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The map label for this gene is nadE [H]

Identifier: 218929969

GI number: 218929969

Start: 3253942

End: 3255564

Strand: Reverse

Name: nadE [H]

Synonym: YPO2912

Alternate gene names: 218929969

Gene position: 3255564-3253942 (Counterclockwise)

Preceding gene: 218929970

Following gene: 218929968

Centisome position: 69.96

GC content: 49.72

Gene sequence:

>1623_bases
ATGAGCAGATCACTTTCCATTGCGTTAGCCCAGTTGAATTGGCTGGTCGGCGATATTGAAGGCAACACTGAACGTATGTT
GCAAACCCTAAATGAGCAGCAGAAGGCGGGTGCCGATCTGGTCATGTTCTCCGAGCTGGCTTTGTCCGGTTATCCACCGG
AAGATCTTCTGTACCGTGATGACTTTTATCAACGTTGTGAAGTGCAACTTGAGCGTTTGCAAGCGGCAACCCCGCATATT
GCCGTGCTGGTGGGCCACCCATGGCGGGAAGGGGATAAGCTGTATAATGCACTGTCGCTGTTTGCACAAGGTAAACTGCT
GGGGCGCTATTTTAAACAACAACTGCCTAATTACGGCGTATTTGATGAAAAACGCTATTTCTCCGCAGGTCATCAAAGCT
GTGTCGTTGAGCTGAAAGGCTACCGCCTGGGTCTACTGATTTGTGAAGACTTATGGCTTGATGGTCCGATAGATGCAGTG
AAAGCCGCGGGCGCAGAAGTGGTATTGTCGATCAATGCCTCTCCTTATAATCGTGAAAAACCCTATATTCGTAAAACGCT
GATGGCCGCTCATTGCCAGCGTACGGGCTTACCACTGGTGTATCTCAACCAGATTGGTGGGCAGGATGAGCTGATTTTTG
ATGGCTGCTCCAAAGTCTTCGATGCGGCGGGTAACATGACCCATCGTTTGGCTGCATTTGCAGAGCAAACCACGCTGTTG
CAGTTAAATGAGTGTAACGTGATCCCGATGACGGCACCTGTGGCGGAACTGCCTCCTCTGGCTCAAGTCTATGAAGCCTT
GGTATTGGCGGTACGTGACTACGTCACCAAGAATGGCTTTAATGGGGCAGTGCTGGGTTTATCTGGCGGCATTGACTCAG
CATTGACATTGGCCATTGCGGTTGATGCGTTGGGCAAAGACAAGGTTCAGGCATTGATGATGCCATTCCGCTATACCGCA
GATATCAGTATTGCTGATGCCAAAGAAGAAGCTGAAATACTGGGTGTCAAATTTGATGTGATGTCTATCGAACCCATGTT
TGATGCTTTTATGGGCCAGTTGGCACCGATGTTTGCCGATACCGGGCGCGATACCACGGAGGAGAACCTTCAGGCTCGTT
GCCGTGGCGTGGTTCTGATGGCTTTATCGAATAAACGCCGCAGTATTGTATTAACTACGGGTAACAAAAGTGAAATGGCC
GTGGGCTACGCGACGCTGTACGGTGATATGGCGGGGGGCTTTGATGTCCTGAAAGATGTACCGAAGACGTTAGTATTCAA
ATTAGCGGAATACCGTAATACGGTCTCTTATGTGATCCCGCAGCGCGTGATCACGCGTCCGCCTTCAGCCGAATTGGCAC
CTGATCAGAAAGATGAAGATAGCCTGCCTCCTTATGATATTTTGGACGCTATCCTTGAAGGATATGTTGAACAGGATAAG
TCAGTGGCTGAGCTGGTGGCTGACGGTTTTGATGAAGCTATCGTGCGTAAAGTCATCCGCCTGGTCGACATTAATGAATA
TAAACGCCGCCAGTCAGCTGTAGGGCCACGTATTACCGCCCGCAGTTTTGGTAAAGATAGACGCTATCCGATCACATCAG
GCTTTGGCCGCAAGAATTGGTAA

Upstream 100 bases:

>100_bases
GTAAAGGCGGGGCCCGTGATGGGCCAGCCAATGCCCCTGTGGTTTTGAAAGATGAGGGTATATTATTCCCTGACAATGCT
GAAACAGAGTTTAAACACCA

Downstream 100 bases:

>100_bases
AACAGGGATTCACCATGAAAAAAATTGACGCGATTATTAAACCATTCAAACTGGATGACGTACGTGAAGCGCTGGCTGAA
GTCGGTATTACCGGGATGAC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRDDFYQRCEVQLERLQAATPHI
AVLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGVFDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAV
KAAGAEVVLSINASPYNREKPYIRKTLMAAHCQRTGLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLL
QLNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTA
DISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFADTGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMA
VGYATLYGDMAGGFDVLKDVPKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDK
SVAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW

Sequences:

>Translated_540_residues
MSRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRDDFYQRCEVQLERLQAATPHI
AVLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGVFDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAV
KAAGAEVVLSINASPYNREKPYIRKTLMAAHCQRTGLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLL
QLNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTA
DISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFADTGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMA
VGYATLYGDMAGGFDVLKDVPKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDK
SVAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW
>Mature_539_residues
SRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRDDFYQRCEVQLERLQAATPHIA
VLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGVFDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAVK
AAGAEVVLSINASPYNREKPYIRKTLMAAHCQRTGLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLLQ
LNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTAD
ISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFADTGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAV
GYATLYGDMAGGFDVLKDVPKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDKS
VAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321865, Length=590, Percent_Identity=23.3898305084746, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59794; Mature: 59663

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRD
CCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCHHCCCHH
DFYQRCEVQLERLQAATPHIAVLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGV
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAVKAAGAEVVLSINASPYNREK
CCCHHHHHCCCCEEEEEECCCEEEEEEEEHHCCCCCHHHHHHCCCEEEEEECCCCCCCCC
PYIRKTLMAAHCQRTGLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLL
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCEEE
QLNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIA
EECCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
VDALGKDKVQALMMPFRYTADISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFAD
HHHCCHHHHHHHHHCCEEECCEEEECCCCCHHEEEEEEEEEECCHHHHHHHHHHHHHHHC
TGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAVGYATLYGDMAGGFDVLKDV
CCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHCCCCCHHHHHHH
PKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCH
SVAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRD
CCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCHHCCCHH
DFYQRCEVQLERLQAATPHIAVLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGV
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAVKAAGAEVVLSINASPYNREK
CCCHHHHHCCCCEEEEEECCCEEEEEEEEHHCCCCCHHHHHHCCCEEEEEECCCCCCCCC
PYIRKTLMAAHCQRTGLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLL
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCEEE
QLNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIA
EECCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
VDALGKDKVQALMMPFRYTADISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFAD
HHHCCHHHHHHHHHCCEEECCEEEECCCCCHHEEEEEEEEEECCHHHHHHHHHHHHHHHC
TGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAVGYATLYGDMAGGFDVLKDV
CCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHCCCCCHHHHHHH
PKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCH
SVAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW
HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]