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The map label for this gene is amiC [H]

Identifier: 218928192

GI number: 218928192

Start: 1163330

End: 1164580

Strand: Direct

Name: amiC [H]

Synonym: YPO1023

Alternate gene names: 218928192

Gene position: 1163330-1164580 (Clockwise)

Preceding gene: 218928190

Following gene: 218928193

Centisome position: 25.0

GC content: 46.6

Gene sequence:

>1251_bases
ATGGCAGATTCAAATCATAATTCTGGTCGTCGCCGTCTATTACAAGGTGCCGCAGCAGCTTGGATGCTGAGTATAAGCCG
GGTTGGATTTGCCGCATCTTCACATATTGTTGCTGTCCGGGTGTGGCCCTCTTCTACTTACACCCGAGTCACATTAGAGT
CAAATACACCACTGAAGTACCGCCAATTCGCCTTAACTCATCCCGACCGTATTGTTGTTGATATTGAAGGCGTACAGCTA
AATAGCGTATTGAAAGAGATCAGCCGGCAAGTTCAATCCGCTGACCCTTATCTCAAACAGGCGCGGGTAGGGCAGTTCGA
TAAAAATACTGTTCGTTTGGTATTGGAACTGAAGCAGAGTATCAGCCCTCAATTATTTACCCTGAAACCTTTCGCTGAAT
TCCGTAATCGCTTAGTGGTCGATCTGTACCCTGCGGAGGGGGGCACATCGGCAGAAGATGATCCGCTGCTGGCCCTATTG
GAAGATTACAATAAAGGTAATGTGGATCGAACCTTGCCACCAGAAACACCCAAAGCAGGTAAAGCAGGGCGTGACAGACC
CATTATTATCATGTTGGACCCAGGACATGGCGGGGAAGATCCTGGGGCTATCGGCAAAAATAAAACCAAGGAAAAAGACA
TTGTGCTGCAAATTGCTCGTCGGTTACGGGCGCTTATTCAGAAAGAAGCCAATATGCGGGTGTTTATGACTCGTAATGAA
GATGTCTTTATCCCGCTGAAAGTTCGGGTGGCAAAAGCACGTAAGCTTCGTGCGGATTTATTCATTTCGATTCATGCCGA
TGCTTTTACCAATCGGGCGGCTAGCGGCTCCTCGGTCTTTGCTTTATCGACCAAAGGAGCCACCAGTACTGCCGCTCGTT
TTCTGGCTCAGACACAAAATGAGGCCGATCAGATTGGTGGGGTCAGCAAAAGTGGTGATCCTTATCTTGACCATACCATT
ATTGATTTATTGCAAACCGCAACTATCAACGATAGCTTGAAGTTTGGTAAAGAAGTACTGAGTCGGATGAGTAAAATCAA
CAAATTGCACAAAAATCGTGTCGATCAAGCAGGATTTGCGGTACTAAAAGCACCTGATATCCCGTCAATTCTGGTAGAAA
CTGCGTTCATCAGTAATTTGGAAGAAGAGCGGAAATTACGTACCAGCCGTTTTCAGCAACAGATAGCGGAATCTATTTTT
GCTGGTATCAAAGCGTACTTTGCTAATGGCGGGGCAATGGCGCGATTGTGA

Upstream 100 bases:

>100_bases
GTTTTTCTGATGACAGCGTTAAATTGATTCGTTAAAGTTTTTCCTCATAATTTTTTTGGTTTGTTTTTTCATTAGATTTT
AGTTGTTACTTTGGAGTTAC

Downstream 100 bases:

>100_bases
TGGATGTTTCTGTAAAATAATGGGTGTTTCTGTCAAAAATAGAGTGACTATAAATAGCGTGGTTATAGTTCAATACACGA
GGACGAGGCATCCTACCAGG

Product: N-acetylmuramoyl-L-alanine amidase AmiC precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLESNTPLKYRQFALTHPDRIVVDIEGVQL
NSVLKEISRQVQSADPYLKQARVGQFDKNTVRLVLELKQSISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDDPLLALL
EDYNKGNVDRTLPPETPKAGKAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRVFMTRNE
DVFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARFLAQTQNEADQIGGVSKSGDPYLDHTI
IDLLQTATINDSLKFGKEVLSRMSKINKLHKNRVDQAGFAVLKAPDIPSILVETAFISNLEEERKLRTSRFQQQIAESIF
AGIKAYFANGGAMARL

Sequences:

>Translated_416_residues
MADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLESNTPLKYRQFALTHPDRIVVDIEGVQL
NSVLKEISRQVQSADPYLKQARVGQFDKNTVRLVLELKQSISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDDPLLALL
EDYNKGNVDRTLPPETPKAGKAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRVFMTRNE
DVFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARFLAQTQNEADQIGGVSKSGDPYLDHTI
IDLLQTATINDSLKFGKEVLSRMSKINKLHKNRVDQAGFAVLKAPDIPSILVETAFISNLEEERKLRTSRFQQQIAESIF
AGIKAYFANGGAMARL
>Mature_415_residues
ADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLESNTPLKYRQFALTHPDRIVVDIEGVQLN
SVLKEISRQVQSADPYLKQARVGQFDKNTVRLVLELKQSISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDDPLLALLE
DYNKGNVDRTLPPETPKAGKAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRVFMTRNED
VFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARFLAQTQNEADQIGGVSKSGDPYLDHTII
DLLQTATINDSLKFGKEVLSRMSKINKLHKNRVDQAGFAVLKAPDIPSILVETAFISNLEEERKLRTSRFQQQIAESIFA
GIKAYFANGGAMARL

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=419, Percent_Identity=70.4057279236277, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1788776, Length=232, Percent_Identity=48.2758620689655, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=41.7777777777778, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45933; Mature: 45802

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLESNTPLKY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCE
RQFALTHPDRIVVDIEGVQLNSVLKEISRQVQSADPYLKQARVGQFDKNTVRLVLELKQS
EEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHH
ISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDDPLLALLEDYNKGNVDRTLPPETPKAG
CCCCEEEECHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
KAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRVFMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
DVFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARFLAQTQN
CEEEEEEEEEHHHHHHEEEEEEEEECHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCC
EADQIGGVSKSGDPYLDHTIIDLLQTATINDSLKFGKEVLSRMSKINKLHKNRVDQAGFA
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEE
VLKAPDIPSILVETAFISNLEEERKLRTSRFQQQIAESIFAGIKAYFANGGAMARL
EEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADSNHNSGRRRLLQGAAAAWMLSISRVGFAASSHIVAVRVWPSSTYTRVTLESNTPLKY
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCE
RQFALTHPDRIVVDIEGVQLNSVLKEISRQVQSADPYLKQARVGQFDKNTVRLVLELKQS
EEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHH
ISPQLFTLKPFAEFRNRLVVDLYPAEGGTSAEDDPLLALLEDYNKGNVDRTLPPETPKAG
CCCCEEEECHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
KAGRDRPIIIMLDPGHGGEDPGAIGKNKTKEKDIVLQIARRLRALIQKEANMRVFMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
DVFIPLKVRVAKARKLRADLFISIHADAFTNRAASGSSVFALSTKGATSTAARFLAQTQN
CEEEEEEEEEHHHHHHEEEEEEEEECHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCC
EADQIGGVSKSGDPYLDHTIIDLLQTATINDSLKFGKEVLSRMSKINKLHKNRVDQAGFA
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEE
VLKAPDIPSILVETAFISNLEEERKLRTSRFQQQIAESIFAGIKAYFANGGAMARL
EEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]