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The map label for this gene is pdxB [H]

Identifier: 218929835

GI number: 218929835

Start: 3097144

End: 3098271

Strand: Direct

Name: pdxB [H]

Synonym: YPO2763

Alternate gene names: 218929835

Gene position: 3097144-3098271 (Clockwise)

Preceding gene: 218929834

Following gene: 218929837

Centisome position: 66.55

GC content: 54.08

Gene sequence:

>1128_bases
GTGAAAATTCTGGTTGATGAAAATATGCCGTACGCTGAGGAGCTGTTCCGGCGTTTAGGTGATGTACAGGCTGTGCCAGG
GCGTCCCATTCCTCGTGACGCGTTGGTTGATGCCGATGCTCTGATGGTACGTTCGGTCACCAAAGTGAACGAGGCGCTGC
TACACGGTACCTCAATTGGTTTTGTTGGCACGGCGACGGCGGGTACCGACCATGTCGATGACACTTGGCTGCGTCAGCAG
GGGATCGGTTTCTCTGCGGCCCCCGGTTGTAACGCCATCGCCGTGGTTGAATATGTTTTCTCTGCGTTGATGATGATGGC
TGAACGGGATGGCTTCCAGTTGCGCGATAAAACCGTGGGCATTATCGGCGTCGGAAATGTGGGTTCACGGCTGAATGCTC
GTTTGCAAGCCTTAGGTGTTCGGACCTTGTTGTGCGACCCCCCGCGTGCTGATCGTGGTGATAACGAAGCCTTCTGGCCG
CTGGAAAAACTGGTACGTGAGGCTGACGTTCTGACCTTCCATACTCCGCTGAATAAAACCGGAGCCTACCAAAGTCTGCA
TATGGCCGATGATGAACTGCTGGCCGCCTTACCGGATGGCCGTATTCTGATCAACGCCTGCCGGGGTGCGGTGGTGGATA
ACGCAGCACTATTACGGGCGCTGGAAAAAGGGAAAAAACTCAGCGTGGTATTGGACGTCTGGGAGCCAGAACCGGATCTC
TCTTTACCGCTGTTGGCCCGTGTCGATATTGGCACACCGCATATTGCCGGATATACGCTAGAAGGCAAAGCACGGGGTAC
CACACAGGTTTTTGAGGCGTTCAGTCAGCATTTGGGGCAACCACAGTCGGTCGAACTGGCCTCTTTATTGCCAGTGCCAG
AGTTTAGCCATTTACGTCTAAATGGTGAGTTGGATGAAGGCAAATTAAAACGATTGATGCACTTAGTGTATGATGTGCGC
CGCGATGACGCGCCATTGCGCCATGTCGCTGGCCTGCCAGGGGAGTTTGACCGCCTGCGCAAGCATTATCAGGAGCGGCG
TGAATGGTCATCACTGTGTGTACAGTGCGATGATGCTACCAGTGCTGGACTCTTGCAGCAGCTAGGTTTTACTACTCAAT
TGCTGTGA

Upstream 100 bases:

>100_bases
ATATCGGGTACCACGCCTGAGCGTTTCCGGCGGGGCAATAGTGGCTTATACTAAGCCCCCTGTTTTTCATCTGTATGGCA
GTTCGCTGTCGGAGAGTAAA

Downstream 100 bases:

>100_bases
CGATAGGGTAGTACGAATATCACGCGTAAAAATTAAATAAATATCCTCCGGCATAGCCGGAGGTTTTTCATATGCGCCTA
TAAGGCTCTGTTACCAGCCG

Product: putative D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKILVDENMPYAEELFRRLGDVQAVPGRPIPRDALVDADALMVRSVTKVNEALLHGTSIGFVGTATAGTDHVDDTWLRQQ
GIGFSAAPGCNAIAVVEYVFSALMMMAERDGFQLRDKTVGIIGVGNVGSRLNARLQALGVRTLLCDPPRADRGDNEAFWP
LEKLVREADVLTFHTPLNKTGAYQSLHMADDELLAALPDGRILINACRGAVVDNAALLRALEKGKKLSVVLDVWEPEPDL
SLPLLARVDIGTPHIAGYTLEGKARGTTQVFEAFSQHLGQPQSVELASLLPVPEFSHLRLNGELDEGKLKRLMHLVYDVR
RDDAPLRHVAGLPGEFDRLRKHYQERREWSSLCVQCDDATSAGLLQQLGFTTQLL

Sequences:

>Translated_375_residues
MKILVDENMPYAEELFRRLGDVQAVPGRPIPRDALVDADALMVRSVTKVNEALLHGTSIGFVGTATAGTDHVDDTWLRQQ
GIGFSAAPGCNAIAVVEYVFSALMMMAERDGFQLRDKTVGIIGVGNVGSRLNARLQALGVRTLLCDPPRADRGDNEAFWP
LEKLVREADVLTFHTPLNKTGAYQSLHMADDELLAALPDGRILINACRGAVVDNAALLRALEKGKKLSVVLDVWEPEPDL
SLPLLARVDIGTPHIAGYTLEGKARGTTQVFEAFSQHLGQPQSVELASLLPVPEFSHLRLNGELDEGKLKRLMHLVYDVR
RDDAPLRHVAGLPGEFDRLRKHYQERREWSSLCVQCDDATSAGLLQQLGFTTQLL
>Mature_375_residues
MKILVDENMPYAEELFRRLGDVQAVPGRPIPRDALVDADALMVRSVTKVNEALLHGTSIGFVGTATAGTDHVDDTWLRQQ
GIGFSAAPGCNAIAVVEYVFSALMMMAERDGFQLRDKTVGIIGVGNVGSRLNARLQALGVRTLLCDPPRADRGDNEAFWP
LEKLVREADVLTFHTPLNKTGAYQSLHMADDELLAALPDGRILINACRGAVVDNAALLRALEKGKKLSVVLDVWEPEPDL
SLPLLARVDIGTPHIAGYTLEGKARGTTQVFEAFSQHLGQPQSVELASLLPVPEFSHLRLNGELDEGKLKRLMHLVYDVR
RDDAPLRHVAGLPGEFDRLRKHYQERREWSSLCVQCDDATSAGLLQQLGFTTQLL

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=303, Percent_Identity=28.3828382838284, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI145580578, Length=273, Percent_Identity=24.1758241758242, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4557499, Length=273, Percent_Identity=24.1758241758242, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI61743967, Length=155, Percent_Identity=33.5483870967742, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4557497, Length=155, Percent_Identity=33.5483870967742, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI145580575, Length=175, Percent_Identity=30.2857142857143, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788660, Length=371, Percent_Identity=72.5067385444744, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1787645, Length=206, Percent_Identity=27.1844660194175, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789279, Length=310, Percent_Identity=26.7741935483871, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082289, Length=159, Percent_Identity=32.7044025157233, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=263, Percent_Identity=26.615969581749, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6322116, Length=327, Percent_Identity=28.1345565749235, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320925, Length=327, Percent_Identity=27.82874617737, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=293, Percent_Identity=24.9146757679181, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324964, Length=259, Percent_Identity=25.0965250965251, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921140, Length=295, Percent_Identity=28.4745762711864, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28574286, Length=231, Percent_Identity=26.8398268398268, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24646446, Length=228, Percent_Identity=24.1228070175439, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646448, Length=228, Percent_Identity=24.1228070175439, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646452, Length=228, Percent_Identity=24.1228070175439, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646450, Length=228, Percent_Identity=24.1228070175439, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI62472511, Length=228, Percent_Identity=24.1228070175439, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 41168; Mature: 41168

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVDENMPYAEELFRRLGDVQAVPGRPIPRDALVDADALMVRSVTKVNEALLHGTSIG
CEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
FVGTATAGTDHVDDTWLRQQGIGFSAAPGCNAIAVVEYVFSALMMMAERDGFQLRDKTVG
EEEECCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCEEE
IIGVGNVGSRLNARLQALGVRTLLCDPPRADRGDNEAFWPLEKLVREADVLTFHTPLNKT
EEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCC
GAYQSLHMADDELLAALPDGRILINACRGAVVDNAALLRALEKGKKLSVVLDVWEPEPDL
CCCCCHHCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
SLPLLARVDIGTPHIAGYTLEGKARGTTQVFEAFSQHLGQPQSVELASLLPVPEFSHLRL
CCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEE
NGELDEGKLKRLMHLVYDVRRDDAPLRHVAGLPGEFDRLRKHYQERREWSSLCVQCDDAT
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHEEECCCCC
SAGLLQQLGFTTQLL
HHHHHHHHCCHHHCC
>Mature Secondary Structure
MKILVDENMPYAEELFRRLGDVQAVPGRPIPRDALVDADALMVRSVTKVNEALLHGTSIG
CEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
FVGTATAGTDHVDDTWLRQQGIGFSAAPGCNAIAVVEYVFSALMMMAERDGFQLRDKTVG
EEEECCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCEEE
IIGVGNVGSRLNARLQALGVRTLLCDPPRADRGDNEAFWPLEKLVREADVLTFHTPLNKT
EEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCC
GAYQSLHMADDELLAALPDGRILINACRGAVVDNAALLRALEKGKKLSVVLDVWEPEPDL
CCCCCHHCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
SLPLLARVDIGTPHIAGYTLEGKARGTTQVFEAFSQHLGQPQSVELASLLPVPEFSHLRL
CCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEE
NGELDEGKLKRLMHLVYDVRRDDAPLRHVAGLPGEFDRLRKHYQERREWSSLCVQCDDAT
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHEEECCCCC
SAGLLQQLGFTTQLL
HHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA