BacMap

Definition	Yersinia pestis CO92 chromosome, complete genome.
Accession	NC_003143
Length	4,653,728

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The map label for this gene is dkgB

Identifier: 218928244

GI number: 218928244

Start: 1223177

End: 1223980

Strand: Direct

Name: dkgB

Synonym: YPO1075

Alternate gene names: 218928244

Gene position: 1223177-1223980 (Clockwise)

Preceding gene: 218928243

Following gene: 218928245

Centisome position: 26.28

GC content: 40.8

Gene sequence:

>804_bases
ATGAGCATACCTGTATTCGGATTAGGAACTTTTCGCCTTCAAGATCAAATTGTTATTGATTCAGTGAGCCAAGCTCTGAC
GTTGGGCTATCGGGCGATTGATACAGCACAAATATATGAAAATGAAGCGCCTGTTGGTCAGGCAATCCAAGAAAGTGGTA
TTAATCGTGATGAACTATTTATTACTACTAAGATCTGGATTGCTAATTTATCAAAAGACAAATTAATTCCCAGCCTGCGA
GAAAGCATACAGAAACTGAAAACTGACTATGTAGATTTAACACTGATTCATTGGCCATCACCTAATGATGAAGTTTCTGT
TGCTGAATTTATGTCCGAGTTATTAAAAGCAAAAGGGCTAGGCTTAACTCGTCAAATTGGTATTTCTAACTTTACTATTG
ATTTAATGAAGCAGGCGATTGCTGCCGTTGGTGCAGAAGAGATTGCAACTAATCAAATCGAGCTTTCTCCATTATTACAA
AACCGTAATGTTGTTGATTTTGCCAAACAAAATGGCATTGCAGTGACTTCCTATATGACACTGGCTTATGGCAAGGCACT
TGCAGAGCCAGTGATTAAAACGATTGCTGAGCAACATGGTGCGACCCCCGCACAAGTTATTTTGAGTTGGGCGATGCAAT
TGGGTTATGGCGTTATTCCGTCATCGACTAAAGCTGCTAACTTGGCCAGCAACTTACTAGCACAGAAATTATGCCTGAAT
GCAGCAGATATGGCGTTGATCGCCACATTGGATCGCAATGAGAGGTTGGTCAGCCCTGATGGTTTAGCACCTAAATGGGA
TTAA

Upstream 100 bases:

>100_bases
AGTTTTTCATCTGGTAGTTATTTAGAACATTCCACTGCCGATAAAAATTCGTTATGACTCGCAAGATAAAATGATTGTTA
GCAAAAAATGAGGAACTATT

Downstream 100 bases:

>100_bases
TAGCAGCCTAAGACACTGGACGAGCCACCTTACATTGAGGTGGTTTTTTATTTTGGTGCATCCTGACGGGGCTGAAACAG
GCTCGGTGAAAAGTGTGCGG

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAPVGQAIQESGINRDELFITTKIWIANLSKDKLIPSLR
ESIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGLGLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQ
NRNVVDFAKQNGIAVTSYMTLAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLN
AADMALIATLDRNERLVSPDGLAPKWD

Sequences:

>Translated_267_residues
MSIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAPVGQAIQESGINRDELFITTKIWIANLSKDKLIPSLR
ESIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGLGLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQ
NRNVVDFAKQNGIAVTSYMTLAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLN
AADMALIATLDRNERLVSPDGLAPKWD
>Mature_266_residues
SIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAPVGQAIQESGINRDELFITTKIWIANLSKDKLIPSLRE
SIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGLGLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQN
RNVVDFAKQNGIAVTSYMTLAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLNA
ADMALIATLDRNERLVSPDGLAPKWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG)

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family

Homologues:

Organism=Homo sapiens, GI93277124, Length=300, Percent_Identity=30.6666666666667, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI223468663, Length=289, Percent_Identity=31.1418685121107, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=30, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=29.0657439446367, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=29.0657439446367, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI310109920, Length=297, Percent_Identity=28.6195286195286, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI45446745, Length=297, Percent_Identity=28.6195286195286, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI4503285, Length=297, Percent_Identity=28.6195286195286, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI5453543, Length=297, Percent_Identity=28.2828282828283, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI24497585, Length=296, Percent_Identity=28.3783783783784, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=28.956228956229, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI4502049, Length=291, Percent_Identity=32.9896907216495, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI291291012, Length=273, Percent_Identity=30.4029304029304, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=27.6450511945392, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI300116273, Length=268, Percent_Identity=27.6119402985075, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=27.3381294964029, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=35.0877192982456, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=35.0877192982456, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI310109924, Length=160, Percent_Identity=28.125, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=75.2808988764045, Blast_Score=403, Evalue=1e-114,
Organism=Escherichia coli, GI87082198, Length=262, Percent_Identity=37.4045801526718, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1788081, Length=263, Percent_Identity=26.9961977186312, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI48994888, Length=250, Percent_Identity=25.6, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787674, Length=273, Percent_Identity=27.8388278388278, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788070, Length=216, Percent_Identity=26.8518518518519, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17550248, Length=290, Percent_Identity=33.448275862069, Blast_Score=148, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=31.9444444444444, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=32.0689655172414, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17561298, Length=275, Percent_Identity=28.3636363636364, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17552492, Length=268, Percent_Identity=30.2238805970149, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17566692, Length=288, Percent_Identity=30.5555555555556, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17561300, Length=274, Percent_Identity=29.1970802919708, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71998625, Length=265, Percent_Identity=29.0566037735849, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17537079, Length=295, Percent_Identity=27.1186440677966, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17564128, Length=297, Percent_Identity=28.2828282828283, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17562292, Length=269, Percent_Identity=29.368029739777, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17538386, Length=268, Percent_Identity=29.4776119402985, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324694, Length=292, Percent_Identity=34.5890410958904, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=36.8794326241135, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321896, Length=315, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6322556, Length=262, Percent_Identity=26.7175572519084, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6319625, Length=269, Percent_Identity=31.2267657992565, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320079, Length=242, Percent_Identity=29.3388429752066, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24644950, Length=298, Percent_Identity=33.2214765100671, Blast_Score=151, Evalue=4e-37,
Organism=Drosophila melanogaster, GI221378297, Length=315, Percent_Identity=31.1111111111111, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24663317, Length=289, Percent_Identity=30.1038062283737, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=31.9727891156463, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24657054, Length=280, Percent_Identity=30.7142857142857, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=31.1188811188811, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21356425, Length=281, Percent_Identity=29.1814946619217, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45553081, Length=284, Percent_Identity=27.112676056338, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24662785, Length=290, Percent_Identity=32.4137931034483, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24662781, Length=290, Percent_Identity=31.3793103448276, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=26.3565891472868, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DKGB_YERPE (Q8ZH36)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AC0132
- RefSeq:   NP_670400.1
- RefSeq:   NP_994081.1
- RefSeq:   YP_002346119.1
- ProteinModelPortal:   Q8ZH36
- SMR:   Q8ZH36
- GeneID:   1148048
- GeneID:   1173921
- GeneID:   2764883
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1075
- KEGG:   ypk:y3101
- KEGG:   ypm:YP_2774
- HOGENOM:   HBG605727
- OMA:   LAWAMQL
- ProtClustDB:   PRK11172
- BioCyc:   YPES187410:Y3101-MONOMER
- BioCyc:   YPES214092:YPO1075-MONOMER
- BRENDA:   1.1.1.274
- GO:   GO:0005737
- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210
- Gene3D:   G3DSA:3.20.20.100
- PANTHER:   PTHR11732
- PIRSF:   PIRSF000097
- PRINTS:   PR00069

Pfam domain/function: PF00248 Aldo_ket_red; SSF51430 Aldo/ket_red

EC number: =1.1.1.274

Molecular weight: Translated: 29036; Mature: 28905

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1; PS00062 ALDOKETO_REDUCTASE_2; PS00063 ALDOKETO_REDUCTASE_3

Important sites: ACT_SITE 39-39 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAPVGQAIQESGINRDELF
CCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHEE
ITTKIWIANLSKDKLIPSLRESIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGL
EEEEEEEEECCHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCC
GLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQNRNVVDFAKQNGIAVTSYMT
CCEEECCCCHHHHHHHHHHHHHHCHHHHHCCCEEEHHHHCCCCCHHHHHCCCEEEHHHHH
LAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
AADMALIATLDRNERLVSPDGLAPKWD
HHHHHEEEEECCCCEEECCCCCCCCCC
>Mature Secondary Structure 
SIPVFGLGTFRLQDQIVIDSVSQALTLGYRAIDTAQIYENEAPVGQAIQESGINRDELF
CCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHEE
ITTKIWIANLSKDKLIPSLRESIQKLKTDYVDLTLIHWPSPNDEVSVAEFMSELLKAKGL
EEEEEEEEECCHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCC
GLTRQIGISNFTIDLMKQAIAAVGAEEIATNQIELSPLLQNRNVVDFAKQNGIAVTSYMT
CCEEECCCCHHHHHHHHHHHHHHCHHHHHCCCEEEHHHHCCCCCHHHHHCCCEEEHHHHH
LAYGKALAEPVIKTIAEQHGATPAQVILSWAMQLGYGVIPSSTKAANLASNLLAQKLCLN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
AADMALIATLDRNERLVSPDGLAPKWD
HHHHHEEEEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430