Click here to switch to the map view.

The map label for this gene is truA

Identifier: 218929838

GI number: 218929838

Start: 3100249

End: 3101043

Strand: Direct

Name: truA

Synonym: YPO2766

Alternate gene names: 218929838

Gene position: 3100249-3101043 (Clockwise)

Preceding gene: 218929837

Following gene: 218929839

Centisome position: 66.62

GC content: 51.57

Gene sequence:

>795_bases
TTGAAAATTGCGTTGGGTATTGAGTATAACGGTAGCCGCTATTTTGGTTGGCAACGGCAGCAAGAGGTTGCCAGCGTGCA
GGCCTGTCTTGAGGCGGCATTATCCAAAGTGGCTAATGAACCCATTGGGGTCTTCTGCGCTGGCCGTACCGATGCAGGGG
TCCATGCTACCGGGCAAGTTGTGCACTTTGTTACCTCTGCAGTACGTAAAGACGCCGCCTGGACTATGGGGGTCAACAGC
CATTTGCCCGCGGATATCGCAGTGCGTTGGGTCAAAACCGTCGATAACGATTTTCATGCCCGCTTTAGCGCAACGGCCCG
CCGCTATCGCTATATCATTTTTAGCCACCGCTATCGCCCTGCGATATTGGCGCAAGGGGTCACCCATTGCTATATGCCAT
TGGATGCAGAAAAAATGGAACGCGCCGCGCAATGTTTACTCGGCGAAAATGATTTTACCTCTTTCCGTGCCGTACAATGT
CAATCCCGCACGCCATGGAGAAATGTAAAACATGTCAAAGTTACCCGACATGGCGCGTATATCGTGGTAGATATCAAGGC
CAACGCGTTTGTACATCATATGGTGCGCAATATCGTTGGGAGCCTGATAGAAATTGGTTGTGGCAATCAGGACGTGACCT
GGATGGCAGAATTGTTGGCGTTGAAGGACAGAACACGTGCTGCCGCAACCGCCAAAGCCGATGGGTTGTATTTAGTGTCT
GTCGATTACCCGGACCACTTCGCGTTACCCAAAGTTCCTATGGGGCCTTTGTTTTTGGCGGATGACGAGGGTTAA

Upstream 100 bases:

>100_bases
AGCAACTATACTGATGTCCGGGTTTGAATTAGATCAGGAAGAAACATTAGTTCAGCAAAACACATTGGCTCAGCAAGAAA
AACCGGCAGAAGTGGCCGGT

Downstream 100 bases:

>100_bases
CGGTTCACCGGTGACTAAAGATACCGAGAGCCAATTGTCAGGTATGTCGATGGCAAACTGAGGCGTCGCTTTGGGCTAAG
GAATACCTAAGGAAATTCAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVFCAGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNS
HLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCYMPLDAEKMERAAQCLLGENDFTSFRAVQC
QSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEIGCGNQDVTWMAELLALKDRTRAAATAKADGLYLVS
VDYPDHFALPKVPMGPLFLADDEG

Sequences:

>Translated_264_residues
MKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVFCAGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNS
HLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCYMPLDAEKMERAAQCLLGENDFTSFRAVQC
QSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEIGCGNQDVTWMAELLALKDRTRAAATAKADGLYLVS
VDYPDHFALPKVPMGPLFLADDEG
>Mature_264_residues
MKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVFCAGRTDAGVHATGQVVHFVTSAVRKDAAWTMGVNS
HLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRPAILAQGVTHCYMPLDAEKMERAAQCLLGENDFTSFRAVQC
QSRTPWRNVKHVKVTRHGAYIVVDIKANAFVHHMVRNIVGSLIEIGCGNQDVTWMAELLALKDRTRAAATAKADGLYLVS
VDYPDHFALPKVPMGPLFLADDEG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=284, Percent_Identity=29.5774647887324, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=80.3846153846154, Blast_Score=446, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_YERP3 (A7FGM1)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400399.1
- ProteinModelPortal:   A7FGM1
- SMR:   A7FGM1
- STRING:   A7FGM1
- GeneID:   5384532
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1420
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- ProtClustDB:   PRK00021
- BioCyc:   YPSE349747:YPSIP31758_1420-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29348; Mature: 29348

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: ACT_SITE 51-51 BINDING 109-109 BINDING 236-236

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVFCAGRTDAGVHATGQV
CEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
VHFVTSAVRKDAAWTMGVNSHLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRP
HHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEECCCCH
AILAQGVTHCYMPLDAEKMERAAQCLLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAY
HHHHCCCHHEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCEE
IVVDIKANAFVHHMVRNIVGSLIEIGCGNQDVTWMAELLALKDRTRAAATAKADGLYLVS
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEE
VDYPDHFALPKVPMGPLFLADDEG
ECCCCCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure
MKIALGIEYNGSRYFGWQRQQEVASVQACLEAALSKVANEPIGVFCAGRTDAGVHATGQV
CEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHH
VHFVTSAVRKDAAWTMGVNSHLPADIAVRWVKTVDNDFHARFSATARRYRYIIFSHRYRP
HHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEECCCCH
AILAQGVTHCYMPLDAEKMERAAQCLLGENDFTSFRAVQCQSRTPWRNVKHVKVTRHGAY
HHHHCCCHHEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCEE
IVVDIKANAFVHHMVRNIVGSLIEIGCGNQDVTWMAELLALKDRTRAAATAKADGLYLVS
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEE
VDYPDHFALPKVPMGPLFLADDEG
ECCCCCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA