Click here to switch to the map view.

The map label for this gene is purA

Identifier: 218906659

GI number: 218906659

Start: 5282575

End: 5283864

Strand: Reverse

Name: purA

Synonym: BCAH820_5577

Alternate gene names: 218906659

Gene position: 5283864-5282575 (Counterclockwise)

Preceding gene: 218906660

Following gene: 218906658

Centisome position: 99.65

GC content: 39.15

Gene sequence:

>1290_bases
ATGTCTTCAGTAGTAGTTGTAGGAACACAATGGGGCGACGAAGGAAAAGGTAAAATTACTGATTTTCTTTCTGAGCATGC
GGAAGTAGTTGCAAGATATCAAGGTGGAAATAACGCAGGACATACAATCGTTTTTGGTGGAGTTAAATATAAATTACACT
TAATTCCATCTGGTATTTTCTATAAAGAGAAAATTTGTGTAATCGGAAACGGTTTAGTAGTAGATCCGAAAGCATTACTT
GAAGAGTTAAAATACTTACACGATCGTGGTGTAAGTACTGATAATTTACGCGTAAGTAACCGTGCGCACGTTATTTTACC
TTATCACTTAAAACAGGACGAGTTAGAAGAAGCAAGTAAAGGTGATAACAAAATCGGTACAACGAAAAAAGGTATCGGTC
CTGCATATATGGACAAAGCTGCTCGTATCGGTATTCGTATGGCTGATCTTTTAGACCGTGAAGCATTTAAAGAGAAGCTT
GAGCAAAATTTAGCACAAAAAAATCGTTTATTTGAAAAAATGTACGATACAGAAGGTTTCAGTGTAGACGAAATCTTCGA
AGAGTACTTCGAGTACGGACAGCAAATCGCACAATATGTATGTGATACGTCTGTTGTATTAAATGATGCATTAGATAACA
ATCACCGTGTATTATTTGAAGGTGCACAAGGTGTTATGCTTGATATCGACCACGGTACGTACCCGTTCGTTACATCTTCT
AACCCAATTGCTGGTGGTGTAACAGTTGGAACTGGAGTTGGTCCTGCGAAAGTTACTCGTGTTGTAGGTGTATGTAAAGC
ATATACAAGCCGCGTTGGTGACGGTCCATTCCCTACTGAGCTTCATGACGAAATTGGTCATCAAATTCGTGAAGTTGGTC
GTGAGTATGGAACGACAACTGGTCGTCCACGCCGCGTAGGTTGGTTCGATAGCGTTGTTGTAAGACACGCACGTCGTGTT
AGTGGTTTAACAGATCTATCATTAAACTCTATCGACGTTCTAACTGGTATTCCAACACTTAAAATTTGTGTTGCTTACAA
ATGCGATGGCAAAGTTATCGATGAAGTTCCAGCAAACTTAAACATTTTAGCGAAATGTGAGCCTGTATACGAAGAGCTTC
CAGGTTGGACAGAAGATATTACTGGTGTAAGATCATTAGATGAGCTTCCTGAAAATGCACGAAAATACGTAGAACGTGTT
TCTGAGTTAACAGGAATTCAATTATCTATGTTCTCAGTTGGACCAGATCGTAACCAAACAAATATCGTTCGTAACGTATA
CGAAGCTTAA

Upstream 100 bases:

>100_bases
TTATAAAAAAATGTTCGCTTTTTGGTTGACTTCTTTTTCCGTTTTGGTACAATTATATTGTTTGAATAGATCGTTTGAAA
ATTGCGGAGGTGCTTTAATA

Downstream 100 bases:

>100_bases
TTGATATTTTTGAGAAAAACTCATGTCCGCATGAGTTTTTTCTTATCTTTTATAAAAAAATAAAAAAAGGTATTGCGAAA
TATAAAGAAAACATGTTATG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIFYKEKICVIGNGLVVDPKALL
EELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASKGDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKL
EQNLAQKNRLFEKMYDTEGFSVDEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERV
SELTGIQLSMFSVGPDRNQTNIVRNVYEA

Sequences:

>Translated_429_residues
MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIFYKEKICVIGNGLVVDPKALL
EELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASKGDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKL
EQNLAQKNRLFEKMYDTEGFSVDEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERV
SELTGIQLSMFSVGPDRNQTNIVRNVYEA
>Mature_428_residues
SSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIFYKEKICVIGNGLVVDPKALLE
ELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASKGDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKLE
QNLAQKNRLFEKMYDTEGFSVDEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSSN
PIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTTGRPRRVGWFDSVVVRHARRVS
GLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERVS
ELTGIQLSMFSVGPDRNQTNIVRNVYEA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=422, Percent_Identity=42.8909952606635, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI22748717, Length=421, Percent_Identity=43.2304038004751, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=42.0918367346939, Blast_Score=292, Evalue=5e-79,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=46.9767441860465, Blast_Score=410, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=42.1911421911422, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=42.1911421911422, Blast_Score=330, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=41.371158392435, Blast_Score=341, Evalue=2e-94,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=42.9256594724221, Blast_Score=335, Evalue=5e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_BACAH (A0RLP6)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_897646.1
- ProteinModelPortal:   A0RLP6
- SMR:   A0RLP6
- STRING:   A0RLP6
- EnsemblBacteria:   EBBACT00000066570
- GeneID:   4543713
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_4974
- eggNOG:   COG0104
- GeneTree:   EBGT00050000001253
- HOGENOM:   HBG658237
- ProtClustDB:   PRK01117
- BioCyc:   BTHU412694:BALH_4974-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47424; Mature: 47293

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2; PS00178 AA_TRNA_LIGASE_I

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIF
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCE
YKEKICVIGNGLVVDPKALLEELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASK
EECEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCCHHHHHHHHC
GDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKLEQNLAQKNRLFEKMYDTEGF
CCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVDEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCCHHEEHHCCCCCCEEEEECCCEEEEEECCCCCCEEECC
NPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCHHCCCCCC
NILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERVSELTGIQLSMFSVGPDRNQT
EEEEECCHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
NIVRNVYEA
HHHHHHHCC
>Mature Secondary Structure 
SSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKYKLHLIPSGIF
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCE
YKEKICVIGNGLVVDPKALLEELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASK
EECEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCCHHHHHHHHC
GDNKIGTTKKGIGPAYMDKAARIGIRMADLLDREAFKEKLEQNLAQKNRLFEKMYDTEGF
CCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVDEIFEEYFEYGQQIAQYVCDTSVVLNDALDNNHRVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHCCHHEEHHCCCCCCEEEEECCCEEEEEECCCCCCEEECC
NPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCHHCCCCCC
NILAKCEPVYEELPGWTEDITGVRSLDELPENARKYVERVSELTGIQLSMFSVGPDRNQT
EEEEECCHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHH
NIVRNVYEA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA