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The map label for this gene is ispG

Identifier: 218929939

GI number: 218929939

Start: 3214899

End: 3216026

Strand: Reverse

Name: ispG

Synonym: YPO2879

Alternate gene names: 218929939

Gene position: 3216026-3214899 (Counterclockwise)

Preceding gene: 218929940

Following gene: 218929938

Centisome position: 69.11

GC content: 45.57

Gene sequence:

>1128_bases
ATGCATAACGGATCCCCTATTATTCGGCGTAAATCGACCCGGATTTACGTCGGTAAAGTGCCTATTGGTGATGGCGCACC
GATTGCTGTGCAATCAATGACCAATACCAAAACCACGGATGTGGATGCCACTGTTGCCCAAATTAAAGCATTGGAACGTG
TTGGTGTGGATATCGTGCGGGTCTCGATACCGACAATGGATGCAGCTGAAGCCTTTAAGTTAATCAAACAGCAGTCTACT
GTTCCGCTAGTCGCAGACATTCATTTTGATTACCGAATCGCCTTGAAAGTGGCTGAGTATGGTGTCGATTGTTTGCGGAT
CAACCCCGGTAATATTGGTAATGAATCACGTATTCGTGAAGTTGTTGCCTGTGCACGTGATTACAATATCCCTATTCGTA
TTGGTATCAATGGCGGCTCGCTGGAAAAAGATATTCAGGAGAAATATGGTGAACCTACACCAGAAGCCCTGCTTGAATCC
GCGATGCGTCATGTCGATATCCTTGATCGTCTCAATTTCGATCAGTTTAAAGTCAGTGTTAAAGCCTCCGATGTCTTCCT
GGCCGTCAATTCCTATCGTTTGCTAGCGAAACAAATTAATAATCCTCTGCATTTGGGGATCACTGAAGCGGGTGGTGCGC
GCAGTGGTTCGGTTAAATCTGCGATTGGATTAGGTTTGCTGTTATCGGAAGGGATTGGCGATACTTTGCGGATCTCTTTG
GCCGCAGATCCAGTAGAAGAAGTGAAAGTAGGCTTCGATATCCTTAAATCACTGCGTATTCGTGCGCGTGGGATTAATTT
CATCGCTTGCCCAACCTGTTCTCGTCAAGAGTTTGATGTTATTGGGACGGTGAATGCCTTGGAACAACGGCTGGAAGACC
TGATTACACCTATGGATGTTTCTATCATCGGTTGTGTGGTTAATGGTCCGGGAGAAGCGTTGGTGTCGACAATTGGTGTC
ACTGGTGCGCGTAACCACAGTGGTTTTTACGAAGACGGGGTGCGTCAAAAAGAGCGCTTTGATAACAAAGCCATGATTGA
CCAATTGGAAGCAAAAATTCGTGCCAAAGCATCAATTCTTGATGCGAATAACCGGATTGTTATCAACCAATTGGATGATA
ATAAATAG

Upstream 100 bases:

>100_bases
GCTAATCGCGTAGCCCGCCTGACTGTCTGTGTAGAGTAGAATGATGGTTTTGACAAATATCATGTTGTAAGAACTACACG
ACCGTAAAGGAGAGTATGTA

Downstream 100 bases:

>100_bases
GCTCTATCGGCAATCCTTGTTGTCGGCCAGAATCAAACAGACAGTTATGCTGTGACCGTGTTTTATATTGTGCCGATAGT
GTTTTATATTGTGCTAATAG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MHNGSPIIRRKSTRIYVGKVPIGDGAPIAVQSMTNTKTTDVDATVAQIKALERVGVDIVRVSIPTMDAAEAFKLIKQQST
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIREVVACARDYNIPIRIGINGGSLEKDIQEKYGEPTPEALLES
AMRHVDILDRLNFDQFKVSVKASDVFLAVNSYRLLAKQINNPLHLGITEAGGARSGSVKSAIGLGLLLSEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDLITPMDVSIIGCVVNGPGEALVSTIGV
TGARNHSGFYEDGVRQKERFDNKAMIDQLEAKIRAKASILDANNRIVINQLDDNK

Sequences:

>Translated_375_residues
MHNGSPIIRRKSTRIYVGKVPIGDGAPIAVQSMTNTKTTDVDATVAQIKALERVGVDIVRVSIPTMDAAEAFKLIKQQST
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIREVVACARDYNIPIRIGINGGSLEKDIQEKYGEPTPEALLES
AMRHVDILDRLNFDQFKVSVKASDVFLAVNSYRLLAKQINNPLHLGITEAGGARSGSVKSAIGLGLLLSEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDLITPMDVSIIGCVVNGPGEALVSTIGV
TGARNHSGFYEDGVRQKERFDNKAMIDQLEAKIRAKASILDANNRIVINQLDDNK
>Mature_375_residues
MHNGSPIIRRKSTRIYVGKVPIGDGAPIAVQSMTNTKTTDVDATVAQIKALERVGVDIVRVSIPTMDAAEAFKLIKQQST
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIREVVACARDYNIPIRIGINGGSLEKDIQEKYGEPTPEALLES
AMRHVDILDRLNFDQFKVSVKASDVFLAVNSYRLLAKQINNPLHLGITEAGGARSGSVKSAIGLGLLLSEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDLITPMDVSIIGCVVNGPGEALVSTIGV
TGARNHSGFYEDGVRQKERFDNKAMIDQLEAKIRAKASILDANNRIVINQLDDNK

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=372, Percent_Identity=86.8279569892473, Blast_Score=654, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_YERP3 (A7FFY9)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400167.1
- ProteinModelPortal:   A7FFY9
- STRING:   A7FFY9
- GeneID:   5385400
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1186
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   YPSE349747:YPSIP31758_1186-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40798; Mature: 40798

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNGSPIIRRKSTRIYVGKVPIGDGAPIAVQSMTNTKTTDVDATVAQIKALERVGVDIVR
CCCCCCCEEECCCEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEE
VSIPTMDAAEAFKLIKQQSTVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRE
EECCCCCHHHHHHHHHHCCCCCEEEEEECCEEEEEEEECCCCEEEEECCCCCCCHHHHHH
VVACARDYNIPIRIGINGGSLEKDIQEKYGEPTPEALLESAMRHVDILDRLNFDQFKVSV
HHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVNSYRLLAKQINNPLHLGITEAGGARSGSVKSAIGLGLLLSEGIGDTLRISL
EECEEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDLITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSTIGVTGARNHSGFYEDGVRQKERFDNKAMIDQLEAKIRAKASIL
EEEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEE
DANNRIVINQLDDNK
ECCCEEEEEECCCCC
>Mature Secondary Structure
MHNGSPIIRRKSTRIYVGKVPIGDGAPIAVQSMTNTKTTDVDATVAQIKALERVGVDIVR
CCCCCCCEEECCCEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEE
VSIPTMDAAEAFKLIKQQSTVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNESRIRE
EECCCCCHHHHHHHHHHCCCCCEEEEEECCEEEEEEEECCCCEEEEECCCCCCCHHHHHH
VVACARDYNIPIRIGINGGSLEKDIQEKYGEPTPEALLESAMRHVDILDRLNFDQFKVSV
HHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVNSYRLLAKQINNPLHLGITEAGGARSGSVKSAIGLGLLLSEGIGDTLRISL
EECEEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDLITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSTIGVTGARNHSGFYEDGVRQKERFDNKAMIDQLEAKIRAKASIL
EEEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEE
DANNRIVINQLDDNK
ECCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA