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The map label for this gene is murD

Identifier: 218927749

GI number: 218927749

Start: 597578

End: 598894

Strand: Direct

Name: murD

Synonym: YPO0553

Alternate gene names: 218927749

Gene position: 597578-598894 (Clockwise)

Preceding gene: 218927748

Following gene: 218927750

Centisome position: 12.84

GC content: 53.76

Gene sequence:

>1317_bases
ATGGTTGATTATCAGGGTAAAAAAGTGGTCATCATCGGGCTGGGGCTAACCGGCCTTTCCTGCGTTGATTTCTTTATAGC
GCGTGGTGTCACGCCGCGCGTGATGGATACCCGCATCAATCCACCGGGCTTAGATCAATTACCCGAAAGTGTCGAACAGC
ATGTGGGTGATTTGAACCAAGAATGGCTACTGGACGCTGATTTAATTGTTGTTAGCCCCGGTATGGCTCTGGCACATCCT
GCATTAAGTGAAGCCGCCGAGGCCGGTGTCGAGATCATTGGCGATATCGAACTGTTCTGCCGTGAAAATCAGGCACCGGT
TGTGGCTATCACTGGCTCCAACGGCAAAAGTACCGTGACGACGCTGGTCGGTGAGATGGCAAAAGCGGCAGGTTGGTCAG
TGGGGGGCGGCGGCAATATCGGCGTTCCGGCACTGACATTGCTGAAGCAAGATAACCAATTAACTGTCTTGGAATTATCA
AGTTTCCAACTGGAAACCACCCACAGCTTGCGGGCCTCTGCTGCGACGATTCTGAACGTGACGGAAGATCATACTGACCG
TTATCCACTCGGTTTGCAGCAATACCGTGCGGCGAAACTGCGGGTGTATGAAAATGCCAAGGTTTGTGTCGTGAATGCCG
ATGATGCACTCACCATGCCGGTCCGTGGTGCTGATAGCCGTTGCATCAGTTTTGGGGTCGATGTCGGTGACTACCACCTG
AATAAGCAGCAGGGTGAAATTTGGTTACGGGTTCGTGGTGAGAAAGTGCTGAACACCCGTGAAATGAAATTAAGTGGGCG
TCATAACTATACCAATGCGCTGGCCGCCCTGGCATTAGCTGATGCCGTGGGTATCCCACGGGCATCCAGTTTGAAAGCCT
TGACGACCTTTTCCGGCTTGCCACACCGCTTCCAATTGGTGCTTGAGCGCCATGGTGTGCGCTGGATCAACGACTCCAAA
GCGACCAACGTGGGCAGCACTGAAGCCGCGCTGGATGGTTTACAGGTTGATGGCACCTTGCATTTATTGTTGGGGGGCGA
CGGTAAATCTGCTGATTTCTCCGGGTTAACGCACTTCCTGCAAGGGGATCGCATCAAGGTCTACTGTTTTGGCCGCGATG
GCGGTCAATTGGCTGCGTTGCGCCCTGACGTCTCTCAACTTACCGAGACGATGGCGCAGGCTATGGCGTTGGTGGCAAAA
GTTGTGCTACCCGGCGATAGGGTGCTGCTGTCACCTGCGTGTGCCAGCCTGGATCAATTCCGCAGCTTTGAGCATCGTGG
TAATGAGTTTGCTCGTCTGGCAGAGGAGTTAGGCTGA

Upstream 100 bases:

>100_bases
AACTGAAAGGCTGGCCAGAACCACGGGTGATCGTGCGTTTCTGGATTATTTCGCTGATGCTGGTGCTGATTGGCCTGGCG
ACGCTGAAGGTGCGGTAATT

Downstream 100 bases:

>100_bases
TGCGTATGCCGGGGCTGGGGCTGTTTAATACCTTAAAACATTATGTCATGGGGTCGCGGGAGAGCGACACCACCAGCATG
GTGCTTTATGACAGAACCTT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQEWLLDADLIVVSPGMALAHP
ALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVTTLVGEMAKAAGWSVGGGGNIGVPALTLLKQDNQLTVLELS
SFQLETTHSLRASAATILNVTEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGLPHRFQLVLERHGVRWINDSK
ATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTHFLQGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAK
VVLPGDRVLLSPACASLDQFRSFEHRGNEFARLAEELG

