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The map label for this gene is ampM [H]

Identifier: 218928211

GI number: 218928211

Start: 1184138

End: 1184929

Strand: Reverse

Name: ampM [H]

Synonym: YPO1043

Alternate gene names: 218928211

Gene position: 1184929-1184138 (Counterclockwise)

Preceding gene: 218928232

Following gene: 218928210

Centisome position: 25.46

GC content: 48.86

Gene sequence:

>792_bases
ATGGCAATCTCAATCAAAACACCTGAAGATATTCAAAAAATGCGCGTTGCTGGCCGCTTGGCTGCTGAAGTGCTGGAAAT
CATCGAACCCTATGTGAAACCAGGGGTAACAACGGGTGAACTGGATCGTATCTGTCACGATCATATCACCAACCACCAAC
AGGCTATTTCTGCCTGCCTGGGGTATCACGGTTTCCCTAAATCGGTCTGCATCTCGGTAAATGAAGTGATCTGCCACGGC
ATCCCAAGCGATGAAAAAGTGCTGAAAGAGGGTGATATCGTCAATATCGACGTGACCGTGATTAAAGATGGCTTCCATGG
CGATACCTCAAAAATGTTTATTGTTGGTAAACCGACCATTCTGGGTGAACGTCTGTGCCGCGTGACTCAAGAGAGCCTGT
ACCTGGCGATTAAAATGGTTAAACCGGGGATCCGCCTGCGGACGCTGGGTAAAGCTATCCAGAAATTTGTTGAAGCAGAG
AACTTCTCCGTAGTACGTGAATATTGTGGTCACGGGATCGGTGAAGGTTTCCATGAAGAGCCACAAGTTTTACATTACGA
TGCGGATGACGGCGGCGTCGTCCTGCAAGCAGGTATGGCCTTCACGATCGAGCCCATGGTGAACGCCGGGGATTACCGGA
TTCGGACCATGAAAGACGGCTGGACGGTAAAAACTAAAGATCGCAACTTGTCGGCGCAGTATGAGCATACTATTGTGGTA
ACCGATAACGGCTGCGAAATAATGACGTTGCGTAAGGATGACACTATCCCCAACATCATAACGCACGAGTAA

Upstream 100 bases:

>100_bases
ATTAGCCACAGATTTTTATTAATCGACAGTTATCTTTTCATTATCTGCATCTCATTAATAACGGCGGCTGGCTATTTACA
GCCCCTACGGACACTTTTGC

Downstream 100 bases:

>100_bases
TAATGACAAGCCGGCACCGCCGGCTTTTTTATGGGCTGCGTTCCTCATCGGGTAAACATATGTCTGACAATCACACCGAA
CACTCCCTATCGCTGACGCT

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MAISIKTPEDIQKMRVAGRLAAEVLEIIEPYVKPGVTTGELDRICHDHITNHQQAISACLGYHGFPKSVCISVNEVICHG
IPSDEKVLKEGDIVNIDVTVIKDGFHGDTSKMFIVGKPTILGERLCRVTQESLYLAIKMVKPGIRLRTLGKAIQKFVEAE
NFSVVREYCGHGIGEGFHEEPQVLHYDADDGGVVLQAGMAFTIEPMVNAGDYRIRTMKDGWTVKTKDRNLSAQYEHTIVV
TDNGCEIMTLRKDDTIPNIITHE

Sequences:

>Translated_263_residues
MAISIKTPEDIQKMRVAGRLAAEVLEIIEPYVKPGVTTGELDRICHDHITNHQQAISACLGYHGFPKSVCISVNEVICHG
IPSDEKVLKEGDIVNIDVTVIKDGFHGDTSKMFIVGKPTILGERLCRVTQESLYLAIKMVKPGIRLRTLGKAIQKFVEAE
NFSVVREYCGHGIGEGFHEEPQVLHYDADDGGVVLQAGMAFTIEPMVNAGDYRIRTMKDGWTVKTKDRNLSAQYEHTIVV
TDNGCEIMTLRKDDTIPNIITHE
>Mature_262_residues
AISIKTPEDIQKMRVAGRLAAEVLEIIEPYVKPGVTTGELDRICHDHITNHQQAISACLGYHGFPKSVCISVNEVICHGI
PSDEKVLKEGDIVNIDVTVIKDGFHGDTSKMFIVGKPTILGERLCRVTQESLYLAIKMVKPGIRLRTLGKAIQKFVEAEN
FSVVREYCGHGIGEGFHEEPQVLHYDADDGGVVLQAGMAFTIEPMVNAGDYRIRTMKDGWTVKTKDRNLSAQYEHTIVVT
DNGCEIMTLRKDDTIPNIITHE

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=260, Percent_Identity=47.6923076923077, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI40385867, Length=248, Percent_Identity=41.1290322580645, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI1786364, Length=263, Percent_Identity=84.0304182509506, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1788728, Length=207, Percent_Identity=25.6038647342995, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71996291, Length=250, Percent_Identity=47.6, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6323273, Length=251, Percent_Identity=48.207171314741, Blast_Score=228, Evalue=7e-61,
Organism=Drosophila melanogaster, GI21355531, Length=253, Percent_Identity=46.2450592885375, Blast_Score=226, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24583427, Length=245, Percent_Identity=41.2244897959184, Blast_Score=195, Evalue=2e-50,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 29201; Mature: 29070

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISIKTPEDIQKMRVAGRLAAEVLEIIEPYVKPGVTTGELDRICHDHITNHQQAISACL
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHH
GYHGFPKSVCISVNEVICHGIPSDEKVLKEGDIVNIDVTVIKDGFHGDTSKMFIVGKPTI
CCCCCCHHHHEEHHHHHCCCCCCCHHHHHCCCEEEEEEEEEECCCCCCCCEEEEEECCHH
LGERLCRVTQESLYLAIKMVKPGIRLRTLGKAIQKFVEAENFSVVREYCGHGIGEGFHEE
HHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
PQVLHYDADDGGVVLQAGMAFTIEPMVNAGDYRIRTMKDGWTVKTKDRNLSAQYEHTIVV
CCEEEEECCCCCEEEECCCEEEEEECCCCCCEEEEEECCCCEEEECCCCCCCEEEEEEEE
TDNGCEIMTLRKDDTIPNIITHE
ECCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AISIKTPEDIQKMRVAGRLAAEVLEIIEPYVKPGVTTGELDRICHDHITNHQQAISACL
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHH
GYHGFPKSVCISVNEVICHGIPSDEKVLKEGDIVNIDVTVIKDGFHGDTSKMFIVGKPTI
CCCCCCHHHHEEHHHHHCCCCCCCHHHHHCCCEEEEEEEEEECCCCCCCCEEEEEECCHH
LGERLCRVTQESLYLAIKMVKPGIRLRTLGKAIQKFVEAENFSVVREYCGHGIGEGFHEE
HHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
PQVLHYDADDGGVVLQAGMAFTIEPMVNAGDYRIRTMKDGWTVKTKDRNLSAQYEHTIVV
CCEEEEECCCCCEEEECCCEEEEEECCCCCCEEEEEECCCCEEEECCCCCCCEEEEEEEE
TDNGCEIMTLRKDDTIPNIITHE
ECCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]