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The map label for this gene is amiF

Identifier: 218905070

GI number: 218905070

Start: 3790790

End: 3791788

Strand: Reverse

Name: amiF

Synonym: BCAH820_3954

Alternate gene names: 218905070

Gene position: 3791788-3790790 (Counterclockwise)

Preceding gene: 218905071

Following gene: 218905068

Centisome position: 71.51

GC content: 37.14

Gene sequence:

>999_bases
ATGGGTAGTAGTGGAAGTATGGTAAAGCCAATTAGTGGGTTTTTAACAGCATTAATACAATATCCAGTACCGGTAGTAGA
GTCACGTGCAGACATTGATAAACAAATTAAACAGATCATAAAAACAATTCATTCAACTAAAGCAGGGTATCCTGGATTGG
AATTGATAGTATTTCCAGAATATAGTACGCAAGGGCTTAATACAAAAAAATGGACTACAGAAGAGTTTTTATGTACAGTC
CCTGGGCCAGAAACAGACTTGTTTGCTGAGGCATGTAAAGAGTCTGAAGTATATGGTGTTTTTTCTATAATGGAAAGAAA
TCCTGATGGTGGAGAACCGTATAATACAGCAATAATTATTGATCCACAAGGTGAAATGATTTTGAAGTATCGTAAGCTAA
ATCCTTGGGTGCCAGTTGAACCTTGGAAGGCTGGAGATTTAGGTTTACCTGTTTGTGATGGACCGGGAGGAAGTAAATTA
GCTGTTTGTATTTGTCATGATGGTATGTTTCCTGAAGTAGCTCGTGAAGCAGCTTATAAAGGTGCAAATGTCTTGATTCG
TATTTCTGGATATAGCACACAAGTTAGTGAACAATGGATGTTAACTAATCGTTCGAATGCATGGCAAAATTTAATGTATA
CATTGTCGGTGAACTTAGCGGGTTATGATGGTGTATTTTATTATTTTGGTGAAGGACAAGTATGTAATTTTGATGGGACT
ACTTTAGTGCAGGGACACCGAAATCCTTGGGAGATTGTTACTGCTGAAGTATATCCAGAGCTAGCAGATCAAGCTAGATT
AGGATGGGGTCTGGAAAATAATATATATAATTTAGGTTCAAGGGGATATGTAGCAACTCCTGGTGGGGTGAAAGAAAACC
CGTATACGTTTGTAAAAGATTTAGCTGAAGGTAAATATAAGGTTCCTTGGGAAGATGAGATTAAAGTAAAGGATGGAACT
ATTTATGGGTATCCAGTCAAAAAGACGATTCATTCTTAA

Upstream 100 bases:

>100_bases
AATACATAATAATTCCATGTGTAGTACAAGTTTTTTAGAGAGTTACAAATTTATATTGTTATCTTTAATAAAACAAGGAC
TATTTTAAGGGGGAATTTAA

Downstream 100 bases:

>100_bases
ATAAGATAAATAGAACAAGCGCATCATATTGATGCGCTTGTTCTATTTACTCAATGATTTGGAATTCTTTTAACTCTTTT
TCGATATTTTGTAGGAGTTG

Product: formamidase

Products: NA

Alternate protein names: Formamide amidohydrolase

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MGSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIKQIIKTIHSTKAGYPGLELIVFPEYSTQGLNTKKWTTEEFLCTV
PGPETDLFAEACKESEVYGVFSIMERNPDGGEPYNTAIIIDPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKL
AVCICHDGMFPEVAREAAYKGANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGT
TLVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKDLAEGKYKVPWEDEIKVKDGT
IYGYPVKKTIHS

Sequences:

>Translated_332_residues
MGSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIKQIIKTIHSTKAGYPGLELIVFPEYSTQGLNTKKWTTEEFLCTV
PGPETDLFAEACKESEVYGVFSIMERNPDGGEPYNTAIIIDPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKL
AVCICHDGMFPEVAREAAYKGANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGT
TLVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKDLAEGKYKVPWEDEIKVKDGT
IYGYPVKKTIHS
>Mature_331_residues
GSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIKQIIKTIHSTKAGYPGLELIVFPEYSTQGLNTKKWTTEEFLCTVP
GPETDLFAEACKESEVYGVFSIMERNPDGGEPYNTAIIIDPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKLA
VCICHDGMFPEVAREAAYKGANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGTT
LVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKDLAEGKYKVPWEDEIKVKDGTI
YGYPVKKTIHS

Specific function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMIF_BACAA (C3P6U6)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868239.1
- ProteinModelPortal:   C3P6U6
- SMR:   C3P6U6
- EnsemblBacteria:   EBBACT00000129981
- GeneID:   7852389
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4174
- GeneTree:   EBGT00050000001846
- ProtClustDB:   PRK13287
- HAMAP:   MF_01243
- InterPro:   IPR022843
- InterPro:   IPR003010
- Gene3D:   G3DSA:3.60.110.10

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: =3.5.1.49

Molecular weight: Translated: 36808; Mature: 36677

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50263 CN_HYDROLASE

Important sites: ACT_SITE 60-60 ACT_SITE 132-132 ACT_SITE 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIKQIIKTIHSTKAGYPGLELIVFPE
CCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
YSTQGLNTKKWTTEEFLCTVPGPETDLFAEACKESEVYGVFSIMERNPDGGEPYNTAIII
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCEEEEE
DPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKLAVCICHDGMFPEVAREAAYK
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHCC
GANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGT
CCEEEEEEECCCCCCCCEEEEECCCCHHHEEEEEEEEEEECCCCEEEEECCCEEECCCCC
TLVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKD
EEEECCCCCCEEEEHHHCHHHHHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHH
LAEGKYKVPWEDEIKVKDGTIYGYPVKKTIHS
HHCCCEECCCCCCEEEECCEEEECCHHHHCCC
>Mature Secondary Structure 
GSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIKQIIKTIHSTKAGYPGLELIVFPE
CCCCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
YSTQGLNTKKWTTEEFLCTVPGPETDLFAEACKESEVYGVFSIMERNPDGGEPYNTAIII
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCEEEEE
DPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKLAVCICHDGMFPEVAREAAYK
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHCC
GANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGT
CCEEEEEEECCCCCCCCEEEEECCCCHHHEEEEEEEEEEECCCCEEEEECCCEEECCCCC
TLVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKD
EEEECCCCCCEEEEHHHCHHHHHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHH
LAEGKYKVPWEDEIKVKDGTIYGYPVKKTIHS
HHCCCEECCCCCCEEEECCEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA