLVSNEFVAMM
DLQKVASQLSPRSVGILSVFLVSFANFSSIGIVAGAIKGLNEHQGNVVSRFGLKLLYGSTLVSVLSASIAGLVL
>Mature_393_residues
SHILQFALALVVVAILALAICKDRKSIRIRFVIQLLVIEVLLAYFFLHSEAGLGFVKGFAGFFDTLLGFAAAGTDFVFND
MGDKGLAFFFLRVLCPIVFISALIGILQYIKVLPIVIRLIGTLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKM
SEKRMYTMAATAMSTVSMSIVGAYMSMLDAKFVVAALVLNMFSTFIVLSLINPYKVGEEQDLQLSNLHEGQSFFEMLGEY
ILAGFKVAIIVAAMLIGFIALIAAVNALFTLLFGISFQGILGYVFFPFAWVMGIPAHEALQVGSIMATKLVSNEFVAMMD
LQKVASQLSPRSVGILSVFLVSFANFSSIGIVAGAIKGLNEHQGNVVSRFGLKLLYGSTLVSVLSASIAGLVL

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=418, Percent_Identity=26.555023923445, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI11545853, Length=358, Percent_Identity=25.9776536312849, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI227116277, Length=400, Percent_Identity=23.5, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788737, Length=400, Percent_Identity=81, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI1788485, Length=411, Percent_Identity=29.9270072992701, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1788488, Length=411, Percent_Identity=28.9537712895377, Blast_Score=157, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17560276, Length=392, Percent_Identity=25.5102040816327, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71991794, Length=390, Percent_Identity=24.6153846153846, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45552519, Length=388, Percent_Identity=27.0618556701031, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45552517, Length=388, Percent_Identity=27.5773195876289, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921868, Length=388, Percent_Identity=27.5773195876289, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281360430, Length=340, Percent_Identity=25.8823529411765, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 42538; Mature: 42406

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHILQFALALVVVAILALAICKDRKSIRIRFVIQLLVIEVLLAYFFLHSEAGLGFVKGF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH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HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]