Click here to switch to the map view.

The map label for this gene is insA [H]

Identifier: 218928153

GI number: 218928153

Start: 1088356

End: 1088619

Strand: Reverse

Name: insA [H]

Synonym: YPO0981

Alternate gene names: 218928153

Gene position: 1088619-1088356 (Counterclockwise)

Preceding gene: 218928154

Following gene: 218928151

Centisome position: 23.39

GC content: 47.73

Gene sequence:

>264_bases
ATGGCTTGCATTAACGTTCATTGTCCCCGTTGTGATTCTGCTTTGGTTTATCGACATGGTCAGAACCCTAAGGGCCAAAG
CCGTTTCCGCTGCCGTGAGTGCCGCTCTGTATTCCAACTGACCTACACTTATCAGGCCAGAAAGCCCGAAGTTAAAGGGC
AGATCGTTGATATAGCCTTTAGCGGCACAGGTGTGTGGGATACGGCAAGAACGCTAAAAATCGGTATCAATACCGTTATC
CGCACTTTAAAAAACTCTTGCTGA

Upstream 100 bases:

>100_bases
GGTAAAGAATATAAATTAAAAATAGCCGAATAATATTAATTTCGGTAATGACTCCAATTGGTTGAATTTGATGTACAATG
CGATTTTTTGAGGTTTGCTC

Downstream 100 bases:

>100_bases
AGCGAATAACGTCCTCGCCAGTCGCTCATGTAGACGTGGCGCTCATCTGTGAACTCGATGAGCAGTGGAGTTTTGTCGGC
AACAAGGCCCATCAGTAGAA

Product: insertion element protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 87; Mature: 86

Protein sequence:

>87_residues
MACINVHCPRCDSALVYRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPEVKGQIVDIAFSGTGVWDTARTLKIGINTVI
RTLKNSC

Sequences:

>Translated_87_residues
MACINVHCPRCDSALVYRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPEVKGQIVDIAFSGTGVWDTARTLKIGINTVI
RTLKNSC
>Mature_86_residues
ACINVHCPRCDSALVYRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPEVKGQIVDIAFSGTGVWDTARTLKIGINTVIR
TLKNSC

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=87, Percent_Identity=40.2298850574713, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1788204, Length=87, Percent_Identity=40.2298850574713, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1786204, Length=87, Percent_Identity=40.2298850574713, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI87081814, Length=87, Percent_Identity=40.2298850574713, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1786471, Length=87, Percent_Identity=40.2298850574713, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1786460, Length=87, Percent_Identity=40.2298850574713, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1790747, Length=87, Percent_Identity=41.3793103448276, Blast_Score=77, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 9865; Mature: 9733

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
7.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACINVHCPRCDSALVYRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPEVKGQIVDIAF
CCEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEE
SGTGVWDTARTLKIGINTVIRTLKNSC
CCCCCCCCCHHEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
ACINVHCPRCDSALVYRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPEVKGQIVDIAF
CEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEE
SGTGVWDTARTLKIGINTVIRTLKNSC
CCCCCCCCCHHEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]