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The map label for this gene is lacF [H]

Identifier: 218929565

GI number: 218929565

Start: 2777510

End: 2778385

Strand: Reverse

Name: lacF [H]

Synonym: YPO2476

Alternate gene names: 218929565

Gene position: 2778385-2777510 (Counterclockwise)

Preceding gene: 218929566

Following gene: 218929564

Centisome position: 59.7

GC content: 41.21

Gene sequence:

>876_bases
ATGACTTTTTTAAAGCTTAAAGATAAACAGGTAGGCAGCGTATTAGCGGCCTCTTATCTGGGATATACCAGCATATTCTG
GTTTTATCCTTTTATTTGGCTCGCCGTTTTATCGCTAACTGAGTGGCGTTTTGTTGGTATTCCTACGTTCAATGGTCTGA
ATAACTTTATTCTGGTAATGCAAGATCCTTTGTTTTGGAAATCGATGCTGAACGTGATGCGTTTCTTAATGTATTACTTG
CCCATTGTATTTATCTCTTCATTCCTGTTCGCGTTTGGTTTACAGAAATTAAAACATGGCAGGACCTTTGTTGCATTAAG
TTTTTTACTGGCTAACGTCTCATCTGGCGTGGCCTATTCCATCGTGTTCTCTAAGATATTTAGTCAGAATGGGCCGCTGA
ATACCTTCTTATATGATTGGTTTGGATTTACGCTGCCTTGGTTAACCAGCCCGGACTTCGCCATGTTATCTATCGCTTTG
GTCGTTACCTGGAAATTTGTCGGGTACTACGGGTTGATTCTCTTTTCTGGGTTAAACAGTATTCCCAAAGAAATTTATTC
AGCAGCGGAATTAGATAATACTCGCACCATTAAGCGCTTGTTCCATATCACATTACCGATGATCAACCCGCAATTAATTA
TGGTGCTGGTTTTAGCCATTACGGTCTCCTTCGCTATTTTTACCGAGCCATATCTCATTACTGGTGGCGGGCCGCTTAAT
AGCACCACCACGCCAATGATCGTGATGTATGAAACCGCGTTTATGAAGATGCAACCAAGTTGGGCAGCGACCATGTCAAT
CTTTATCGCGCTCATCAGTTTTGGAATTATCTTGGGTGTCAGAAAATTGCTGGAAAGAAAGGTAGAGATTGTTTAA

Upstream 100 bases:

>100_bases
AACGCTAGGATTTTAAAGTCCTAATATTTTGGAATCCTAGCAGTAAGAATGAGTTAATTTCTTGCTTTCTGCATCAAAGT
GAGCCAGGTGGCCTTTCATT

Downstream 100 bases:

>100_bases
ATGAAAAAAAACATCAACAGTTTAATTATCCATCTGGTTATGTTTTCTCTGGCGCTTTCGTGGCTCTATCCCTATATCTG
GATGGTGCTTTCTTCCTTTA

Product: sugar ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTFLKLKDKQVGSVLAASYLGYTSIFWFYPFIWLAVLSLTEWRFVGIPTFNGLNNFILVMQDPLFWKSMLNVMRFLMYYL
PIVFISSFLFAFGLQKLKHGRTFVALSFLLANVSSGVAYSIVFSKIFSQNGPLNTFLYDWFGFTLPWLTSPDFAMLSIAL
VVTWKFVGYYGLILFSGLNSIPKEIYSAAELDNTRTIKRLFHITLPMINPQLIMVLVLAITVSFAIFTEPYLITGGGPLN
STTTPMIVMYETAFMKMQPSWAATMSIFIALISFGIILGVRKLLERKVEIV

Sequences:

>Translated_291_residues
MTFLKLKDKQVGSVLAASYLGYTSIFWFYPFIWLAVLSLTEWRFVGIPTFNGLNNFILVMQDPLFWKSMLNVMRFLMYYL
PIVFISSFLFAFGLQKLKHGRTFVALSFLLANVSSGVAYSIVFSKIFSQNGPLNTFLYDWFGFTLPWLTSPDFAMLSIAL
VVTWKFVGYYGLILFSGLNSIPKEIYSAAELDNTRTIKRLFHITLPMINPQLIMVLVLAITVSFAIFTEPYLITGGGPLN
STTTPMIVMYETAFMKMQPSWAATMSIFIALISFGIILGVRKLLERKVEIV
>Mature_290_residues
TFLKLKDKQVGSVLAASYLGYTSIFWFYPFIWLAVLSLTEWRFVGIPTFNGLNNFILVMQDPLFWKSMLNVMRFLMYYLP
IVFISSFLFAFGLQKLKHGRTFVALSFLLANVSSGVAYSIVFSKIFSQNGPLNTFLYDWFGFTLPWLTSPDFAMLSIALV
VTWKFVGYYGLILFSGLNSIPKEIYSAAELDNTRTIKRLFHITLPMINPQLIMVLVLAITVSFAIFTEPYLITGGGPLNS
TTTPMIVMYETAFMKMQPSWAATMSIFIALISFGIILGVRKLLERKVEIV

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787570, Length=242, Percent_Identity=25.6198347107438, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1789861, Length=287, Percent_Identity=24.0418118466899, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33090; Mature: 32959

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLKLKDKQVGSVLAASYLGYTSIFWFYPFIWLAVLSLTEWRFVGIPTFNGLNNFILVM
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEE
QDPLFWKSMLNVMRFLMYYLPIVFISSFLFAFGLQKLKHGRTFVALSFLLANVSSGVAYS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
IVFSKIFSQNGPLNTFLYDWFGFTLPWLTSPDFAMLSIALVVTWKFVGYYGLILFSGLNS
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPKEIYSAAELDNTRTIKRLFHITLPMINPQLIMVLVLAITVSFAIFTEPYLITGGGPLN
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
STTTPMIVMYETAFMKMQPSWAATMSIFIALISFGIILGVRKLLERKVEIV
CCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFLKLKDKQVGSVLAASYLGYTSIFWFYPFIWLAVLSLTEWRFVGIPTFNGLNNFILVM
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEE
QDPLFWKSMLNVMRFLMYYLPIVFISSFLFAFGLQKLKHGRTFVALSFLLANVSSGVAYS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHH
IVFSKIFSQNGPLNTFLYDWFGFTLPWLTSPDFAMLSIALVVTWKFVGYYGLILFSGLNS
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPKEIYSAAELDNTRTIKRLFHITLPMINPQLIMVLVLAITVSFAIFTEPYLITGGGPLN
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
STTTPMIVMYETAFMKMQPSWAATMSIFIALISFGIILGVRKLLERKVEIV
CCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]