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The map label for this gene is trmJ [H]

Identifier: 218929957

GI number: 218929957

Start: 3240880

End: 3241653

Strand: Reverse

Name: trmJ [H]

Synonym: YPO2898

Alternate gene names: 218929957

Gene position: 3241653-3240880 (Counterclockwise)

Preceding gene: 218929960

Following gene: 218929956

Centisome position: 69.66

GC content: 50.26

Gene sequence:

>774_bases
ATGCTACACAACATTCGTATTGTCTTGGTTGAAACTTCACACACCGGCAACATGGGCTCGACCGCCAGAGCCATGAAAAC
AATGGGATTAACCAATCTATATTTGGTTAATCCATTGGTAAAACCAGATTCTCAGGCCATTGCTTTGTCAGCCGGTGCCA
GTGATGTTATTGGTAAGGCGACTATCGTTGATACGTTAGATGAAGCCCTTGCCGGATGTAGTTTGGTGGTCGGCACCAGC
GCCCGTTCCAGAACGTTACCTTGGCCAATGCTGGAACCGCGTGAATGCGGTGTACGCAGTGCGCGCGAAGCTGAACATGC
TCCGGTTGCCTTGGTGTTTGGTCGCGAGCGTGTTGGCCTGACTAACGACGAGCTGCAAAAATGCCATTATCATGTGGCTA
TCCCCGCCAATCCTGAATATAGCTCCTTAAATTTAGCCATGGCCGTGCAAATTTTGGCTTATGAAGTGCGGGTGGCATAC
CTTGATCGTCAGCAGGCAAATGCCCCTGTAGAAGAAGAGGAAGAGGCACCTTATCCTTTGGTGGATGATCTGGAGCGTTT
CTATCAGCATTTGGAGCAGGTTTTGTCTCATAGTGGCTTTATCCGCCAGGCCCATCCGGGCCAGATTATGAGCAAATTAA
GGCGCCTCTTTACCCGCGCTCGGCCCGAGGCTCAAGAGCTGAATATTCTACGTGGGATGCTGACATCCATCGAAAAGCAG
GATAAATATCCACAACGCGGTACCGGTGATACGGCGGGTAAGAGTAAGGATTAA

Upstream 100 bases:

>100_bases
TGTTAAAGAACGAGAAACGGCGCACAGTATAGCAGGGGATCTGATAAATGCCTATGTCTGTGTTAAGATATGGCGATTAT
CCGTTATCCTCAGAGTTTGT

Downstream 100 bases:

>100_bases
TGTCTTATATCAATTGGTTATAATTAATTCTAATTGATACTATCGAGCGTAAGTATGGTGATAATAGTTGAGTGAATTAC
TAGGTTAAATAGTTGACTAA

Product: putative SpoU family rRNA methylase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVKPDSQAIALSAGASDVIGKATIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLEPRECGVRSAREAEHAPVALVFGRERVGLTNDELQKCHYHVAIPANPEYSSLNLAMAVQILAYEVRVAY
LDRQQANAPVEEEEEAPYPLVDDLERFYQHLEQVLSHSGFIRQAHPGQIMSKLRRLFTRARPEAQELNILRGMLTSIEKQ
DKYPQRGTGDTAGKSKD

Sequences:

>Translated_257_residues
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVKPDSQAIALSAGASDVIGKATIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLEPRECGVRSAREAEHAPVALVFGRERVGLTNDELQKCHYHVAIPANPEYSSLNLAMAVQILAYEVRVAY
LDRQQANAPVEEEEEAPYPLVDDLERFYQHLEQVLSHSGFIRQAHPGQIMSKLRRLFTRARPEAQELNILRGMLTSIEKQ
DKYPQRGTGDTAGKSKD
>Mature_257_residues
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVKPDSQAIALSAGASDVIGKATIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLEPRECGVRSAREAEHAPVALVFGRERVGLTNDELQKCHYHVAIPANPEYSSLNLAMAVQILAYEVRVAY
LDRQQANAPVEEEEEAPYPLVDDLERFYQHLEQVLSHSGFIRQAHPGQIMSKLRRLFTRARPEAQELNILRGMLTSIEKQ
DKYPQRGTGDTAGKSKD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=242, Percent_Identity=81.8181818181818, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1790865, Length=161, Percent_Identity=34.7826086956522, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28386; Mature: 28386

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVKPDSQAIALSAGASDVIGKA
CCCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCHHHHHH
TIVDTLDEALAGCSLVVGTSARSRTLPWPMLEPRECGVRSAREAEHAPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCCCHHHHHCCCCEEEEECCCCCCC
TNDELQKCHYHVAIPANPEYSSLNLAMAVQILAYEVRVAYLDRQQANAPVEEEEEAPYPL
CHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
VDDLERFYQHLEQVLSHSGFIRQAHPGQIMSKLRRLFTRARPEAQELNILRGMLTSIEKQ
HHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DKYPQRGTGDTAGKSKD
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVKPDSQAIALSAGASDVIGKA
CCCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCHHHHHH
TIVDTLDEALAGCSLVVGTSARSRTLPWPMLEPRECGVRSAREAEHAPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCCCHHHHHCCCCEEEEECCCCCCC
TNDELQKCHYHVAIPANPEYSSLNLAMAVQILAYEVRVAYLDRQQANAPVEEEEEAPYPL
CHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
VDDLERFYQHLEQVLSHSGFIRQAHPGQIMSKLRRLFTRARPEAQELNILRGMLTSIEKQ
HHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DKYPQRGTGDTAGKSKD
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA