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The map label for this gene is modE

Identifier: 218928307

GI number: 218928307

Start: 1289307

End: 1290098

Strand: Reverse

Name: modE

Synonym: YPO1143

Alternate gene names: 218928307

Gene position: 1290098-1289307 (Counterclockwise)

Preceding gene: 218928312

Following gene: 218928306

Centisome position: 27.72

GC content: 51.01

Gene sequence:

>792_bases
ATGCAGGCCGAAATTCTTCTTACACTAAAACTTCAACAGCGCCTGTTTGCCGATCCACGCCGTATTGCACTGTTAAAGCA
AATTCAGCATACCGGTTCCATCAGCCAGGGAGCCAAGCTCGCGGGTATCAGCTATAAAAGTGCCTGGGATGCCATAAATG
ACATGAATACACTGTCCGAGGAGATACTGGTTGAACGGGCCACTGGCGGCAAAGGTGGAGGGGGTGCACATCTGACCCGC
TACGGTGAACGCTTAATCCAGCTTTATGATTTACTCGCAACCATCCAGCAAAAAGCCTTTGATACCCTGAAAGATGATTC
ACTGCCGCTGGACAGCTTGCTGGCGGCAATTTCCCGTTTTTCACTGCAAACCAGCGCCCGTAATCAATTTTTCGGTACCC
TTATTGAACGCGACCACCAGCAGGTGCAACAGCATGTCAATATTCTGCTATCGGACGGTAAAACTCGCTTAACGGCGGCG
ATCACCCAACAAAGCGCGGATCGCCTGCAACTTTCAGCAGGTAAAGAAGTGCTGGCATTGATTAAAGCACCTTGGGTAAA
ACTGGTTACCGATCCGGCACTGGCAGGTGCAGCGGATAACGCATTGCCGGGAACGGTCGCCAGCATTGAGCCGGGCAACG
ATCATAGCGAAGTTATTGTCACCTTAACTGGCGGGGCCAACCTGTGTTCAACCCAAAACAACAGCGAACTCCATGCGCTG
AATTTACGGGTTGGCAGTGCCGTCATTGCACAATTCAATGCAGATCGGGTGATTATCGCCACATTGTGTTGA

Upstream 100 bases:

>100_bases
TTGTTTAGCTATCTATGTTGTTTAGCTATCTATGTTGTTTAGCTATCTATGCCATTTAGCTATCTATGACAATAACTGTT
TCCCGCCGGAGCAAAAAACA

Downstream 100 bases:

>100_bases
ATGATTATTCCCAGTAATCACTGTTGGACCATACATTTGTTCGTTGACATTTTTCGTCTTTCCGCTTATCCCTAACATCT
TTACTACGTTGCAAAATATG

Product: DNA-binding transcriptional regulator ModE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MQAEILLTLKLQQRLFADPRRIALLKQIQHTGSISQGAKLAGISYKSAWDAINDMNTLSEEILVERATGGKGGGGAHLTR
YGERLIQLYDLLATIQQKAFDTLKDDSLPLDSLLAAISRFSLQTSARNQFFGTLIERDHQQVQQHVNILLSDGKTRLTAA
ITQQSADRLQLSAGKEVLALIKAPWVKLVTDPALAGAADNALPGTVASIEPGNDHSEVIVTLTGGANLCSTQNNSELHAL
NLRVGSAVIAQFNADRVIIATLC

Sequences:

>Translated_263_residues
MQAEILLTLKLQQRLFADPRRIALLKQIQHTGSISQGAKLAGISYKSAWDAINDMNTLSEEILVERATGGKGGGGAHLTR
YGERLIQLYDLLATIQQKAFDTLKDDSLPLDSLLAAISRFSLQTSARNQFFGTLIERDHQQVQQHVNILLSDGKTRLTAA
ITQQSADRLQLSAGKEVLALIKAPWVKLVTDPALAGAADNALPGTVASIEPGNDHSEVIVTLTGGANLCSTQNNSELHAL
NLRVGSAVIAQFNADRVIIATLC
>Mature_263_residues
MQAEILLTLKLQQRLFADPRRIALLKQIQHTGSISQGAKLAGISYKSAWDAINDMNTLSEEILVERATGGKGGGGAHLTR
YGERLIQLYDLLATIQQKAFDTLKDDSLPLDSLLAAISRFSLQTSARNQFFGTLIERDHQQVQQHVNILLSDGKTRLTAA
ITQQSADRLQLSAGKEVLALIKAPWVKLVTDPALAGAADNALPGTVASIEPGNDHSEVIVTLTGGANLCSTQNNSELHAL
NLRVGSAVIAQFNADRVIIATLC

Specific function: The modE-Mo complex acts as a repressor of the modABC operon, involved in the transport of molybdate. Upon binding molybdate, the conformation of the protein changes, promoting dimerization of ModE-Mo. The protein dimer is then competent to bind a DNA reg

COG id: COG2005

COG function: function code R; N-terminal domain of molybdenum-binding protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the modE family

Homologues:

Organism=Escherichia coli, GI1786976, Length=264, Percent_Identity=70.4545454545455, Blast_Score=315, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MODE_YERPE (P58497)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AE0140
- RefSeq:   NP_670339.1
- RefSeq:   NP_992391.1
- RefSeq:   YP_002346182.1
- ProteinModelPortal:   P58497
- SMR:   P58497
- IntAct:   P58497
- GeneID:   1147986
- GeneID:   1173992
- GeneID:   2764726
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1143
- KEGG:   ypk:y3040
- KEGG:   ypm:YP_1017
- HOGENOM:   HBG405634
- OMA:   AINEMNT
- ProtClustDB:   PRK10676
- BioCyc:   YPES187410:Y3040-MONOMER
- BioCyc:   YPES214092:YPO1143-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR004606
- InterPro:   IPR008995
- InterPro:   IPR003725
- InterPro:   IPR016462
- InterPro:   IPR005116
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PIRSF:   PIRSF005763
- TIGRFAMs:   TIGR00637
- TIGRFAMs:   TIGR00638

Pfam domain/function: PF00126 HTH_1; PF03459 TOBE; SSF50331 MOP_like

EC number: NA

Molecular weight: Translated: 28299; Mature: 28299

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAEILLTLKLQQRLFADPRRIALLKQIQHTGSISQGAKLAGISYKSAWDAINDMNTLSE
CCCEEEEEEEHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
EILVERATGGKGGGGAHLTRYGERLIQLYDLLATIQQKAFDTLKDDSLPLDSLLAAISRF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SLQTSARNQFFGTLIERDHQQVQQHVNILLSDGKTRLTAAITQQSADRLQLSAGKEVLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCHHHHHHHCCHHHHHH
IKAPWVKLVTDPALAGAADNALPGTVASIEPGNDHSEVIVTLTGGANLCSTQNNSELHAL
HHCCHHHEECCCHHCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCCEEEE
NLRVGSAVIAQFNADRVIIATLC
EEEHHHHHHEEECCCEEEEEEEC
>Mature Secondary Structure
MQAEILLTLKLQQRLFADPRRIALLKQIQHTGSISQGAKLAGISYKSAWDAINDMNTLSE
CCCEEEEEEEHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
EILVERATGGKGGGGAHLTRYGERLIQLYDLLATIQQKAFDTLKDDSLPLDSLLAAISRF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SLQTSARNQFFGTLIERDHQQVQQHVNILLSDGKTRLTAAITQQSADRLQLSAGKEVLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCHHHHHHHCCHHHHHH
IKAPWVKLVTDPALAGAADNALPGTVASIEPGNDHSEVIVTLTGGANLCSTQNNSELHAL
HHCCHHHEECCCHHCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCCEEEE
NLRVGSAVIAQFNADRVIIATLC
EEEHHHHHHEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430