Sequences:

>Translated_438_residues
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQEWLLDADLIVVSPGMALAHP
ALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVTTLVGEMAKAAGWSVGGGGNIGVPALTLLKQDNQLTVLELS
SFQLETTHSLRASAATILNVTEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGLPHRFQLVLERHGVRWINDSK
ATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTHFLQGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAK
VVLPGDRVLLSPACASLDQFRSFEHRGNEFARLAEELG
>Mature_438_residues
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQEWLLDADLIVVSPGMALAHP
ALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVTTLVGEMAKAAGWSVGGGGNIGVPALTLLKQDNQLTVLELS
SFQLETTHSLRASAATILNVTEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGLPHRFQLVLERHGVRWINDSK
ATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTHFLQGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAK
VVLPGDRVLLSPACASLDQFRSFEHRGNEFARLAEELG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=438, Percent_Identity=80.8219178082192, Blast_Score=718, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_YERPA (Q1C212)

Other databases:

- EMBL:   CP000308
- RefSeq:   YP_653455.1
- ProteinModelPortal:   Q1C212
- SMR:   Q1C212
- STRING:   Q1C212
- GeneID:   4121566
- GenomeReviews:   CP000308_GR
- KEGG:   ypa:YPA_3548
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK03806
- BioCyc:   YPES360102:YPA_3548-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 46987; Mature: 46987

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQ
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCH
EWLLDADLIVVSPGMALAHPALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVT
HHEECCCEEEECCCHHHHCHHHHHHHHCCCEEEECEEEEEECCCCCEEEEECCCCCHHHH
TLVGEMAKAAGWSVGGGGNIGVPALTLLKQDNQLTVLELSSFQLETTHSLRASAATILNV
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCEEEEHHHHHHHHHEEEEEE
TEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
CCCCCCCCCCCHHHHHHEEEEEECCCEEEEEECCCCEEEECCCCCCCEEEECCCCCCEEC
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGL
CCCCCEEEEEECCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
PHRFQLVLERHGVRWINDSKATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTHFL
CHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCEEECCEEEEEECCCCCCCCHHHHHHHH
QGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAKVVLPGDRVLLSPACASLDQF
CCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
RSFEHRGNEFARLAEELG
HHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MVDYQGKKVVIIGLGLTGLSCVDFFIARGVTPRVMDTRINPPGLDQLPESVEQHVGDLNQ
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCH
EWLLDADLIVVSPGMALAHPALSEAAEAGVEIIGDIELFCRENQAPVVAITGSNGKSTVT
HHEECCCEEEECCCHHHHCHHHHHHHHCCCEEEECEEEEEECCCCCEEEEECCCCCHHHH
TLVGEMAKAAGWSVGGGGNIGVPALTLLKQDNQLTVLELSSFQLETTHSLRASAATILNV
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCEEEEHHHHHHHHHEEEEEE
TEDHTDRYPLGLQQYRAAKLRVYENAKVCVVNADDALTMPVRGADSRCISFGVDVGDYHL
CCCCCCCCCCCHHHHHHEEEEEECCCEEEEEECCCCEEEECCCCCCCEEEECCCCCCEEC
NKQQGEIWLRVRGEKVLNTREMKLSGRHNYTNALAALALADAVGIPRASSLKALTTFSGL
CCCCCEEEEEECCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
PHRFQLVLERHGVRWINDSKATNVGSTEAALDGLQVDGTLHLLLGGDGKSADFSGLTHFL
CHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCEEECCEEEEEECCCCCCCCHHHHHHHH
QGDRIKVYCFGRDGGQLAALRPDVSQLTETMAQAMALVAKVVLPGDRVLLSPACASLDQF
CCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
RSFEHRGNEFARLAEELG
HHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA