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The map label for this gene is lepA [H]

Identifier: 218929788

GI number: 218929788

Start: 3043412

End: 3045211

Strand: Direct

Name: lepA [H]

Synonym: YPO2716

Alternate gene names: 218929788

Gene position: 3043412-3045211 (Clockwise)

Preceding gene: 218929786

Following gene: 218929789

Centisome position: 65.4

GC content: 45.67

Gene sequence:

>1800_bases
ATGAAGCACATAAGAAACTTTTCTATTATTGCCCATATTGACCACGGTAAGTCGACGCTGTCTGATCGGATTATTCAGAT
CTGTGGCGGTTTATCTGAGCGTGAAATGGCCGCACAGGTACTGGATTCCATGGATCTAGAACGTGAGCGTGGTATTACCA
TCAAGGCACAGAGTGTGACGCTTGATTACCATTCGAAGGATGGTCAAACCTATCAACTTAACTTTATCGATACCCCCGGC
CATGTTGACTTCTCTTATGAAGTTTCACGCTCTTTAGCCGCGTGTGAAGGGGCTCTATTGGTTGTGGATGCAGGGCAAGG
CGTTGAGGCTCAGACGCTAGCAAACTGCTACACCGCGATGGAGATGGACCTGGAAGTTGTTCCGGTTCTGAACAAAATTG
ATTTGCCTGCTGCCGATCCTGAGCGGGTTGCTGAAGAAATCGAAGACATTGTGGGGATTGATGCCACTGATGCGATTCGT
TGCTCGGCAAAAACCGGTGTGGGCGTGCCTGATGTTCTTGAGCGGTTGGTCCGCGATATTCCAGCCCCTGAGGGGGATCC
AAATGGGCCATTGCAGGCATTGATTATCGATTCCTGGTTTGATAACTACCTGGGTGTTGTGTCATTAATACGTATCAAGA
ATGGTTCGTTGCGTAAAGGCGATAAAGTTAAGGTTATGAGTACCGGCCAGAGCTATAACGCGGATCGTTTAGGGATATTT
ACACCAAAACGTGTTGATCGTGATGTTCTGAACTGCGGCGAAGTAGGCTGGTTGGTTTGTGCAATAAAAGACATTCTTGG
CGCACCTGTTGGCGATACATTGACATTAACGCGTAACCCGGCAGAAAAATCATTGCCTGGCTTTAAGAAAGTAAAACCAC
AAGTTTATGCGGGCCTGTTCCCGATAAGCTCTGATGATTATGAATCTTTCCGGGATGCGTTAGGTAAGTTAAGTCTTAAC
GATGCCTCTTTGTTCTATGAACCAGAAAGCTCTACAGCATTAGGCTTTGGTTTCCGATGCGGCTTCCTTGGCTTGTTACA
TATGGAGATCATCCAGGAGCGTCTGGAGCGTGAATATGATCTGGAACTGATTACTACGGCACCAACAGTGGTGTACGAGG
TGATTACGACTAATCAGGAAACGGTCTATGTCGATAGCCCTTCTAAACTGCCTGCGTTGAACAATATTGAAGAACTGCGC
GAACCGATCGCTGAATGCCATATGTTGTTGCCACAGGAATACCTCGGTAACGTCATTACATTGTGTATCGAAAAACGTGG
TACACAGACCAATATGGTTTATCACGGTAAGCAAGTCGCGCTGACATATGAAATTCCAATGGCGGAAGTCGTGCTTGATT
TCTTTGATCGTTTGAAATCAACGTCACGGGGTTATGCTTCACTGGATTATAATTTCAAACGCTTCCAGACGTCTGACATG
GTACGTGTTGATGTATTAATCAATAACGAACGTGTGGATGCGCTGGCACTGATCACGCATCGTGATAATGCACAATATCG
TGGCCGTGATTTGGTTGAGAAAATGAAAGAACTGATCCCACGTCAACAATTTGATATTGCGATCCAGGCTGCGATTGGTA
ACCACATCATTGCTCGCTCAACGGTAAAACAGCTACGTAAAAACGTATTGGCGAAGTGTTATGGTGGGGATGTTAGCCGT
AAGAAAAAACTTCTGCAGAAACAAAAAGACGGTAAGAAACGTATGAAGCAAGTCGGTAACGTTGAATTACCACAAGAGGC
CTTCCTGGCTATTCTGCATGTTGGAAAAGACAGTAAATAA

Upstream 100 bases:

>100_bases
AAGATGCGTGAATATTTATGTGGGTAGTTTTGGGTTTGAATCCATCGCTGTGTCCACCAATAATTAATTAAAGACAACGA
CTAGAGAAAAATTCATATAA

Downstream 100 bases:

>100_bases
GGAGTTTTCATGGCTAACATGTTTGCTTTGATTCTGGCAATAGCAACGCTGTTGACGGGGATTATCTGGTGCTTCGAGCG
GTTTAAATGGGGGCCAGCCC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLSEREMAAQVLDSMDLERERGITIKAQSVTLDYHSKDGQTYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAIR
CSAKTGVGVPDVLERLVRDIPAPEGDPNGPLQALIIDSWFDNYLGVVSLIRIKNGSLRKGDKVKVMSTGQSYNADRLGIF
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLTRNPAEKSLPGFKKVKPQVYAGLFPISSDDYESFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLELITTAPTVVYEVITTNQETVYVDSPSKLPALNNIEELR
EPIAECHMLLPQEYLGNVITLCIEKRGTQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
VRVDVLINNERVDALALITHRDNAQYRGRDLVEKMKELIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDSK

Sequences:

>Translated_599_residues
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLSEREMAAQVLDSMDLERERGITIKAQSVTLDYHSKDGQTYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAIR
CSAKTGVGVPDVLERLVRDIPAPEGDPNGPLQALIIDSWFDNYLGVVSLIRIKNGSLRKGDKVKVMSTGQSYNADRLGIF
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLTRNPAEKSLPGFKKVKPQVYAGLFPISSDDYESFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLELITTAPTVVYEVITTNQETVYVDSPSKLPALNNIEELR
EPIAECHMLLPQEYLGNVITLCIEKRGTQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
VRVDVLINNERVDALALITHRDNAQYRGRDLVEKMKELIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDSK
>Mature_599_residues
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLSEREMAAQVLDSMDLERERGITIKAQSVTLDYHSKDGQTYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAIR
CSAKTGVGVPDVLERLVRDIPAPEGDPNGPLQALIIDSWFDNYLGVVSLIRIKNGSLRKGDKVKVMSTGQSYNADRLGIF
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLTRNPAEKSLPGFKKVKPQVYAGLFPISSDDYESFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLELITTAPTVVYEVITTNQETVYVDSPSKLPALNNIEELR
EPIAECHMLLPQEYLGNVITLCIEKRGTQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
VRVDVLINNERVDALALITHRDNAQYRGRDLVEKMKELIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDSK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=46.2562396006656, Blast_Score=588, Evalue=1e-168,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=44.0298507462687, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=42.9530201342282, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI25306287, Length=149, Percent_Identity=42.9530201342282, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI19923640, Length=149, Percent_Identity=42.9530201342282, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=41.6666666666667, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI18390331, Length=150, Percent_Identity=36, Blast_Score=101, Evalue=3e-21,
Organism=Homo sapiens, GI53729339, Length=239, Percent_Identity=31.3807531380753, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI53729337, Length=239, Percent_Identity=31.3807531380753, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=41.4414414414414, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=41.4414414414414, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=41.4414414414414, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI217272894, Length=167, Percent_Identity=32.3353293413174, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI217272892, Length=167, Percent_Identity=32.3353293413174, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788922, Length=599, Percent_Identity=92.3205342237062, Blast_Score=1108, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=28.740157480315, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=35.4838709677419, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.6923076923077, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789559, Length=225, Percent_Identity=29.3333333333333, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789737, Length=331, Percent_Identity=25.6797583081571, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=331, Percent_Identity=25.6797583081571, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=41.2724306688418, Blast_Score=485, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=35.4838709677419, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=36.986301369863, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=228, Percent_Identity=29.8245614035088, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=37.037037037037, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=37.037037037037, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=34.4827586206897, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=234, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25141371, Length=240, Percent_Identity=29.5833333333333, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=45.9234608985025, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=36.8131868131868, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=40.9722222222222, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=40.9722222222222, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=37.3913043478261, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=39.3103448275862, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=243, Percent_Identity=28.3950617283951, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325337, Length=171, Percent_Identity=28.6549707602339, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319594, Length=171, Percent_Identity=28.6549707602339, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=42.9284525790349, Blast_Score=525, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.0248447204969, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=44.6043165467626, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=39.5973154362416, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585711, Length=162, Percent_Identity=34.5679012345679, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585713, Length=162, Percent_Identity=34.5679012345679, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585709, Length=162, Percent_Identity=34.5679012345679, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=34.8148148148148, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28572034, Length=245, Percent_Identity=29.7959183673469, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19921738, Length=245, Percent_Identity=27.7551020408163, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66710; Mature: 66710

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLSEREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYHSKDGQTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAIRCSAKTGVGVPDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PAPEGDPNGPLQALIIDSWFDNYLGVVSLIRIKNGSLRKGDKVKVMSTGQSYNADRLGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHEEECCCCCCCCCEEEEEECCCCCCCCEEECC
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLTRNPAEKSLPGFKKVKPQVYAGLF
CCHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCHHHCCCHHHEEEE
PISSDDYESFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCC
LELITTAPTVVYEVITTNQETVYVDSPSKLPALNNIEELREPIAECHMLLPQEYLGNVIT
EEEEECCHHHEEHEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LCIEKRGTQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
HHHHCCCCCCCEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINNERVDALALITHRDNAQYRGRDLVEKMKELIPRQQFDIAIQAAIGNHIIARS
EEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHCEEEEEHHCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDSK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLSEREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYHSKDGQTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAIRCSAKTGVGVPDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PAPEGDPNGPLQALIIDSWFDNYLGVVSLIRIKNGSLRKGDKVKVMSTGQSYNADRLGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHEEECCCCCCCCCEEEEEECCCCCCCCEEECC
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLTRNPAEKSLPGFKKVKPQVYAGLF
CCHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCHHHCCCHHHEEEE
PISSDDYESFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCC
LELITTAPTVVYEVITTNQETVYVDSPSKLPALNNIEELREPIAECHMLLPQEYLGNVIT
EEEEECCHHHEEHEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LCIEKRGTQTNMVYHGKQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
HHHHCCCCCCCEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINNERVDALALITHRDNAQYRGRDLVEKMKELIPRQQFDIAIQAAIGNHIIARS
EEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHCEEEEEHHCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDSK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA