Click here to switch to the map view.

The map label for this gene is ilvI [H]

Identifier: 218927736

GI number: 218927736

Start: 584017

End: 585744

Strand: Direct

Name: ilvI [H]

Synonym: YPO0539

Alternate gene names: 218927736

Gene position: 584017-585744 (Clockwise)

Preceding gene: 218927735

Following gene: 218927737

Centisome position: 12.55

GC content: 49.77

Gene sequence:

>1728_bases
ATGGAGATGTTGTCAGGAGCCGAGATGGTGGTCCGATCGTTAATTGATCAGGGCGTTAAGCATGTATTCGGTTATCCCGG
CGGGGCCGTACTTGATATTTACGATGCCCTGCACACGGTCGGAGGCATCGATCACGTGCTGGTGCGCCATGAACAAGGTG
CGGTTCACATGGCCGATGGTTATGCGCGGGCAACGGGCGAGGTCGGTGTTGTACTGGTCACGTCTGGTCCTGGTGCAACC
AATGCGATTACCGGTATTGCGACCGCCTATATGGATTCAGTGCCAATGGTGGTGCTTTCAGGCCAAGTGCCAAGCTCATT
GATTGGTTTTGATGCCTTCCAAGAATGTGACATGGTGGGGATCTCCCGCCCAGTGGTCAAACATAGCTTCTTGGTTAAGC
GCACAGAAGACATCCCGATGGTGTTGAAAAAAGCCTTTTATCTGGCATCCACTGGCCGTCCCGGTCCGGTGGTTATCGAC
TTGCCAAAAGACATTGTCGGCCCAGCAGTAAAAATGCCTTACGCCTATCCAGAGCAAGTCAGTTTGCGCTCCTATAATCC
TACGGTGCAAGGCCACCGTGGGCAGATTAAGCGTGCACTGCAAACGATTATGGCGGCTAAACGGCCCATTATGTATGTCG
GTGGTGGTGCGATTAACGCCGCTTGCCATGAAGAATTACTGATATTGGCAGAGAAGCTGAACTTACCGGTAACCAGCTCT
CTGATGGGGCTAGGCAGTTTCCCTGGTACGCATCGCCAAAGTGTCGGCATGTTGGGGATGCATGGTACCTTTGAAGCCAA
TATGGCCATGCACAATACTGATCTGATTTTTGCCGTCGGTGTCCGTTTTGACGATCGTACCACCAATAATTTAGCAAAAT
ATTGCCCTGATGCCACAGTGCTACACATTGATATCGATCCGACATCGATCTCGAAAACGGTCACCGCCGATATCCCGATT
GTGGGTGATGCCAAACAAGTACTGACACAAATGTTAGATTTGTTGTCGCAGGTCGGCTGTACACAAGATTTTGATAGCTT
GCGCGATTGGTGGCAATCCATTGAACAATGGCGCACCCGTAATTGCTTGGGCTACAACAAAGACAGTGGGAAAATCAAGC
CACAAGCGGTGATTGAAGCCTTACATCGTCTGACTAAAGGCGATGCGTACGTTACCTCTGATGTGGGCCAACATCAGATG
TTTGCGGCTCTTTACTATCCCTTTGATAAGCCACGTCGTTGGATTAACTCCGGTGGCTTGGGCACGATGGGCTTTGGCTT
GCCAGCGGCGCTTGGGGTTAAATTGGCTTTGCCTAATGAAACCGTCGTTTGTGTGACCGGTGATGGCAGTATCCAGATGA
ATATTCAGGAGTTATCAACCGCACTGCAATATAACTTGCCAGTGTTGGTATTAAACCTGAATAACCGCTATCTGGGGATG
GTGAAACAATGGCAGGACATGATTTATTCAGGCCGCCATTCTCAGTCGTATATGGATTCGCTGCCTGATTTTGTCAAGTT
GGCTGAAGCCTATGGCCATATTGGTGTGGCTATTAATACGCCGGATGAGCTGGAAAGTAAGCTGGCAGACGCACTGACAC
AATTATCCGAAACCAATCGTTTGGTCTTTGTGGATGTGACTGTCGATGAAACAGAACATGTTTACCCGATGCAGATTCGT
GGTGGTGGCATGGACGAAATGTGGCTAAGCAAAACGGAGAGGACTTAA

Upstream 100 bases:

>100_bases
CCACAAATAAGAATAAAGAGGCGTTGCGCCTTTCCTACAGAATATCGTGGAAATGAATTCCCGTTATCCAATGAGCAAAC
TAAGCCCGGAGGCAAAACCC

Downstream 100 bases:

>100_bases
TTATGCGCCGCCGGATTTTATCAGTACTGCTGGAAAATGAGTCAGGTGCACTCTCACGGGTGGTGGGGCTGTTTTCTCAG
CGTGGCTATAATATCGAAAG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQGAVHMADGYARATGEVGVVLVTSGPGAT
NAITGIATAYMDSVPMVVLSGQVPSSLIGFDAFQECDMVGISRPVVKHSFLVKRTEDIPMVLKKAFYLASTGRPGPVVID
LPKDIVGPAVKMPYAYPEQVSLRSYNPTVQGHRGQIKRALQTIMAAKRPIMYVGGGAINAACHEELLILAEKLNLPVTSS
LMGLGSFPGTHRQSVGMLGMHGTFEANMAMHNTDLIFAVGVRFDDRTTNNLAKYCPDATVLHIDIDPTSISKTVTADIPI
VGDAKQVLTQMLDLLSQVGCTQDFDSLRDWWQSIEQWRTRNCLGYNKDSGKIKPQAVIEALHRLTKGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKLALPNETVVCVTGDGSIQMNIQELSTALQYNLPVLVLNLNNRYLGM
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHIGVAINTPDELESKLADALTQLSETNRLVFVDVTVDETEHVYPMQIR
GGGMDEMWLSKTERT

Sequences:

>Translated_575_residues
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQGAVHMADGYARATGEVGVVLVTSGPGAT
NAITGIATAYMDSVPMVVLSGQVPSSLIGFDAFQECDMVGISRPVVKHSFLVKRTEDIPMVLKKAFYLASTGRPGPVVID
LPKDIVGPAVKMPYAYPEQVSLRSYNPTVQGHRGQIKRALQTIMAAKRPIMYVGGGAINAACHEELLILAEKLNLPVTSS
LMGLGSFPGTHRQSVGMLGMHGTFEANMAMHNTDLIFAVGVRFDDRTTNNLAKYCPDATVLHIDIDPTSISKTVTADIPI
VGDAKQVLTQMLDLLSQVGCTQDFDSLRDWWQSIEQWRTRNCLGYNKDSGKIKPQAVIEALHRLTKGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKLALPNETVVCVTGDGSIQMNIQELSTALQYNLPVLVLNLNNRYLGM
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHIGVAINTPDELESKLADALTQLSETNRLVFVDVTVDETEHVYPMQIR
GGGMDEMWLSKTERT
>Mature_575_residues
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQGAVHMADGYARATGEVGVVLVTSGPGAT
NAITGIATAYMDSVPMVVLSGQVPSSLIGFDAFQECDMVGISRPVVKHSFLVKRTEDIPMVLKKAFYLASTGRPGPVVID
LPKDIVGPAVKMPYAYPEQVSLRSYNPTVQGHRGQIKRALQTIMAAKRPIMYVGGGAINAACHEELLILAEKLNLPVTSS
LMGLGSFPGTHRQSVGMLGMHGTFEANMAMHNTDLIFAVGVRFDDRTTNNLAKYCPDATVLHIDIDPTSISKTVTADIPI
VGDAKQVLTQMLDLLSQVGCTQDFDSLRDWWQSIEQWRTRNCLGYNKDSGKIKPQAVIEALHRLTKGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKLALPNETVVCVTGDGSIQMNIQELSTALQYNLPVLVLNLNNRYLGM
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHIGVAINTPDELESKLADALTQLSETNRLVFVDVTVDETEHVYPMQIR
GGGMDEMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=595, Percent_Identity=27.0588235294118, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI21361361, Length=585, Percent_Identity=24.7863247863248, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=84, Blast_Score=1017, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=40, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=33.2155477031802, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1787096, Length=561, Percent_Identity=29.2335115864528, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788716, Length=580, Percent_Identity=25.1724137931034, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=547, Percent_Identity=26.691042047532, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17531299, Length=547, Percent_Identity=26.691042047532, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=599, Percent_Identity=24.7078464106845, Blast_Score=145, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=586, Percent_Identity=43.0034129692833, Blast_Score=457, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=548, Percent_Identity=24.8175182481752, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321524, Length=577, Percent_Identity=23.050259965338, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=473, Percent_Identity=23.0443974630021, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=489, Percent_Identity=23.9263803680982, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922626, Length=596, Percent_Identity=26.1744966442953, Blast_Score=185, Evalue=6e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62732; Mature: 62732

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQGAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEECC
YARATGEVGVVLVTSGPGATNAITGIATAYMDSVPMVVLSGQVPSSLIGFDAFQECDMVG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHCCCCC
ISRPVVKHSFLVKRTEDIPMVLKKAFYLASTGRPGPVVIDLPKDIVGPAVKMPYAYPEQV
CCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCCC
SLRSYNPTVQGHRGQIKRALQTIMAAKRPIMYVGGGAINAACHEELLILAEKLNLPVTSS
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHCCCCHHHH
LMGLGSFPGTHRQSVGMLGMHGTFEANMAMHNTDLIFAVGVRFDDRTTNNLAKYCPDATV
HHHCCCCCCCCCCCCCEEECCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVTADIPIVGDAKQVLTQMLDLLSQVGCTQDFDSLRDWWQSIEQWRTR
EEEECCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
NCLGYNKDSGKIKPQAVIEALHRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHEEHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKLALPNETVVCVTGDGSIQMNIQELSTALQYNLPVLVLNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHIGVAINTPDELESKLADALTQLSETNR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCC
LVFVDVTVDETEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MEMLSGAEMVVRSLIDQGVKHVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQGAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEECC
YARATGEVGVVLVTSGPGATNAITGIATAYMDSVPMVVLSGQVPSSLIGFDAFQECDMVG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHCCCCC
ISRPVVKHSFLVKRTEDIPMVLKKAFYLASTGRPGPVVIDLPKDIVGPAVKMPYAYPEQV
CCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCCC
SLRSYNPTVQGHRGQIKRALQTIMAAKRPIMYVGGGAINAACHEELLILAEKLNLPVTSS
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHCCCCHHHH
LMGLGSFPGTHRQSVGMLGMHGTFEANMAMHNTDLIFAVGVRFDDRTTNNLAKYCPDATV
HHHCCCCCCCCCCCCCEEECCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVTADIPIVGDAKQVLTQMLDLLSQVGCTQDFDSLRDWWQSIEQWRTR
EEEECCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
NCLGYNKDSGKIKPQAVIEALHRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHEEHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKLALPNETVVCVTGDGSIQMNIQELSTALQYNLPVLVLNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMDSLPDFVKLAEAYGHIGVAINTPDELESKLADALTQLSETNR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCC
LVFVDVTVDETEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]