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The map label for this gene is cycB [H]

Identifier: 218928032

GI number: 218928032

Start: 941812

End: 943074

Strand: Reverse

Name: cycB [H]

Synonym: YPO0856

Alternate gene names: 218928032

Gene position: 943074-941812 (Counterclockwise)

Preceding gene: 218928041

Following gene: 218928031

Centisome position: 20.26

GC content: 47.43

Gene sequence:

>1263_bases
ATGTTATACCTTCACCCGAGGCTAATAATGAAAATCAATAAAATCACTGCGCTTGTTTTAACCGCACTGGCAGTCACCCA
GTTTGCTGGTTTCGCTGCCCACGCTGCTACACAGCAATTAACAGTTTGGGAAGACATCAAAAAATCTGCCGGTATTAAAG
AGGCTATCGCTGATTTTGAAAAACAGCATCAGGTTAAGGTCAATGTGCTGGAAATGCCTTACGCACAACAAATTGAAAAA
CTCCGCCTTGATGGCCCTGCAGGTATCGGCCCTGATGTGTTGGTGATTCCCAATGATCAGTTAGGTGGTGCGGTAGTGCA
AGGTTTGCTGACACCGCTCAGCGTTGATCCAACCATAGTCACTACTTTTACTAAACCTTCTATCGCTGCCTTCACCATGG
ATAATGCCCTCTACGGTTTACCGAAAGCCGTGGAAACGCTGGTGATGATCTACAACAAAGACATGCTGCCAACGCCGTTA
GCTACCTTGGATGAGTACGCCGCATTCTCTAAGAAACAACGCGCAGAAAATAAATATGGTCTGTTGGCGAAGTTCGATCA
GATCTATTACAGCTGGGGAGCGATCGAGCCAATGGGCGGTTACATCTTTGGTAAAGATGCTAACGGTAGCTTGAAGGCTA
ACGATATCGGGCTAAATACACCAGGGGCTGTTGAGGCCGTAACCTATTTGAAAACATTCTATGCTAACGGTCTGTTTCCA
ATTGGCACCATCGGTGATAACGGCTTGAATGCTATTGACTCATTATTCACTGAGAAAAAAGCGGCTGCGGTAATTAACGG
GCCATGGGCATTCCAACCGTATGAAGCCGCTGGTATTAACTTTGGTGTGTCACCACTGCCAGCATTGCCGAACGGCAAAG
ATATGAGCTCCTTCCTCGGTGTGAAAGGGTATGTCGTTTCTACCTGGAGCAAAGATAAGGCACTCGCCCAGCAGTTCATC
GAATTTATTAACCAACCGCAATACGTGAAAACCCGCTATCAGGTCACCAAAGAGATCCCCGCGTTGACGGCCATGATTGA
CGATCCATTGATTAAAAATGATGAAAAAGCCAGTGCGGTAGCCATTCAGGCAAGCCGTGCCTCTGCGATGCCTGGTATTC
CAGAAATGGGCGAAGTGTGGGGACCTGCTAACTCAGCATTAGAGCTAAGCGTAACGGGCAAACAGGAGCCTAAAGTCGCT
CTCGATAACGCCGTTAAGCAGATCAATATGCAAATCGAGGCCATGCAGGCCAGTAATCAGTAA

Upstream 100 bases:

>100_bases
ATAACATAGTAAGACATCGGCATGATATGTTCTGCAGATGGCGTAGATGTGGTGTTTGTCATTTTGTATTTGTATTTAAT
TTCGAGCGTTAAACACAATA

Downstream 100 bases:

>100_bases
TCAGACTATTTAACAGGTCAGTATGAATTTAGGTGGTGTTCAATTCCCCCACGTGCCGTTGGTATGCGATGGTTTTTGTG
CACCACCTGTGTTTCAAACT

Product: putative sugar-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MLYLHPRLIMKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSAGIKEAIADFEKQHQVKVNVLEMPYAQQIEK
LRLDGPAGIGPDVLVIPNDQLGGAVVQGLLTPLSVDPTIVTTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLPTPL
ATLDEYAAFSKKQRAENKYGLLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFP
IGTIGDNGLNAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTWSKDKALAQQFI
EFINQPQYVKTRYQVTKEIPALTAMIDDPLIKNDEKASAVAIQASRASAMPGIPEMGEVWGPANSALELSVTGKQEPKVA
LDNAVKQINMQIEAMQASNQ

Sequences:

>Translated_420_residues
MLYLHPRLIMKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSAGIKEAIADFEKQHQVKVNVLEMPYAQQIEK
LRLDGPAGIGPDVLVIPNDQLGGAVVQGLLTPLSVDPTIVTTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLPTPL
ATLDEYAAFSKKQRAENKYGLLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFP
IGTIGDNGLNAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTWSKDKALAQQFI
EFINQPQYVKTRYQVTKEIPALTAMIDDPLIKNDEKASAVAIQASRASAMPGIPEMGEVWGPANSALELSVTGKQEPKVA
LDNAVKQINMQIEAMQASNQ
>Mature_420_residues
MLYLHPRLIMKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSAGIKEAIADFEKQHQVKVNVLEMPYAQQIEK
LRLDGPAGIGPDVLVIPNDQLGGAVVQGLLTPLSVDPTIVTTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLPTPL
ATLDEYAAFSKKQRAENKYGLLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFP
IGTIGDNGLNAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTWSKDKALAQQFI
EFINQPQYVKTRYQVTKEIPALTAMIDDPLIKNDEKASAVAIQASRASAMPGIPEMGEVWGPANSALELSVTGKQEPKVA
LDNAVKQINMQIEAMQASNQ

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=398, Percent_Identity=29.6482412060301, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45376; Mature: 45376

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLHPRLIMKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSAGIKEAIADFE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KQHQVKVNVLEMPYAQQIEKLRLDGPAGIGPDVLVIPNDQLGGAVVQGLLTPLSVDPTIV
HCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEE
TTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLPTPLATLDEYAAFSKKQRAENKYG
EEECCCCEEEEEECCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
LLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFP
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEE
IGTIGDNGLNAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLG
ECCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHC
VKGYVVSTWSKDKALAQQFIEFINQPQYVKTRYQVTKEIPALTAMIDDPLIKNDEKASAV
CCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
AIQASRASAMPGIPEMGEVWGPANSALELSVTGKQEPKVALDNAVKQINMQIEAMQASNQ
EEEEHHCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEEECCCCC
>Mature Secondary Structure
MLYLHPRLIMKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSAGIKEAIADFE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KQHQVKVNVLEMPYAQQIEKLRLDGPAGIGPDVLVIPNDQLGGAVVQGLLTPLSVDPTIV
HCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEE
TTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLPTPLATLDEYAAFSKKQRAENKYG
EEECCCCEEEEEECCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
LLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFP
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEE
IGTIGDNGLNAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLG
ECCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHC
VKGYVVSTWSKDKALAQQFIEFINQPQYVKTRYQVTKEIPALTAMIDDPLIKNDEKASAV
CCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
AIQASRASAMPGIPEMGEVWGPANSALELSVTGKQEPKVALDNAVKQINMQIEAMQASNQ
EEEEHHCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]