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The map label for this gene is yfhA [H]

Identifier: 218929970

GI number: 218929970

Start: 3255724

End: 3257061

Strand: Reverse

Name: yfhA [H]

Synonym: YPO2914

Alternate gene names: 218929970

Gene position: 3257061-3255724 (Counterclockwise)

Preceding gene: 218929971

Following gene: 218929969

Centisome position: 69.99

GC content: 50.0

Gene sequence:

>1338_bases
ATGACACCACGCAAACCGGCCAATCTACTGCTGGTTGACGACGATCCCAGCTTATTGAAATTGCTGGGGATGCGTTTAAC
CAGTGAAGGGTTTAATGTCACGACAGCTGAAAGCGGTCACGAAGCATTGCGTTTGTTAATGCGTGAGAAAATTGACATTG
TTATCAGTGATCTGCGAATGGATGAGATGGACGGCATGGCGCTGTTCGCTGAAATTCAAAAATATCAACCGGGGATGCCT
GTGATTATCCTCACAGCGCACGGTTCGATCCCTGATGCCGTTGCCGCGACCCAGCAAGGGGTATTCAGTTTTCTGACCAA
GCCTGTTGATCGCGATGCGCTATATAAGGCTATTGATGCGGCATTAGAACTTTCGATTCCAGCGGGTGATGATACTTGGC
GTGAAGAGATAGTGACTCGCAGCCCGGTCATGCTACGTCTGTTAGAACAAGCGAAAATGGTCGCACAATCTGACGTCAGT
GTGCTGATTAACGGGCAAAGCGGCACCGGTAAAGAGGTATTGGCACAGGCTATCCATGCCGCCAGCCCACGGGCCAAAAA
AGCCTTTATTGCCATCAACTGTGGTGCCTTACCAGAGCAGTTGCTGGAGTCTGAATTATTCGGCCATGCTAAGGGTGCGT
TTACCGGTGCAGTCAGCAGCCGTGAAGGGTTGTTTCAGGCCGCTGAAGGGGGCACATTGTTCCTGGATGAGATCGGCGAT
ATGCCGCTCTCATTACAAGTAAAATTACTGCGGGTATTGCAAGAGCGCAAAGTTCGTCCATTGGGCAGCAATCGTGATTT
AAGTATTAATGTCCGGGTGATTTCAGCGACTCACCGTGACTTGCCCAAAGCGATGGCAAAGAATGAATTCCGTGAAGATC
TCTACTACCGTTTAAATGTGGTTAACCTAAAAATCCCGGCGCTACACGAGCGGGCGGAAGATATTCCATTGCTGGCAAAC
CATCTATTACGGGAGTCGGCTAAGCGGCATAAGCCGTTTGTTCGCAGTTTCTCAAACGATGCGATGAAGCGGTTGATGAC
AGCCAGTTGGCCCGGTAACGTGCGCCAACTGGTCAACGTGATTGAGCAATGCGTGGCGTTGACCTCGGCACCGGTTATCA
GCGAAGCGCTGGTTGAGCAGGCGCTGGAGGGTGAAAATACCGTATTACCGACGTTTGTGGAGGCCCGTAATCAATTCGAA
CTAAACTATTTGCGTAAGCTATTACAAATTGCCAAAGGTAACGTTACCCAAGCCGCTCGTATGGCGGGGCGTAACCGTAC
TGAGTTCTATAAATTGTTGTCTCGCCACGAGTTGGATGCCAACGATTTTAAAGAGTAA

Upstream 100 bases:

>100_bases
AGTAAATCAGCACCACCTGTGGTAGAAAAACCGGCTGAACCGCATACACCGCCAGTGGTTTGGCCTGCAGATGTACCACC
TGCGTCTAATAAGGAATCTC

Downstream 100 bases:

>100_bases
TCATCGTTAGCTACTGACAATGATACCCAATAGATTTCAAGATGCAGACAGGCGGCCTGGTAAAGGCGGGGCCCGTGATG
GGCCAGCCAATGCCCCTGTG

Product: two-component system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTPRKPANLLLVDDDPSLLKLLGMRLTSEGFNVTTAESGHEALRLLMREKIDIVISDLRMDEMDGMALFAEIQKYQPGMP
VIILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDAALELSIPAGDDTWREEIVTRSPVMLRLLEQAKMVAQSDVS
VLINGQSGTGKEVLAQAIHAASPRAKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAEGGTLFLDEIGD
MPLSLQVKLLRVLQERKVRPLGSNRDLSINVRVISATHRDLPKAMAKNEFREDLYYRLNVVNLKIPALHERAEDIPLLAN
HLLRESAKRHKPFVRSFSNDAMKRLMTASWPGNVRQLVNVIEQCVALTSAPVISEALVEQALEGENTVLPTFVEARNQFE
LNYLRKLLQIAKGNVTQAARMAGRNRTEFYKLLSRHELDANDFKE

Sequences:

>Translated_445_residues
MTPRKPANLLLVDDDPSLLKLLGMRLTSEGFNVTTAESGHEALRLLMREKIDIVISDLRMDEMDGMALFAEIQKYQPGMP
VIILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDAALELSIPAGDDTWREEIVTRSPVMLRLLEQAKMVAQSDVS
VLINGQSGTGKEVLAQAIHAASPRAKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAEGGTLFLDEIGD
MPLSLQVKLLRVLQERKVRPLGSNRDLSINVRVISATHRDLPKAMAKNEFREDLYYRLNVVNLKIPALHERAEDIPLLAN
HLLRESAKRHKPFVRSFSNDAMKRLMTASWPGNVRQLVNVIEQCVALTSAPVISEALVEQALEGENTVLPTFVEARNQFE
LNYLRKLLQIAKGNVTQAARMAGRNRTEFYKLLSRHELDANDFKE
>Mature_444_residues
TPRKPANLLLVDDDPSLLKLLGMRLTSEGFNVTTAESGHEALRLLMREKIDIVISDLRMDEMDGMALFAEIQKYQPGMPV
IILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDAALELSIPAGDDTWREEIVTRSPVMLRLLEQAKMVAQSDVSV
LINGQSGTGKEVLAQAIHAASPRAKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAEGGTLFLDEIGDM
PLSLQVKLLRVLQERKVRPLGSNRDLSINVRVISATHRDLPKAMAKNEFREDLYYRLNVVNLKIPALHERAEDIPLLANH
LLRESAKRHKPFVRSFSNDAMKRLMTASWPGNVRQLVNVIEQCVALTSAPVISEALVEQALEGENTVLPTFVEARNQFEL
NYLRKLLQIAKGNVTQAARMAGRNRTEFYKLLSRHELDANDFKE

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=441, Percent_Identity=87.7551020408163, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI1790437, Length=439, Percent_Identity=44.4191343963554, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=41.1894273127753, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=38.8888888888889, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI87082117, Length=233, Percent_Identity=54.9356223175966, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789087, Length=308, Percent_Identity=46.1038961038961, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1789233, Length=253, Percent_Identity=47.8260869565217, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=41.3249211356467, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=39.4984326018809, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=36.9565217391304, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=41.5966386554622, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1789828, Length=253, Percent_Identity=37.5494071146245, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=32.8025477707006, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI145693157, Length=110, Percent_Identity=36.3636363636364, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=115, Percent_Identity=31.304347826087, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49322; Mature: 49190

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPRKPANLLLVDDDPSLLKLLGMRLTSEGFNVTTAESGHEALRLLMREKIDIVISDLRM
CCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCH
DEMDGMALFAEIQKYQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDA
HHCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALELSIPAGDDTWREEIVTRSPVMLRLLEQAKMVAQSDVSVLINGQSGTGKEVLAQAIHA
HHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
ASPRAKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAEGGTLFLDEIGD
CCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCCCHHEECCCCEEEEHHHCC
MPLSLQVKLLRVLQERKVRPLGSNRDLSINVRVISATHRDLPKAMAKNEFREDLYYRLNV
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHEEEE
VNLKIPALHERAEDIPLLANHLLRESAKRHKPFVRSFSNDAMKRLMTASWPGNVRQLVNV
EEEECCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IEQCVALTSAPVISEALVEQALEGENTVLPTFVEARNQFELNYLRKLLQIAKGNVTQAAR
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHH
MAGRNRTEFYKLLSRHELDANDFKE
HHCCCHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TPRKPANLLLVDDDPSLLKLLGMRLTSEGFNVTTAESGHEALRLLMREKIDIVISDLRM
CCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCH
DEMDGMALFAEIQKYQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDA
HHCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALELSIPAGDDTWREEIVTRSPVMLRLLEQAKMVAQSDVSVLINGQSGTGKEVLAQAIHA
HHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
ASPRAKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAEGGTLFLDEIGD
CCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCCCHHEECCCCEEEEHHHCC
MPLSLQVKLLRVLQERKVRPLGSNRDLSINVRVISATHRDLPKAMAKNEFREDLYYRLNV
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHEEEE
VNLKIPALHERAEDIPLLANHLLRESAKRHKPFVRSFSNDAMKRLMTASWPGNVRQLVNV
EEEECCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IEQCVALTSAPVISEALVEQALEGENTVLPTFVEARNQFELNYLRKLLQIAKGNVTQAAR
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHH
MAGRNRTEFYKLLSRHELDANDFKE
HHCCCHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]